Experimental Modeling of Flavonoid–Biomembrane Interactions

Sanver, Didem; Murray, Brent S.; Sadeghpour, Amin; Rappolt, Michael; Nelson, Andrew

doi:10.1021/acs.langmuir.6b02219

Cited by 72 publications

(52 citation statements)

References 66 publications

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“…Our hypothesis is also supported by lower proton leak across the IMM and an overall decrease in DCF fluorescence, albeit the latter is not exclusively indicative of ROS alone nor informative of specific ROS species formed [73], but limitations in this assay are outweighed by the highly significant differences observed in cells treated with quercetin and is the decrease is associated with the general change in redox status and lower oxidative stress. Previous studies have shown that quercetin improves mitochondrial bioenergetics in various other models [22,23], while a role in maintaining membrane integrity has also been hypothesized [74][75][76]. The lower Leak in high glucosetreated cells with quercetin led to enhanced mitochondrial respiration by maximizing coupling efficiency and was reflected in higher Routine and net ETS respiration, indicative of increased use of ETS capacity for ATP turnover [54] as found here.…”

Section: Discussionsupporting

confidence: 72%

Quercetin preserves redox status and stimulates mitochondrial function in metabolically-stressed HepG2 cells

Houghton

Kerimi

Tumova

et al. 2018

Free Radical Biology and Medicine

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ReuseThis article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) licence. This licence only allows you to download this work and share it with others as long as you credit the authors, but you can't change the article in any way or use it commercially. More information and the full terms of the licence here: https://creativecommons.org/licenses/ TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. AbstractHyperglycemia augments formation of intracellular reactive oxygen species (ROS) with associated mitochondrial damage and increased risk of insulin resistance in type 2 diabetes. We examined whether quercetin could reverse chronic high glucose-induced oxidative stress and mitochondrial dysfunction. Following long-term high glucose treatment, complex I activity was significantly decreased in isolated mitochondria from HepG2 cells. Quercetin dose-dependently recovered complex I activity and lowered cellular ROS generation under both high and normal glucose conditions. Respirometry studies showed that quercetin could counteract the detrimental increase in inner mitochondrial membrane proton leakage resulting from high glucose while it increased oxidative respiration, despite a decrease in electron transfer system (ETS) capacity, and lower non-ETS oxygen consumption. A quercetin-stimulated increase in cellular NAD + /NADH was evident within 2 h and a two-fold increase in PGC-1 mRNA within 6 h, in both normal and high glucose conditions. A similar pattern was also found for the mRNA expression of the repulsive guidance molecule b (RGMB) and its long non-coding RNA (lncRNA) RGMB-AS1 with quercetin, indicating a potential change of the glycolytic phenotype and suppression of aberrant cellular growth which is characteristic of the HepG2 cells. Direct effects of quercetin on PGC-1 activity were minimal, as quercetin only weakly enhanced PGC-1 binding to PPAR in vitro at higher concentrations. Our results suggest that quercetin may protect mitochondrial function from high glucose-induced stress by increasing cellular NAD + /NADH and activation of PGC-1 -mediated pathways. Lower ROS in combination with improved complex I activity and ETS coupling efficiency under conditions of amplified oxidative stress could reinforce mitochondrial integrity and improve redox status, beneficial in certain metabolic diseases. Abbreviations oxygen species; ROXresidual oxygen consumption; SIRTsirtuin; TBP -TATA-box binding protein; TFAMmitochondrial transcription factor A; TR-FRETtime-resolved fluorescence energy transfer.Graphical Abstract:

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Section: Discussionsupporting

confidence: 72%

Quercetin preserves redox status and stimulates mitochondrial function in metabolically-stressed HepG2 cells

Houghton

Kerimi

Tumova

et al. 2018

Free Radical Biology and Medicine

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“…Flavonoids are hydroxylated phenolic substances which are also able to complex with bacterial cell walls and disrupt microbial membranes [75,105]. Highly active flavonoids, quercetin (1), rutin (2), naringenin (3), sophoraflavanone (4), tiliroside (5) and 2, 4, 6trihydroxy-30-methyl chalcone (6) ( Figure 2) decreased lipid bilayer thickness and fluidity levels and increased membrane permeability, supporting the leaking of intracellular protein and ions in S. aureus and S. mutans [112,113]. These compounds contribute to the synergistic effect with ampicillin and tetracycline [114].…”

Section: Promote Cell Wall Disruption and Lysismentioning

confidence: 84%

Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens

Mickymaray

2019

Antibiotics

118

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Traditional medicinal plants have been cultivated to treat various human illnesses and avert numerous infectious diseases. They display an extensive range of beneﬁcial pharmacological and health effects for humans. These plants generally synthesize a diverse range of bioactive compounds which have been established to be potent antimicrobial agents against a wide range of pathogenic organisms. Various research studies have demonstrated the antimicrobial activity of traditional plants scientifically or experimentally measured with reports on pathogenic microorganisms resistant to antimicrobials. The antimicrobial activity of medicinal plants or their bioactive compounds arising from several functional activities may be capable of inhibiting virulence factors as well as targeting microbial cells. Some bioactive compounds derived from traditional plants manifest the ability to reverse antibiotic resistance and improve synergetic action with current antibiotic agents. Therefore, the advancement of bioactive-based pharmacological agents can be an auspicious method for treating antibiotic-resistant infections. This review considers the functional and molecular roles of medicinal plants and their bioactive compounds, focusing typically on their antimicrobial activities against clinically important pathogens.

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“…The inhibitory effects of gallotannins may be attributed to their iron-complexing properties and ability to interact with proteins and inhibit enzyme activities [79]. At the same time, flavonoids have a series of antibacterial actions with different mechanisms of action, such as inhibition of nucleic acid synthesis [80], induction of cytoplasmic membrane damage [81,82] and inhibition of energy metabolism [83], biofilm formation [3] and bacterial toxin production [84]. The flavonoid catechin can penetrate the lipid bilayers of the membrane resulting in leakage of intramembranous materials and liposome aggregation [85,86].…”

Section: Foodborne Pathogens and Food Spoilage Organismsmentioning

confidence: 99%

Plant Phenolics and Phenolic-Enriched Extracts as Antimicrobial Agents against Food-Contaminating Microorganisms

et al. 2020

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Phenolic compounds and extracts with bioactive properties can be obtained from many kinds of plant materials. These natural substances have gained attention in the food research as possible growth inhibitors of foodborne pathogenic and spoilage bacteria. Many phenolic-enriched plant extracts and individual phenolics have promising anti-quorum sensing potential as well and can suppress the biofilm formation and toxin production of food-related pathogens. Various studies have shown that plant phenolics can substitute or support the activity of synthetic food preservatives and disinfectants, which, by the way, can provoke serious concerns in consumers. In this review, we will provide a brief insight into the bioactive properties, i.e., the antimicrobial, anti-quorum sensing, anti-biofilm and anti-enterotoxin activities, of plant phenolic extracts and compounds, with special attention to pathogen microorganisms that have food relation. Carbohydrase aided applications to improve the antimicrobial properties of phenolic extracts are also discussed.

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Experimental Modeling of Flavonoid–Biomembrane Interactions

Cited by 72 publications

References 66 publications

Quercetin preserves redox status and stimulates mitochondrial function in metabolically-stressed HepG2 cells

Quercetin preserves redox status and stimulates mitochondrial function in metabolically-stressed HepG2 cells

Efficacy and Mechanism of Traditional Medicinal Plants and Bioactive Compounds against Clinically Important Pathogens

Plant Phenolics and Phenolic-Enriched Extracts as Antimicrobial Agents against Food-Contaminating Microorganisms

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