The purpose of this study is to investigate the impacts of diesel-biodiesel-alcohol blends on the combustion, performance and emissions characteristics of a single-cylinder diesel engine. Tests were conducted at different engine speeds of 1750, 2250, 2750 and 3250 rpm and under full load. In this study, different fuels [called as reference diesel (D100), 20 vol% cottonseed methyl ester (D80C20), 10 vol% ethanol (D90E10) and finally the ternary type of their derivations (D70C20E10)], were used. The experimental results showed that the highest reduction values were observed on CO emission by 42%, 30% and 8% for the D90E10, D70C20E10 and D80C20 fuels, respectively. These reductions for HC emission were achieved as 40%, 31% and 23% for the D90E10, D70C20E10 and D80C20, respectively. On the other hand, the reductions of NO x and CO 2 emissions were not sharp and varied between 2-7%. Besides the reductions on the exhaust emissions, biodiesel-ethanol blend presented better results in terms of HRR max and CP max than using biodiesel alone. Additionally, ignition delay of the biodiesel blends was longer than that of D100 fuel owing to their low cetane numbers. Combustion duration was shortened with the increment in engine speed because the turbulence increased in the combustion chamber at high engine speed. This case also improved the homogeneity of test fuels and increased the quality of the combustion process. As a consequence, this paper clearly reported that it is possible to achieve fewer emissions, the highest CP max values with the presence of ethanol in biodiesel fuels rather than using biodiesel alone for diesel engines.