2003
DOI: 10.1007/s00423-003-0426-y
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Experimental models to study microcirculatory dysfunction in muscle ischemia?reperfusion and osteomyocutaneous flap transfer

Abstract: These experimental results not only demonstrate the importance of the use of advanced in vivo methods to delineate pathophysiological mechanisms in complex disease models, but may also provide a basis for potential prospective randomized trials to test the benefit for the patient in the daily clinical routine.

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Cited by 23 publications
(17 citation statements)
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“…It is likely that the application of heat caused endothelial cell damage at the vascular endothelium and induced an increased inflammatory tissue response [25]. This supports the findings reported by Menger et al, who detected an endothelin (ET)-1-mediated local inflammatory response after microvascular constriction following heat application [26]. This led to perfusion failures in microvessels [27].…”
Section: Discussionsupporting
confidence: 67%
“…It is likely that the application of heat caused endothelial cell damage at the vascular endothelium and induced an increased inflammatory tissue response [25]. This supports the findings reported by Menger et al, who detected an endothelin (ET)-1-mediated local inflammatory response after microvascular constriction following heat application [26]. This led to perfusion failures in microvessels [27].…”
Section: Discussionsupporting
confidence: 67%
“…We did consider perfusing each target tissue following sacrifice with the idea of flushing out leukocytes and identifying labeled cells. We decided against this because in our experience, and in the experience of other researchers (Durowicz and Olszewski, 2003; Menger et al, 2003), such procedures under the unphysiological condition of anesthesia introduce substantial additional sampling error, given the profound alterations in vascular resistance, adhesion molecule production by endothelium, and distribution that occur within individual tissue. Since the animals were passively exsanguinated, any labeled cells that we found within an organ would predominantly be those that were not freely circulating but likely marginated.…”
Section: Discussionmentioning
confidence: 99%
“…In brief, after intraperitoneal anesthesia and removal of the fur, a laterally based myocutaneous flap with a width-to-length ratio of 15 ϫ 11 mm, including the panniculus carnosus, was elevated perpendicularly to the spine, transsecting both the deep circumflex iliac artery (DCIA) and the lateral thoracic artery (LTA) (14). The flap and its neighboring skinfold tissue were then sutured into a skinfold chamber, which consisted of two symmetrical titanium frames (28). This was done in such a fashion that the observation window, incorporated in one of the frames, allowed direct view onto the muscle and subcutaneous tissue of the elevated flap.…”
Section: Methodsmentioning
confidence: 99%