Experimental periodontitis in <i>Msx2</i> mutant mice induces alveolar bone necrosis

Korah, Linda; Amri, Nawel; Bugueno, Isaac Maximiliano; Hotton, Dominique; Tenenbaum, Henri; Huck, Olivier; Berdal, Ariane; Davideau, Jean‐Luc

doi:10.1002/jper.16-0435

Cited by 11 publications

(9 citation statements)

References 46 publications

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“…In a previous report, Towler DA et al detected the expression of Msx2 in ovoid pre-odontoblasts (Bidder et al, 1998). It is involved in many molecular pathways controlling mineralized tissue homeostasis, such as the Wnt/sclerostin pathway (Korah et al, 2019). In our study, the expression of Col-10 was upregulated nearly 4.3-fold (Figure 4).…”

Section: Discussionsupporting

confidence: 63%

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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Native dental pulp extracellular matrix (DPEM) has proven to be an effective biomaterial for dental pulp regeneration. However, as a significant extracellular matrix glycoprotein, partial laminins were lost during the decellularization process, which were essential for odontoblast differentiation. Thereby, this study investigated the feasibility of LN supplementation to improve the surface of DPEM for odontoblast layer regeneration. The influences of laminin on cell adhesion and odontogenic differentiation were evaluated in vitro. Then, we fabricated laminin-modified DPEM based on the physical coating strategy and observed the location and persistency of laminin coating by immunofluorescent staining. Finally, laminin-modified DPEM combined with treated dentin matrix (TDM) was transplanted in orthotopic jaw bone of beagles (n = 3) to assess the effect of LNs on dental pulp tissue regeneration. The in vitro results showed that laminins could improve the adhesion of dental pulp stem cells (DPSCs) and promoted DPSCs toward odontogenic differentiation. Continuous odontoblastic layer-like structure was observed in laminin-modified DPEM group, expressing the markers for odontoblastogenesis, dentine matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP). Overall, these studies demonstrate that the supplementation of laminins to DPEM contributes to the odontogenic differentiation of cells and to the formation of odontoblast layer in dental pulp regeneration.

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Section: Discussionsupporting

confidence: 63%

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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“…Many studies have used the periodontitis model by placing ligatures inoculated with P. gingivalis around the teeth of the mice to induce massive local accumulation of periodontal pathogens [10,[177][178][179][180][181][182][183][184][185][186][187][188]190,191,194,195]. In a representative study using this model, Li and Amar [188] provided evidence that the mouse periodontal model involving a ligature inoculated with P. gingivalis exhibited a more adequate inflammatory response and greater periodontal tissue breakdown than the control group, which induced periodontitis by ligature alone.…”

Section: Periodontitis Model Induced By Ligature Inoculated With P Gingivalismentioning

confidence: 99%

Application of Ligature-Induced Periodontitis in Mice to Explore the Molecular Mechanism of Periodontal Disease

Lin

Niimi

Ohsugi

et al. 2021

IJMS

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Periodontitis is an inflammatory disease characterized by the destruction of the periodontium. In the last decade, a new murine model of periodontitis has been widely used to simulate alveolar bone resorption and periodontal soft tissue destruction by ligation. Typically, 3-0 to 9-0 silks are selected for ligation around the molars in mice, and significant bone loss and inflammatory infiltration are observed within a week. The ligature-maintained period can vary according to specific aims. We reviewed the findings on the interaction of systemic diseases with periodontitis, periodontal tissue destruction, the immunological and bacteriological responses, and new treatments. In these studies, the activation of osteoclasts, upregulation of pro-inflammatory factors, and excessive immune response have been considered as major factors in periodontal disruption. Multiple genes identified in periodontal tissues partly reflect the complexity of the pathogenesis of periodontitis. The effects of novel treatment methods on periodontitis have also been evaluated in a ligature-induced periodontitis model in mice. This model cannot completely represent all aspects of periodontitis in humans but is considered an effective method for the exploration of its mechanisms. Through this review, we aimed to provide evidence and enlightenment for future studies planning to use this model.

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“…MSX2 functional deletion mutation in a mouse model caused impaired enamel formation 21 . Alveolar bone necrosis is induced by experimental periodontitis in Msx2 mutant mice 22 . It can be seen that MSX2 plays an important role in the development of enamel and alveolar bone.…”

Section: Discussionmentioning

confidence: 99%

The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ

Zhang

Zhao

et al. 2021

J Cellular Molecular Medi

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Muscle segment homeobox 2 (MSX2) has been confirmed to be involved in the regulation of early tooth development. However, the role of MSX2 has not been fully elucidated in enamel development. To research the functions of MSX2 in enamel formation, we used a Msx2−/− (KO) mouse model with no full Msx2 gene. In the present study, the dental appearance and enamel microstructure were detected by scanning electron microscopy and micro‐computed tomography. The results showed that the absence of Msx2 resulted in enamel defects, leading to severe tooth wear in KO mice. To further investigate the mechanism behind the phenotype, we performed detailed histological analyses of the enamel organ in KO mice. We discovered that ameloblasts without Msx2 could secrete a small amount of enamel matrix protein in the early stage. However, the enamel epithelium occurred squamous epithelial hyperplasia and partial keratinization in the enamel organ during subsequent developmental stages. Ameloblasts depolarized and underwent pyroptosis. Overall, during the development of enamel, MSX2 affects the formation of enamel by regulating the function of epithelial cells in the enamel organ.

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Experimental periodontitis in Msx2 mutant mice induces alveolar bone necrosis

Cited by 11 publications

References 46 publications

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Application of Ligature-Induced Periodontitis in Mice to Explore the Molecular Mechanism of Periodontal Disease

The absence of muscle segment homeobox 2 leads to the pyroptosis of ameloblasts by inducing squamous epithelial hyperplasia in the enamel organ

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