Experimental Oesophagostomum dentatum infections in the pig: Worm populations resulting from trickle infections with three dose levels of larvae

Roepstorff, Allan; Bjørn, H.; Nansen, P.; Barnes, Elizabeth H; Çhristensen, C. M.

doi:10.1016/0020-7519(96)00006-9

Cited by 50 publications

(27 citation statements)

References 28 publications

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“…This is in accordance with the findings of Permin et al (1997a, b) that chickens fed different doses of embryonated A. galli eggs harboured equal worm burdens 8 week later. Christensen et al (1995) found that pigs infected once with varying doses of Oesophagostomum dentatum resulted in equal worm burdens on day 11 post infection, whereas Roepstorff et al (1996) found that pigs trickle infected with O. dentatum showed an increase in the mean worm burdens with the dose rate. Waller et al (1987) did not observe significant differences in worm burdens in lambs kept under low stocking rates compared to high stocking rates.…”

Section: Discussionmentioning

confidence: 97%

Studies onAscaridia galliin chickens kept at different stocking rates

et al. 1998

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The development of Ascaridia galli infections was investigated over a period of 378 days in parasite naïve chicken flocks ('permanent flocks') kept at different stocking rates and infected by introducing previously infected chickens (seeder birds). The results of this study indicated that differences in stocking rate may lead to different levels of establishment of A. galli infections in chickens. Furthermore, significantly higher weight gains were seen in the medium stocking rate (MSR) group than in either the low stocking rate (LSR) or the high stocking rate (HSR) groups. Periodically, the egg excretion was significantly different between the groups. Although not significant for the whole period, the general trend was that the egg excretion was lower in the MSR group than the LSR and HSR groups. The mean worm burdens in the permanent flocks at the end of the experiment were not significantly different from each other. To estimate the availability of infective eggs in the houses and pens, tracer animals were introduced into each of the permanent flocks every month. Information derived from tracer animals did not confirm an effect of stocking rate on the availability of infective eggs in the environment, although there was a trend towards higher mean worm burdens in the tracers from the MSR group.

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Section: Discussionmentioning

confidence: 97%

Studies onAscaridia galliin chickens kept at different stocking rates

et al. 1998

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“…Dierent authors give dierent explanations, proposing that it might be governed by the age of the pigs (Supperer 1956), the species composition of the worm isolates (Tas 1968;Nickel and Haupt 1969), the dose levels (Christensen et al 1995Roepstor et al 1996), or parasite species interactions . The age of experimental pigs does not appear to determine the duration of the prepatent period, according to experimental infections with our laboratory isolates (Bjùrn and Roepstor, unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Prepatent periods of different Oesophagostomum spp. isolates in experimentally infected pigs

Talvik¹,

Çhristensen²,

Joachim³

et al. 1997

Parasitology Research

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To define prepatent periods of different Oesophagostomum spp. isolates we carried out two separate experiments, one using two monospecific laboratory isolates and another using laboratory isolates as well as isolates obtained from pig herds having different management systems and with different anthelmintic treatment histories. Pigs were inoculated with 1,000-2,000 infective larvae. Fecal samples were collected daily beginning on days 15 and 16 postinoculation (p.i.). Fecal cultures were set up at different times to yield larvae that could be identified by DNA analyses. All pigs started to excrete eggs on days 18-24 p.i. The mean prepatent period was 20.2 +/- 1.4 days, with no significant difference being observed between species and isolates. Prepatent periods of 17-19 days were found for the monospecific laboratory isolates of O. dentatum and O. quadrispinulatum. These findings conflict with parasitology textbooks; therefore, suggestions as to the possible reasons for the observed short prepatent periods are given.

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“…Dobson et al (1990) made a cohort study using levamisole resistant and susceptible strains of Trichostrongylus colubriformis, whereas Roepstorff et al (1996) used pyrantel resistant and susceptible strains of Oesophagostomum dentatum in their study. But this approach to cohort studies results only in two different cohorts and depends on availability of solidly resistant strains.…”

Section: Discussionmentioning

confidence: 99%

Molecular and parasitological tools for the study ofAscaridia gallipopulation dynamics in chickens

et al. 2010

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Experiments were first conducted to compare and evaluate different methods of Ascaridia galli larval recovery from the chicken intestine. The number of larvae recovered from the intestinal wall of chickens infected with 1000 embryonated A. galli eggs and killed 15 days post infection (p.i.) by three methods (ethylenediamine tetraacetic acid [EDTA], pepsin digestion and scraping) were compared. The EDTA and pepsin digestion were found to be the most efficient methods with no significant difference (P!0.05) in the number of recovered larvae between the two. Subsequently, three different A. galli cohorts were established using the polymerase chain reaction-linked restriction fragment length polymorphism (PCR-RFLP) technique. A 533-bp long region of the cytochrome c oxidase subunit 1 gene of the mitochondrial DNA was targeted and 22 A. galli females were allocated to three different haplotypes. The four females with the highest embryonation rate from each haplotype group (total 12 females) were selected and used to inoculate each of 12 chickens with a dose of 1000 embryonated eggs. The chickens were killed 15 days p.i. and A. galli larvae were recovered from the small intestinal wall by the EDTA method and by sieving the lumen content on a 90 mm sieve. DNA of 40 larvae from each of the three different haplotypes was extracted using a worm lysis buffer, and PCR-RFLP analysis of these larvae revealed the same haplotype as that of their maternal parent. The identification of distinguishable cohorts may be a powerful tool in population studies of parasite turnover within the animal host.

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Experimental Oesophagostomum dentatum infections in the pig: Worm populations resulting from trickle infections with three dose levels of larvae

Cited by 50 publications

References 28 publications

Studies onAscaridia galliin chickens kept at different stocking rates

Studies onAscaridia galliin chickens kept at different stocking rates

Prepatent periods of different Oesophagostomum spp. isolates in experimentally infected pigs

Molecular and parasitological tools for the study ofAscaridia gallipopulation dynamics in chickens

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