Experimental Reinnervation of a Strap Muscle with a Few Roots of the Phrenic Nerve in Rabbits

Marie, Jean‐Paul; Lerosey, Y.; Dehesdin, D.; Jin, Olivier; Tadié, M; Andrieu-Guitrancourt, J

doi:10.1177/000348949910801013

Cited by 29 publications

(15 citation statements)

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“…Muscle force measurement is widely used to assess the mechanical function and contractile properties of a muscle [35–41]. Our studies [28,30–33] and others [23,25,42,43] have demonstrated that force measurement is a reliable technique in determining the degree of functional recovery of a reinnervated muscle. The denervated muscle is characterized by reduced muscle mass, decreased fiber size, increased connective tissue, and reduced metabolic capacity [44,45] which can be reversed by reinnervation [28,33,46].…”

Section: Discussionmentioning

confidence: 90%

Muscle reinnervation with nerve-muscle-endplate band grafting technique: correlation between force recovery and axonal regeneration

Sobotka

2015

Journal of Surgical Research

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Background This study was designed to determine the correlation between functional recovery and the extent of axonal regeneration after muscle reinnervation with our recently developed nerve-muscle-endplate band grafting (NMEG) technique in a rat model. Materials and methods The right experimentally paralyzed sternomastoid (SM) muscle by nerve transection was immediately reinnervated with a NMEG pedicle harvested from a neighboring sternohyoid (SH) muscle. The NMEG pedicle contained a muscle block (6x6x3 mm), a donor nerve branch with nerve terminals and a motor endplate band. Three months after surgery, the tetanic force of the SM muscle was measured and the regenerated axons in the muscle were detected using neurofilament immunohistochemistry. Results The results showed that the maximal tetanic force (a measure of muscle functional recovery) of the NMEG reinnervated SM muscle reached up to 66.0% of the normal control. The wet weight of the reinnervated SM muscle (a measure of muscle mass recovery) was 87.2% of the control. The area fraction of the regenerating axons visualized with neurofilament staining within the NMEG reinnervated SM muscle (a measure of muscle reinnervation) was 42.3%. A positive correlation was revealed between the extent of muscle reinnervation and maximal muscle force. Conclusions Our newly developed NMEG technique results in satisfactory functional outcomes and nerve regeneration. Further improvement in the functional recovery after NMEG reinnervation, could be achieved by refining the surgical procedure and creating an ideal environment that favors axon-endplate connections and accelerates axonal growth and sprouting.

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Section: Discussionmentioning

confidence: 90%

Muscle reinnervation with nerve-muscle-endplate band grafting technique: correlation between force recovery and axonal regeneration

Sobotka

2015

Journal of Surgical Research

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“…A number of approaches are available for evaluating the extent of regenerating axons in an anastomosed nerve (McDonald et al,2006) or a reinnervated muscle (Bisby and Tetzlaff,1992), and newly formed MEPs in the reinnervated muscle (Pachter and Eberstein,1991; Walter et al,1996). In this regard, retrograde HRP labeling is frequently used to determine the source of innervation of a given reinnervated muscle (Neal et al,1981; Anonsen et al,1985; Meikle et al,1987; Marie et al,1999; Paniello et al,2001) and changes in the number of the labeled motoneurons after nerve injury and muscle reinnervation (Thomander,1984; Aldskogius and Thomander,1986; Brown and Hardman,1987; Fujito et al,1989; Mallonga and Ontell,1991; Pollin et al,1991; Scaribrick et al, 1992; Ito et al,1994).…”

Section: Discussionmentioning

confidence: 99%

“…The location and frequency of the HRP‐labeled motoneurons innervating a given muscle are two parameters commonly used in the reinnervation studies (Pollin et al,1991; Scaribrick et al, 1992; Ito et al,1994; Marie et al,1999; Paniello et al,2001). For example, a denervated SM muscle can be either self‐reinnervated by its original nerve or foreign‐reinnervation by a nerve supplying a cervical strap muscle.…”

Section: Discussionmentioning

confidence: 99%

“…For example, SM is often employed as muscle and myocutaneous flap for reconstruction of oral cavity and facial defects (Charles et al,1987; Alvi and Stegnjajic,1994; Hamilton et al,2006; Kumar et al,2009), and for esophageal perforation repair and esophagoplasty (Frimpong‐Boateng,1994; Navarro et al,2005; Ananthakrishnan et al,2007; Benazzo et al,2008; Parell and Cassisi,2008). Cervical strap muscles (Neal et al,1981; Anonsen et al,1985; Meikle et al,1987; Marie et al,1999) including SM (Kaser and Muntener,1989) are also good candidates for head and neck muscle reinnervation. For instance, nerve‐muscle‐pedicle (NMP) transfer has been used to treat laryngeal and facial paralysis (Tucker1976,1979; Crumley,1982; Meikle et al,1987; Goding et al,1989).…”

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confidence: 99%

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Locations of the Motor Endplate Band and Motoneurons Innervating the Sternomastoid Muscle in the Rat

Zhang

et al. 2010

The Anatomical Record

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Sternocleidomastoid (SCM) is a long muscle with two bellies, sternomastoid (SM) and cleidomastoid (CM) in the lateral side of the neck. It has been widely used as muscle and myocutaneous flap for reconstruction of oral cavity and facial defects and as a candidate for reinnervation studies. Therefore, exact neuroanatomy of the SCM is critical for guiding reinnervation procedures. In this study, SM in rats were investigated to document banding pattern of motor endplates (MEPs) using whole-mount acetylcholinesterase (AChE) staining and to determine locations of the motoneurons innervating the muscle using retrograde horseradish peroxidase (HRP) tracing technique. The results showed that the MEPs in the SM and CM were organized into a single band which was located in the middle portion of the muscle. After HRP injections into the MEP band of the SM, ipsilaterally labeled motoneurons were identified in the caudal medulla oblongata (MO), C1, and C2. The SM motoneurons were found to form a single column in lower MO and dorsomedial (DM) nucleus in C1. In contrast, the labeled SM motoneurons in C2 formed either one (DM nucleus), two [DM and ventrolateral (VL) nuclei], or three [DM, VL, and ventromedial (VM)] columns. These findings are important not only for understanding the neural control of the muscle but also for evaluating the success rate of a given reinnervation procedure when the SM is chosen as a target muscle. Anat Rec, 294:295-304, 2011. V V C 2010 Wiley-Liss, Inc.

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“…15 Rabbits were chosen for these experiments. Their phrenic nerves are mainly constituted by 3 cervical roots, as is the case in dogs and humans.…”

Section: Introductionmentioning

confidence: 99%

Cervical Anatomy of Phrenic Nerve Roots in the Rabbit

Marie¹,

Lerosey²,

Tadié³

et al. 1999

Ann Otol Rhinol Laryngol

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The cervical anatomy of the different nerve contributions that constitute the phrenic nerve (phrenic nerve roots and accessory phrenic nerve) were studied in rabbits. In 55 dissections, 6 main root arrangement types were observed. The roots that issued from the fourth and fifth cervical nerves (C4 and C5 roots) were constant. The C4 root was either short or long. The C6 root was at times absent, or sometimes double. An accessory phrenic nerve was present in 43% of the right and 28% of the left dissections. The distribution of the phrenic nerve roots often displayed left-right asymmetry. We conclude that a better knowledge of the cervical anatomy of the phrenic nerve is useful both in physiological studies involving diaphragm denervation and in experimental laryngeal reinnervation.

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Experimental Reinnervation of a Strap Muscle with a Few Roots of the Phrenic Nerve in Rabbits

Cited by 29 publications

References 29 publications

Muscle reinnervation with nerve-muscle-endplate band grafting technique: correlation between force recovery and axonal regeneration

Muscle reinnervation with nerve-muscle-endplate band grafting technique: correlation between force recovery and axonal regeneration

Locations of the Motor Endplate Band and Motoneurons Innervating the Sternomastoid Muscle in the Rat

Cervical Anatomy of Phrenic Nerve Roots in the Rabbit

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