Experimental sensitization of guinea-pigs to nickel and patch testing with metal samples

Zissu, D.; Cavelier, C.; Céaurriz, J. de

doi:10.1016/0278-6915(87)90309-7

Cited by 19 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reproducible results from challenge to rechallenge with different sensitizers in the GPMT have been reported by several authors (1, 5,12,19,20). Others have observed a reduced sensitization frequency from challenge to rechallenge (19,21).…”

Section: Reproducibility Of Patch Test Reactions From Challenge To Rementioning

confidence: 69%

“…It has been demonstrated that FCAtreated guinea pigs have a lower skin irritation threshold and that guinea pigs stressed by concomitant allergic or irritant inflammation remote from the site of skin testing can have a generalized state of hyperreactivity, also known as the "angry back" or excited skin syndrome (5)(6)(7)(8). An altered state of reactivity in the skin may sometimes lead to questionable challenge results in test animals and, occasionally, non-specific irritant responses in control animals.…”

Section: Irritancy and "False-positive" Reactionsmentioning

confidence: 99%

See 1 more Smart Citation

The value and limitations of rechallenge in the guinea pig maximization test

1996

View full text Add to dashboard Cite

The guinea pig maximization test (GPMT) has played a primary role in the evaluation of potential skin contact sensitizers for 25 years. In the OECD Guideline 406 from 1993, it is specifically suggested that equivocal results from the initial challenge in the GPMT should be evaluated further with a repeated challenge. However, there exist few published rechallenge data and the guideline does not describe how rechallenge data should be interpreted. In this paper, we have used examples from published results to illustrate both the positive value and the limitations of repeated challenges, including cross challenge. Testing with modified concentrations may also help to indicate whether or not the response is allergic in nature, particularly where there has been a low level of skin reaction observed in shamtreated controls, or where a low level of skin reaction is the dominant response in the test animals. In conclusion, the data presented demonstrate that, as a tool for the investigation of skin sensitizing potential, the GPMT can benefit from an experienced scientific evaluation of rechallenge data, but that this information should not be treated in a mechanistic fashion.

show abstract

Section: Reproducibility Of Patch Test Reactions From Challenge To Rementioning

confidence: 69%

Section: Irritancy and "False-positive" Reactionsmentioning

confidence: 99%

The value and limitations of rechallenge in the guinea pig maximization test

1996

View full text Add to dashboard Cite

show abstract

“…Despite many efforts spent in developing an experi mental animal model for nickel CS, only few reports on successful nickel sensitization have appeared [25][26][27][28], The sensitization procedures described, however, are gener ally time-consuming or difficult to reproduce and often re sult in only low responder rates [reviewed in ref . 12].…”

Section: Discussionmentioning

confidence: 99%

Development of a Concomitant Nickel and Chromium Sensitization Model in the Guinea Pig

J²,

Boden³

et al. 1992

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Although nickel allergy is the most frequent contact hypersensitivity in man, reports on successful nickel sensitization in experimental animals are scarce. Chromium hypersensitivity, on the other hand, is readily induced in guinea pigs. In this study we set out to obtain reproducible nickel sensitization in guinea pigs, in order to establish an animal model for immunospecific tolerance and desensitization studies in which two non-cross-reacting metal allergens, chromium and nickel, could be studied simultaneously. Strong and reproducible sensitization to nickel was achieved by injecting low amounts of Freund’s complete adjuvant and nickel sulfate in a split-adjuvant procedure. Strong erythematous reactions were observed as early as 14 days after sensitization and could be elicited both by intradermal and open epicutaneous challenges. Optimal evaluation was with nickel sulfate administered epicutaneously in 40% dimethyl sulfoxide to enhance skin penetration. Hypersensitivity could be transferred with lymphocytes and not with serum. Sensitization procedures for nickel and chromium then could successfully be combined in a double sensitization procedure. With four different guinea pig strains no genetic restriction was observed for the induction of nickel or chromium sensitivity. However, for both metals a clear sex and age dependence was observed: female guinea pigs reached a higher degree of sensitization than males, whereas sensitization in young animals was relatively weak.

show abstract

“…The expenmental protocol has already been described m a previOus publicatiOn (2). Bnefly, gumea pigs were pamted 5 days/week for 4 weeks w1th 1% mckel sulphate m lanolin.…”

Section: Experzmental Sensztization Of Gumea Pzgs To Mckel and Patch mentioning

confidence: 99%

Nickel allergy: tolerance to metallic surface‐plated samples in nickel‐sensitive humans and guinea pigs

Cavelier

Foussereau

Gille³

et al. 1988

Contact Dermatitis

Self Cite

View full text Add to dashboard Cite

The purpose of this work is to evaluate in nickel-sensitive patients and guinea pigs the tolerance to nickel samples, surface-plated with one or several metals of varying structures and thicknesses. All the metal samples elicited allergic reactions in the guinea pig. In humans, absolute tolerance was not observed for any sample. In humans, the interposing of a layer of bright copper between nickel and surface chrome greatly increased the tolerance.

show abstract

Experimental sensitization of guinea-pigs to nickel and patch testing with metal samples

Cited by 19 publications

References 13 publications

The value and limitations of rechallenge in the guinea pig maximization test

The value and limitations of rechallenge in the guinea pig maximization test

Development of a Concomitant Nickel and Chromium Sensitization Model in the Guinea Pig

Nickel allergy: tolerance to metallic surface‐plated samples in nickel‐sensitive humans and guinea pigs

Contact Info

Product

Resources

About