Experimental ovine salmonellosis (Salmonella Abortusovis): Pathogenesis and vaccination

Pardon, Pierre; Sanchis, R.; Marly, J; Lantier, Frédéric; Guilloteau, Laurence; Buzoni‐Gatel, Dominique; Oswald, Isabelle P.; Pépin, Michel; Kaeffer, Bertrand; Berthon, Patricia; Popoff, M.Y.

doi:10.1016/0923-2508(90)90134-c

Cited by 35 publications

(19 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transmission occurs either by contact between infected and susceptible individuals or by contaminating feed and pastures (Pardon et al 1988;Maurin and Raoult 1999). After contamination, these bacteria colonize the uterus during the Wrst reproductive event and lead to either abortion or birth of infected kids, which generally die within 3 or 4 weeks (Pardon et al 1990;Maurin and Raoult 1999;Longbottom and Coulter 2003). Coxiella and Chlamydophila are generalist pathogens (Maurin and Raoult 1999;Longbottom and Coulter 2003), unlike Salmonella Abortusovis, which is allegedly restricted to sheep (Cagiola et al 2007) although infection in goats has been reported (Sanchis and Cornille 1980).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Diseases and reproductive success in a wild mammal: example in the alpine chamois

et al. 2008

View full text Add to dashboard Cite

Density-dependent and climatic factors affect reproduction and dynamics of wild ungulates. Parasites can also decrease reproductive success through either a direct abortive effect or a negative impact on host growth and body condition. However, few studies have investigated the effect of parasitism on fecundity of ungulates in natural conditions. We studied three bacterial infections caused by Salmonella enterica serovar Abortusovis, Chlamydophila abortus and Coxiella burnetii. These bacteria are leading causes of reproductive failure in sheep, goat and cattle, which raises the question of their influence on population dynamics of wild ungulates. A long-term study of demography and epidemiology of an alpine chamois (Rupicapra rupicapra, L.) population (Les Bauges Reserve, France) and a generalized linear modeling approach were used to analyze the reproductive success of chamois according to population density, weather conditions and the prevalence of antibodies against the three bacteria in females. This approach enabled us to identify the confounding effect of weather and parasitism on fecundity in a natural population. After accounting for density, the prevalence of antibodies against the three bacteria explained 36% of the annual variation in reproductive success, and weather conditions explained an additional 31%. This study was, to our knowledge, the first to compare the decrease in fecundity due to bacterial infections and weather conditions in a population of wild mountain ungulates.

show abstract

Section: Introductionmentioning

confidence: 98%

“…Salmonella enterica serovar Abortusovis (hereafter Salmonella Abortusovis) is the main cause of ovine abortion in Europe and western Asia (Pardon et al 1990). Chlamydophila abortus (formerly Chlamydia psittaci) is one of the most important causes of ovine and caprine abortions worldwide (Longbottom and Coulter 2003).…”

Section: Introductionmentioning

confidence: 99%

Diseases and reproductive success in a wild mammal: example in the alpine chamois

et al. 2008

View full text Add to dashboard Cite

show abstract

“…SS44 is an isolate from sheep originating from Sardinia, Italy, that has been used extensively in virulence studies (27,31,34). 15/5 is a French isolate that has been used to analyze the immune response of sheep to serovar Abortusovis (9,14,22,23). Additional S. enterica strains analyzed by PCR and Southern blot hybridization were from our personal collection of clinical isolates.…”

Section: Methodsmentioning

confidence: 99%

Transposition of the Heat-Stable ToxinastAGene into a Gifsy-2-Related Prophage ofSalmonella entericaSerovar Abortusovis

et al. 2004

View full text Add to dashboard Cite

The horizontal transfer and acquisition of virulence genes via mobile genetic elements have been a major driving force in the evolution of Salmonella pathogenicity. Serovars of Salmonella enterica carry variable assortments of phage-encoded virulence genes, suggesting that temperate phages play a pivotal role in this process. Epidemic isolates of S. enterica serovar Typhimurium are consistently lysogenic for two lambdoid phages, Gifsy-1 and Gifsy-2, carrying known virulence genes. Other serovars of S. enterica, including serovars Dublin, Gallinarum, Enteritidis, and Hadar, carry distinct prophages with similarity to the Gifsy phages. In this study, we analyzed Gifsy-related loci from S. enterica serovar Abortusovis, a pathogen associated exclusively with ovine infection. A cryptic prophage, closely related to serovar Typhimurium phage Gifsy-2, was identified. This element, named Gifsy-2AO, was shown to contribute to serovar Abortusovis systemic infection in lambs. Sequence analysis of the prophage b region showed a large deletion which covers genes encoding phage tail fiber proteins and putative virulence factors, including type III secreted effector protein SseI (GtgB, SrfH). This deletion was identified in most of the serovar Abortusovis isolates tested and might be dependent on the replicative transposition of an adjacent insertion sequence, IS1414, previously identified in pathogenic Escherichia coli strains. IS1414 encodes heat-stable toxin EAST1 (astA) and showed multiple genomic copies in isolates of serovar Abortusovis. To our knowledge, this is the first evidence of intergeneric transfer of virulence genes via insertion sequence elements in Salmonella. The acquisition of IS1414 (EAST1) and its frequent transposition within the chromosome might improve the fitness of serovar Abortusovis within its narrow ecological niche.

show abstract

“…Importantly, results from the mouse model have been con¢rmed in ovine infection [8]. In the present study, our objectives were: (1) to evaluate the invasion of Peyer's patches, mesenteric lymph nodes, and spleen by S. abortusovis as compared to S. typhimurium in a murine model of oral infection ; and (2) to examine the e¡ect of S. abortusovis plasmid curing and mutagenesis after oral and intraperitoneal infection of BALB/c mice.…”

Section: Introductionmentioning

confidence: 99%

Role of the Salmonella abortusovis virulence plasmid in the infection of BALB/c mice

Uzzau¹

2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

Following oral inoculation of BALB/c mice, Salmonella abortusovis strain SS44 was recovered in lower numbers from the Peyer's patches and mesenteric lymph nodes compared with S. typhimurium strain SL1344, whereas splenic infections were equivalent between the two serovars. SS44 was cured of its virulence plasmid or subjected to mutagenesis of the spv genes, and the Spv 3 derivatives were tested for virulence in mice. Plasmid-cured S. abortusovis SU40 retained virulence in BALB/c mice when inoculated intraperitoneally. On the other hand, mice infected orally with SU40 had greatly reduced splenic infection compared to those infected with wild-type SS44. Similar results were obtained after Tn5 insertion mutagenesis of the spvR gene or deletion of the spvABCD locus. These results suggest that in the gutassociated lymphoid tissues S. abortusovis may replicate less than S. typhimurium and that the S. abortusovis virulence plasmid primarily affects systemic infection after oral inoculation but not after intraperitoneal administration in the mouse model. ß 2000 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

show abstract

Experimental ovine salmonellosis (Salmonella Abortusovis): Pathogenesis and vaccination

Cited by 35 publications

References 7 publications

Diseases and reproductive success in a wild mammal: example in the alpine chamois

Diseases and reproductive success in a wild mammal: example in the alpine chamois

Transposition of the Heat-Stable ToxinastAGene into a Gifsy-2-Related Prophage ofSalmonella entericaSerovar Abortusovis

Role of the Salmonella abortusovis virulence plasmid in the infection of BALB/c mice

Contact Info

Product

Resources

About