Experimental rodent models of chronic prostatitis and evaluation criteria

Wang, Wenlu; Naveed, Muhammad; Abbas, Muhammad; Zhou, Xiaohui

doi:10.1016/j.biopha.2018.10.010

Cited by 32 publications

(22 citation statements)

References 41 publications

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“…The T2 peptide was mixed with CFA and configured into different concentrations of peptide immunoprecipitates. The final concentration of T2 for C57BL/6 mice were 225 μg/ml (Khan et al, ; Zhang et al, ), and SD rats were 50 μg/ml (Ihsan et al, ; Wang et al, ). All animal experiments were approved and conducted under the guidelines of the Committee for Animal Care and Use of the China Pharmaceutical University.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the "start switch" of CP/CPPS was explored for the first time, which provided clues for future treatment of CP/CPPS. In our laboratory, T2 peptide (TRPM8 short peptide with low homology with other TRP family) obtained by software screening and combined with Freund's adjuvant to immunise animals, and the CP/CPPS animal model was successfully prepared (Wang, Naveed, Baig, Abbas, & Xiaohui, ).…”

Section: Introductionmentioning

confidence: 99%

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Morphological reseach on expression of inflammatory mediators in murine models of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by T2 antigen

et al. 2019

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Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common clinical syndrome with unknown aetiology. In this study, we used the T2 peptide in C57BL/6 (B6) mice and Sprague Dawley (SD) rats model during different stages. We sought to understand the role of CD4 + T cells and macrophages in CP/CPPS. A total of 16 B6 mice and 18 SD rats were divided into five groups: B6-naïve (n = 6), B6 model (n = 10), SD-naïve (n = 6), SD-45-day model (n = 6) and SD-56-day model (n = 6). The B6 model group was subcutaneously injected with 0.2 ml of (225μg/ml) T2 peptide on 0 and 14th day and was finally sacrificed on 28th day. The SD-45-and SD-56-day model groups were subcutaneously injected with 1ml of (50 μg/ml) T2 peptide on 0 and 14th day and were finally sacrificed on 45th and 56th day respectively. An equivalent volume of normal saline (NS) solution was injected to the naïve groups and analysed the pain and voiding behaviour. We have calculated the prostate index, H&E staining and immunofluorescence of CD4 + T cells and macrophages (CD68) in each group. T2 peptide immunization in B6 mice and SD rats caused severe prostatitis and cell infiltration, mainly composed of CD4 + T cells and macrophages. The SD-56-day model group showed more severe inflammatory cells infiltration than SD-45-day model group. Moreover, inflammatory cells infiltration and red secretions in B6 model were less than SD model. Expression of CD4 + T cells and macrophages was also consistent with H&E results. These results indicated that different stages of CP/CPPS, inflammatory response, and the inflammation of the rat were stronger than the mouse. Our study suggests that CD4 + T cells and macrophages are key factors in the development of CP/CPPS. K E Y W O R D SCD4 + T cells, CP/CPPS, EAP, macrophages, T2 peptide

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological reseach on expression of inflammatory mediators in murine models of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by T2 antigen

et al. 2019

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“…T2 peptide was dissolved to 1 mg/ml of reserves for a combination of an equal concentration of CFA before immunization. [23] Except for the control group, the other groups were all given a multipoint subcutaneous injection on days 0 and 14. Every time, mice were injected with 200 ll of a mixture subcutaneously with five injections separately in the back.…”

Section: Immunizationmentioning

confidence: 99%

Therapeutic effects of Qianlie Tongli decoction on chronic prostatitis/chronic pelvic pain syndrome induced by peptide T2 in mice

Cui

Naveed

Wang

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives This study was undertaken to reveal therapeutic effects and the preliminary mechanism of Chinese medicine formula Qianlie Tongli decoction (QTD) in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods A total of 50 male C57BL/6 mice were randomly divided into five groups. All groups except the control group were injected subcutaneously T2 peptide emulsion, which induced the CP/CPPS model. After the induction of CP/CPPS, the model group was given normal saline by oral gavage while low‐dose, medium‐dose and high‐dose groups were treated with Chinese medicine formula. Micturition habits and pain behaviour of mice were analysed for each group. Haematoxylin and eosin (H&E) staining was used to investigate prostate inflammation. The serum level of tumour necrosis factor‐α (TNF‐α) was measured by enzyme‐linked immunosorbent assay (ELISA) kit. Key findings Chinese medicine formula significantly reduced the number of urine spots and improved pain response frequency in the medium‐dose and high‐dose group. The high‐dose group showed reduced considerably inflammatory lesion and inflammatory cell infiltration than the low‐dose and medium‐dose groups. Serum levels of TNF‐α in the high‐dose group were significantly reduced compared with the model group. Conclusions The results demonstrated the therapeutic effects of Qianlie Tongli decoction in CP/CPPS mice by analysing clinically relevant symptoms (urinary tract system, pelvic pain and prostate inflammation) and preliminarily explored the inflammatory‐related treatment mechanisms by measuring TNF‐α.

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“…T2 peptide was dissolved to 1mg/ml of reserves for a combination of an equal concentration of CFA before immunization [23]. Except for the control group, the other groups were all given a multipoint subcutaneous injection on days 0 and 14.…”

Section: Immunizationmentioning

confidence: 99%

Therapeutic Effects of Traditional Chinese Medicine Formula Qianlie Tongli Decoction on Chronic Prostatitis/Chronic Pelvic Pain Syndrome Induced by Peptide T2 in Mice

Cui¹,

Naveed²,

Wang³

et al. 2020

Preprint

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Objectives: This study was undertaken to reveal therapeutic effects and the preliminary mechanism of Chinese medicine formula Qianlie Tongli decoction (QTD) in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods: A total of 50 male C57BL/6 mice were randomly divided into five groups. All groups except the control group were injected subcutaneously T2 peptide emulsion, which induced the CP/CPPS model. After the induction of CP/CPPS, the model group was given 0.9% NaCl by oral gavage while low-dose, medium-dose, and high-dose groups were treated with Chinese medicine formula. Micturition habits and pain behavior of mice were analyzed for each group. Hematoxylin and eosin staining were used to investigate prostate inflammation. The serum level of tumor necrosis factor-α (TNF-α) was measured by enzyme-linked immunosorbent assay (ELISA) kit. Key findings: Chinese medicine formula significantly reduced the number of urine spots and improved pain response frequency in the medium-dose and high-dose group. The high-dose group showed reduced considerably inflammatory lesion and inflammatory cell infiltration than the low-dose and medium-dose groups. Serum levels of TNF-α in the high-dose group were significantly reduced compared with the model group. Conclusions: The results demonstrated the therapeutic effects of Qianlie Tongli decoction in CP/CPPS mice by analyzing clinically relevant symptoms (urinary tract system, pelvic pain, and prostate inflammation), and preliminary explored the inflammatory-related treatment mechanisms by measuring TNF-α.

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Experimental rodent models of chronic prostatitis and evaluation criteria

Cited by 32 publications

References 41 publications

Morphological reseach on expression of inflammatory mediators in murine models of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by T2 antigen

Morphological reseach on expression of inflammatory mediators in murine models of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by T2 antigen

Therapeutic effects of Qianlie Tongli decoction on chronic prostatitis/chronic pelvic pain syndrome induced by peptide T2 in mice

Therapeutic Effects of Traditional Chinese Medicine Formula Qianlie Tongli Decoction on Chronic Prostatitis/Chronic Pelvic Pain Syndrome Induced by Peptide T2 in Mice

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