Experimental rat model for alcohol‐induced osteonecrosis of the femoral head

Okazaki, Shunichiro; Nagoya, Satoshi; Tateda, Kenji; Katada, Ryuichi; Mizuo, Keisuke; Watanabe, Satoshi; Yamashita, Toshihiko; Matsumoto, Hiroshi

doi:10.1111/iep.12035

Cited by 36 publications

(42 citation statements)

References 35 publications

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“…To investigate the effects of cordycepin on ethanol-induced ONFH, the rat model of ONFH was established by a Lieber-Decarli ethanol-containing liquid diet [23, 24], followed by intraperitoneal injection of cordycepin or an equal volume of saline as control. Six weeks after corresponding treatment, micro-CT scanning was performed for evaluation and quantification of bone tissues of the femoral head.…”

Section: Resultsmentioning

confidence: 99%

“…We identified typical osteonecrotic lesions of the femoral head in 7/10 rats in the AL group, as diffuse empty lacunae present in bone trabeculae, surrounded by bone marrow cell necrosis [24-26]. After six weeks of corresponding treatment, the subchondral area of femoral heads in the AL group (7/10 rats) were found occupied by empty lacunae with surrounding bone marrow cell necrosis in H&E images (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Alcohol-induced ONFH was induced by a Lieber-Decarli alcohol-containing diet [24]. The in vivo results revealed that cordycepin reduced pathological changes including the decrease in subchondral trabecular bone, diffuse empty lacunae in trabeculae bone and surrounding bone marrow necrosis in typical alcohol-induced ONFH [24].…”

Section: Discussionmentioning

confidence: 99%

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The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

Chen¹,

Zhu²,

Xu³

et al. 2017

Cell Physiol Biochem

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Background: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role. Cordycepin benefits bone metabolism; however, there has been no study to determine its effect on osteonecrosis. Methods: Human bone mesenchymal stem cells (hBMSCs) were identified by multi-lineage differentiation. Alkaline phosphatase (ALP) activity, RT-PCR, western blots, immunofluorescent assay and Alizarin red staining of BMSCs were evaluated. A rat model of alcohol-induced ONFH was established to investigate the protective role of cordycepin against ethanol. Hematoxylin & eosin (H&E) staining and micro-computerized tomography (micro-CT) were performed to observe ONFH. Apoptosis was assessed by TdT-mediated dUTP nick end labeling (TUNEL). Immunohistochemical staining was carried out to detect OCN and COL1. Results: Ethanol significantly suppressed ALP activity, decreased gene expression of OCN and BMP2, lowered levels of RUNX2 protein, and reduced immunofluorescence staining of OCN and COL1 and calcium formation of hBMSCs. However, these inhibitory effects were attenuated by cordycepin co-treatment at concentrations of 1 and 10 µg/mL Moreover, it was revealed that the osteo-protective effect of cordycepin was associated with modulation of the Wnt/β-catenin pathway. In vivo, by micro-CT, TUNEL and immunohistochemical staining of OCN and COL1, we found that cordycepin administration prevented alcohol-induced ONFH. Conclusion: Cordycepin treatment to enhance osteogenesis may be considered a potential therapeutic approach to prevent the development of alcohol-induced ONFH.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

Chen¹,

Zhu²,

Xu³

et al. 2017

Cell Physiol Biochem

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“…Osteonecrosis of the femoral head (ONFH) is a painful disorder of the hip that affects patients with various risk factors, such as glucocorticoid use, alcohol abuse, trauma, and autoimmune diseases (1)(2)(3) . The progress of joint destruction in ONFH can lead to hip arthritis, which seriously affects the patient's health.…”

Section: Introductionmentioning

confidence: 99%

Platelet-rich plasma combined with core decompression and allogeneic fibula rod support for the treatment of osteonecrosis of the femoral head (Ficat stage II): a short-term follow-up

2017

Clin Surg Res Commun

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Platelet-rich plasma combined with core decompression and allogeneic fibula rod support for the treatment of osteonecrosis of the femoral head (Ficat stage II): a short-term follow-up Clin Surg Res Commun 2017; 1: 35-40 35 Biao-fang Wei et al AbstractBackground: The objective of this study was to investigate the potency of platelet-rich plasma (PRP) combined with core decompression and allogeneic fibula rod support for the treatment of osteonecrosis of the femoral head (ONFH, Ficat stage II).Methods: This was a retrospective study of clinical data of patients who were admitted to our hospital for treatment of ONFH from July 2014 to June 2015. In PRP group, patients (9 cases) were treated with plateletrich plasma combined with core decompression and allogeneic fibula rod support. In control group, patients (7 cases) were treated with core decompression and allogeneic fibula rod support only. The time from onset of symptoms to surgery was 3-6 months (mean: 4.5 months). Harris hip scores before and after surgery were recorded (> 90, excellent; 75-90, good; 60-74, poor; and < 60, bad). An imaging study was performed 1 and 3 months after surgery.Results: In the PRP group, the Harris scores increased from 67.82 ± 9.61 to 88.45 ± 6.02. In the control group, the Harris scores increased from 69.74 ± 8.26 to 87.36 ± 6.17. There were statistically significant differences in pretherapy and post-treatment scores (P < 0.05). Three months postsurgery, the shapes of the femoral heads in both groups were good, with no deformation or collapse. The location of the top of the allograft fibula was perfect (no fibula out). Additionally, the fibula rods began to combine with the surrounding bone after 3 months postsurgery, without infection.Conclusion: PRP can ease hip pain and improve joint function

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“…We previously reported an alcohol-induced ONFH animal model using male rats based on the feeding of an ethanol-containing liquid diet [6]. ONFH developed in male rats fed with 5% ethanol-containing liquid diet from only one-week feeding, and the incidence of ONFH in the 4-week ethanol-fed male rats was significantly higher than in the control group [6].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Males, but Not Females to Developing Femoral Head Osteonecrosis in Response to Alcohol Consumption

et al. 2016

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BackgroundWe previously reported that ethanol-containing liquid diet feeding induces osteonecrosis of the femoral head in male rats. Also, it was reported that a large amount of consumed ethanol and a long-term history of drinking were risk factors for osteonecrosis of the femoral head, and that the frequency of alcohol-induced osteonecrosis of the femoral head in males was much greater than in females. The higher incidence of alcohol-induced osteonecrosis of the femoral head could be due to either higher prevalence of alcohol drinking in males or due to their potential higher sensitivity to alcohol. The aim of the study is to investigate the influence of alcohol consumption and drinking period on the development of osteonecrosis of the femoral head in rats of both sex.MethodsAll the experimental male rats were allocated to the male one-month ethanol drinking group (M1). Female rats were randomly divided into the female one- to five-months ethanol drinking groups (F1-5). All rats were fed a Lieber-DeCarli liquid diet containing 5% ethanol for one to five months.ResultsOne-month feeding with the ethanol-containing liquid diet resulted in the development of osteonecrosis of the femoral head in seven of twenty in the M1 group, but none in the F1 group, although the mean intake of ethanol per body weight in the M1 group was significantly lower than that in the F1 group. Furthermore, long drinking periods with a large amount of ethanol intake in the F2-5 groups did not induce osteonecrosis of the femoral head.ConclusionThe present study shows that lower alcohol consumption over short periods of time that were sufficient to induce osteonecrosis of the femoral head in males had no effect on females. Even with greater alcohol consumption and longer duration, females did not develop osteonecrosis of the femoral head. Therefore, unknown factors related to sex must be responsible for the development of this condition.

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Experimental rat model for alcohol‐induced osteonecrosis of the femoral head

Cited by 36 publications

References 35 publications

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

Platelet-rich plasma combined with core decompression and allogeneic fibula rod support for the treatment of osteonecrosis of the femoral head (Ficat stage II): a short-term follow-up

Susceptibility of Males, but Not Females to Developing Femoral Head Osteonecrosis in Response to Alcohol Consumption

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