Experimental models of scald burns. A scope review

Menegat, Taís Amadio; Oliveira, Aline Feitoza de; Majewski, Michelle Gioia Coiado; Blanes, Leila; Juliano, Yára; Novo, Neil Ferreira; Ferreira, Lydia Masako

doi:10.1590/s0102-865020190100000007

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…Although this method seemed to provide relatively reproducible burns, immersing animals in hot water remains an ethical concern. In addition, a review by Menegat et al, reported that there were some discrepancies in burn creation that were reported in scald burn models (Menegat et al, 2019). Furthermore, creating multiple wounds on an animal with this methodology is challenging.…”

Section: Scald Burnmentioning

confidence: 99%

Sustained drug delivery to reduce the extent of burn progression

Moogaambikai

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I would like to thank my co-supervisors Prof. David Leavesley and Asst Prof. Franklin Zhong for their unwavering support during this process. A special thank you to my mentor, Prof. Anthony Phillips, for all of his insightful advice and helpful criticism during the course of my candidature.A huge thank you to Assoc Prof. Andrew Tan and Asst Prof. Navin Verma from the thesis advisory committee for their continuous support and guidance throughout my thesis. I would like to thank my "pre-doc" supervisor from Singapore General Hospital, Dr. Feng Jiajun, for his invaluable support and the Singapore General Hospital, Burns Unit for letting me shadow them as part of my clinical attachment to observe burn victims.

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Section: Scald Burnmentioning

confidence: 99%

Sustained drug delivery to reduce the extent of burn progression

Moogaambikai

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Experimental Models for Controlled Burn Injuries in Rats: A Systematic Analysis of Original Methods and Burn Devices

Morărașu

Morarasu

Gheţu

et al. 2021

Journal of Burn Care &Amp; Research

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AIM Despite a wide variety of models found in literature, choosing the right one can be difficult as many of them are lacking precise methodology. This study aims to analyze and compare original burn models in terms of burn device and technique, parameters, and wound depth assessment. METHODS A systematic search was performed according to PRISMA guidelines on studies describing original experimental burn models in rats. The adapted PICO formula and ARRIVE checklist were followed for inclusion and assessment of quality of studies. Characteristics of animals, burn technique, burn parameters and method of histological confirmation of burn depth were recorded. RESULTS Twenty-seven studies were included in the final analysis. Most studies used direct contact with skin for burn infliction (n=20). The rat’s dorsum was the most common site (n=18). Ten studies used manually controlled burn devices, while ten designed automatic burn devices with control over temperature (n=10), exposure time (n=5), and pressure (n=5). Most studies (n=7) used a single biopsy taken from the center of the wound to confirm burn depth immediately after burn infliction. CONCLUSION From the wide variety of burn models in current literature, our study provides an overview of the most relevant experimental burn models in rats aiding researchers to understand what needs to be addressed when designing their burn protocol. Models cannot be compared as burn parameters variate significantly. Assessment of burn depth should be done in a standardized, sequential fashion in future burn studies to increase reproducibility.

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Translational potential of test systems in modelling thermal burn wounds

Sultanova,

Kryshen',

Makarova

2024

Transl. med. (Print)

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The article describes the advantages and features of experimental models of thermal burns using in vitro, ex vivo and in vivo test systems. An objective assessment of the application of each approach depending on the type of study is given. For example, cell culture models are simple but do not fully reflect the structure of human skin, which limits their translational value. Ex vivo models, such as skin explants, provide the necessary architectonics to study intercellular interactions, but they also have drawbacks, primarily related to short viability. In general, in vitro and ex vivo models have limitations in reproducing all aspects of burn wound pathogenesis and healing. In this regard, laboratory animals, primarily mice, rats, and pigs, are widely used to study burn wound pathology, its effects on the body, and the efficacy of therapy. The decision to use experimental animal models is made taking into account their translational relevance to humans. In rodents, wound healing occurs mainly by contraction, in contrast to the re-epithelialisation and granulation seen in humans, which contributes to faster wound healing in rodents. The significant similarities between certain properties of pig and human skin make the latter a relevant test system in pharmacodynamic studies of thermal burn wounds.

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Experimental models of scald burns. A scope review

Cited by 3 publications

References 25 publications

Sustained drug delivery to reduce the extent of burn progression

Sustained drug delivery to reduce the extent of burn progression

Experimental Models for Controlled Burn Injuries in Rats: A Systematic Analysis of Original Methods and Burn Devices

Translational potential of test systems in modelling thermal burn wounds

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