Experimental pulmonary fat embolism induced by injection of triolein in rats

Inoue, Hiromasa; Hanagama, Masakazu; Kamiya, Munechika; Shinone, Kotaro; Nata, Masayuki

doi:10.1016/j.legalmed.2007.07.001

Cited by 10 publications

(11 citation statements)

References 13 publications

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“…This early mortality rate (36%) was consistent with previously published reports using triolein in rat models of fat embolization. 7,11 After the early phase in which the animals showed less activity and were obtunded for a few hours, they seemed to recover without any obvious differences from the controls as reported previously. 7 After the initial period on day 1, the gross behavior of the trioleintreated animals could not be distinguished from that of the controls.…”

Section: Growth and Behaviorsupporting

confidence: 65%

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Persistent and progressive pulmonary fibrotic changes in a model of fat embolism

Poisner¹,

Adler²,

Uhal³

et al. 2012

Journal of Trauma and Acute Care Surgery

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Section: Growth and Behaviorsupporting

confidence: 65%

“…The triolein dose was selected based on our previous study 7 and studies on anesthetized rats. 11 Rats received ad libitum access to food and water. Clinical observations were made at various time points before and after injection.…”

Section: Methodsmentioning

confidence: 99%

Persistent and progressive pulmonary fibrotic changes in a model of fat embolism

Poisner¹,

Adler²,

Uhal³

et al. 2012

Journal of Trauma and Acute Care Surgery

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“…A control group of 13 rats received 0.2 ml saline. The dose of triolein was selected based on previous work on its distribution in unanesthetized rats,19 and also studies on anesthetized animals 22. Rats were given ad libitum access to food and water.…”

Section: Methodsmentioning

confidence: 99%

“…The following study was undertaken to better understand the time‐course of the pathological response to FE. This report describes the histopathological changes caused by FE in the rat lung, over a period of 11 days, using a previously described experimental model 19, 22…”

mentioning

confidence: 99%

Fat embolism: Evolution of histopathological changes in the rat lung

McIff

Poisner

Herndon

et al. 2009

Journal Orthopaedic Research

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The pathophysiology of Fat Embolism Syndrome (FES) is poorly understood and subject to some controversy. Evaluation of the evolution of histological changes in the lungs of patients with FES is impractical. The current theories of FES were established through acute clinical observations and acute animal experiments, but sequential changes in the histology of lungs over a prolonged period have not been made. The progressive effects of fat embolization of the lungs were examined in a rat model over a period of 11 days. Triolein, a major bone marrow fat, was administered to conscious Sprague-Dawley rats via the caudal vein. Rats were euthanized at 24, 48, 96 h, and 11 days, but some died within a few hours. Histomorphometric evaluations of lung tissue were made, including stains for fat, collagen, and smooth muscle actin. Arterial and arteriolar patency decreased progressively up to 96 h, but returned toward normal after 11 days. A striking finding was the very early presence of inflammation and fibrosis after only several hours, persisting up to 11 days. The results of this study provide evidence of both very early and prolonged changes due to fat embolization. ß

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“…Our previous studies revealed that antemortem heat exposure could develop pulmonary fat embolization [10,11]. Moreover, our previous experiment showed the possibility that, when fat is just injected into a vein without other treatments, the amount of fat required to result in ALI would be more than the amount of fat which can clinically lead to ALI [12]. Thus, we hypothesized that the development of ARDS due to pulmonary fat embolization might be affected by the severity of preconditions which induce pulmonary fat embolization.…”

Section: Introductionmentioning

confidence: 99%