Experimental research The effect of esculentoside A on lupus nephritis-prone BXSB mice

Ma, Hualin; Zhang, Xianggui; Zhang, Xinzhou; Yang, Diya; Meng, Ling‐Guo; Zhang, Yi; Zhou, Shao-Lai

doi:10.5114/aoms.2012.31439

Cited by 15 publications

(15 citation statements)

References 24 publications

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“… [9] . EsA has the effect of modulating immune response, cellular proliferation and apoptosis as well as anti-inflammatory effects in acute and chronic experimental models [10] , [11] . Ju DW et al have demonstrated that EsA has the ability to inhibit pro-inflammatory cytokine production such as TNF-α, IL-1β, IL-2, IL-6 and prostaglandin E2 in several cell types [12] , [13] .…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Esculentoside A on Experimental Acute Liver Injury in Mice

et al. 2014

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Inflammatory response and oxidative stress are considered to play an important role in the development of acute liver injury induced by carbon tetrachloride (CCl4) and galactosamine (GalN)/lipopolysaccharides (LPS). Esculentoside A (EsA), isolated from the Chinese herb phytolacca esculenta, has the effect of modulating immune response, cell proliferation and apoptosis as well as anti-inflammatory effects. The present study is to evaluate the protective effect of EsA on CCl4 and GalN/LPS-induced acute liver injury. In vitro, CCK-8 assays showed that EsA had no cytotoxicity, while it significantly reduced levels of TNF-α and cell death rate challenged by CCl4. Moreover, EsA treatment up-regulated PPAR-γ expression of LO2 cells and reduced levels of reactive oxygen species (ROS) challenged by CCl4. In vivo, EsA prevented mice from CCl4-induced liver histopathological damage. In addition, levels of AST and ALT were significantly decreased by EsA treatment. Furthermore, the mice treated with EsA had a lower level of TNF-α, Interleukin (IL)-1β and IL-6 in mRNA expression. EsA prevented MDA release and increased GSH-Px activity in liver tissues. Immunohistochemical staining showed that over-expression of F4/80 and CD11b were markedly inhibited by EsA. The western bolt results showed that EsA significantly inhibited CCl4-induced phosphonated IkBalpha (P-IκB) and ERK. Furthermore, EsA treatment also alleviated GalN/LPS-induced acute liver injury on liver enzyme and histopathological damage. Unfortunately, our results exhibited that EsA had no effects on CCl4-induced hepatocyte apoptosis which were showed by TUNEL staining and Bax, Caspase-3 and cleaved Caspase-3 expression. Our results proved that EsA treatment attenuated CCl4 and GalN/LPS-induced acute liver injury in mice and its protective effects might be involved in inhibiting inflammatory response and oxidative stress, but not apoptosis with its underlying mechanism associated with PPAR-γ, NF-κB and ERK signal pathways.

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Section: Introductionmentioning

confidence: 99%

The Protective Effect of Esculentoside A on Experimental Acute Liver Injury in Mice

et al. 2014

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“…Esculentoside A (EsA) is a triterpene saponin derived from the root of a perennial herb, Phytolacca esculenta . EsA has activity in regulating immune response, cell proliferation and apoptosis, and possesses antiinflammation activity and protective potential of liver damage against acetaminophen toxicity in previous investigations (Hu et al, ; Ma et al, ; Wang et al, ; Zhang et al, ; Zhong et al, ). EsA inhibited proinflammatory factors and inflammatory cytokine production in vitro and in vivo (Ma et al, ; Wang et al, ; Yang, Wang, Yu, Zhu, & Xu, ; Zhang et al, ; Zhong et al, ); EsA exerts antiinflammatory activity by targeting ribosomal protein S3a and impairing its signaling transduction (Li et al, ).…”

Section: Introductionmentioning

confidence: 95%

“…EsA has activity in regulating immune response, cell proliferation and apoptosis, and possesses antiinflammation activity and protective potential of liver damage against acetaminophen toxicity in previous investigations (Hu et al, ; Ma et al, ; Wang et al, ; Zhang et al, ; Zhong et al, ). EsA inhibited proinflammatory factors and inflammatory cytokine production in vitro and in vivo (Ma et al, ; Wang et al, ; Yang, Wang, Yu, Zhu, & Xu, ; Zhang et al, ; Zhong et al, ); EsA exerts antiinflammatory activity by targeting ribosomal protein S3a and impairing its signaling transduction (Li et al, ). After EsA treatment, the production of inflammatory cytokines induced by lipopolysaccharide, such as tumor necrosis factor‐α, interleukin‐1, interleukin‐6 (IL‐6), nitric oxide, and nuclear factor‐κB p65, was decreased significantly, which contributed to the strong antiinflammatory activity of EsA (Chen et al, ; Ju, Zheng, Cao, Fang, & Wang, ; Li et al, ; Yang, Chen, Yu, & Xu, ; Yang et al, ; Zhong et al, ).…”

Section: Introductionmentioning

confidence: 95%

Esculentoside A suppresses breast cancer stem cell growth through stemness attenuation and apoptosis induction by blocking IL‐6/STAT3 signaling pathway

Liu

Dong

Sun

et al. 2018

Phytotherapy Research

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Breast cancer stem cells (CSCs) survive in inflammatory microenvironment, their survival are regulated by inflammatory cytokines and signaling pathways. Esculentoside A (EsA), a triterpene saponin derived from the root of Phytolacca esculenta, possesses antiinflammation effects; but whether it has anticancer activity is unknown. The purpose of this study is to test the inhibitory effect of EsA on the growth of breast CSCs and to elucidate its probable mechanisms of action. The proliferation inhibitory effect of EsA on breast CSCs in vitro were determined by cytotoxicity, mammosphere formation inhibition, apoptotic cell detection assays, and in vivo tumor growth inhibition experiment. The possible molecular mechanisms elucidating the inhibitory effect of EsA on breast CSC growth were examined with western blotting. EsA caused proliferation and mammosphere formation inhibition of breast CSCs; induced breast CSCs apoptotic death; suppressed the growth of tumors generated from breast CSCs significantly; the expressions of stemness proteins including ALDH1A1, Sox2, and Oct4 were downregulated; proapoptotic proteins, Bax and cleaved caspase-3 were upregulated, whereas the antiapoptotic protein Bcl-2 was reduced; IL-6/STAT3 pathway proteins including IL-6, phosphorylated STAT3 (Tyr705), and STAT3 (Ser727) were downregulated significantly in EsA-treated breast CSCs and tumor tissues. EsA inhibited breast CSC growth in vitro and in vivo through stemness attenuation and apoptosis induction by blocking IL-6/STAT3 signaling pathway; it might serve as a novel candidate agent for human breast cancer treatment and/or prevention.

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“…The hybridized probe was detected using an in situ hybridization detection kit (Wuhan Boster Biological Technology, Ltd., Wuhan, China) according to the manufacturer's instructions (13). Briefly, the slides were incubated with blocking reagent for 30 min at 37˚C, and then with biotin-labeled sheep antidigoxigenin for 1 h at 37˚C.…”

Section: Ihc and Ish In The Tissue Microarray (Tma)mentioning

confidence: 99%

Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue

Jiang

Bai

Meng³

et al. 2015

Oncology Letters

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Abstract. Thyroid transcription factor-1 (TTF-1) reportedly possesses oncogenic and suppressive roles within the same tumor type and may play a dual function in the progression of lung cancer. Immunohistochemistry (IHC) and mRNA in situ hybridization (ISH) are commonly used methods for detecting protein or mRNA expression. The present study compared the concordance rate of the two methods in the evaluation of thyroid transcription factor-1 (TTF-1) expression in non-small cell lung carcinoma (NSCLC) using tissue microarray-based IHC and mRNA ISH. TTF-1 protein and mRNA expression levels were examined in 196 cases of NSCLC. The IHC and mRNA ISH agreement was 91.3% (179/196), and near-perfect agreement was observed between the two methods (κ-coefficient, 0.848). There was no significant difference between IHC and mRNA ISH, as analyzed by the McNemar-Bowker test (P=0.219). The present findings proved that IHC is comparable to mRNA ISH for evaluating TTF-1 expression in NSCLC. These two methods can be used to detect TTF-1 expression in future studies.

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Experimental research The effect of esculentoside A on lupus nephritis-prone BXSB mice

Cited by 15 publications

References 24 publications

The Protective Effect of Esculentoside A on Experimental Acute Liver Injury in Mice

The Protective Effect of Esculentoside A on Experimental Acute Liver Injury in Mice

Esculentoside A suppresses breast cancer stem cell growth through stemness attenuation and apoptosis induction by blocking IL‐6/STAT3 signaling pathway

Comparison of immunohistochemistry and mRNA in situ hybridization in detecting thyroid transcription factor-1 expression in non-small cell lung carcinomas tissue

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