Experimental setup for studying the effects of alpha particles on zebrafish embryos

Yum, E.H.W.; Ng, Colin; Lin, Aiping; Cheng, Shuk Han; Yu, K.N.

doi:10.1016/j.nimb.2007.07.024

Cited by 30 publications

(28 citation statements)

References 18 publications

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“…Among the 54 irradiated embryos, five abnormal developments were identified while no abnormal case was found in all the 52 control embryos. As such, Yum et al [14] demonstrated the feasibility to inflict observable radiation effects of alpha particles on dechorionated zebrafish embryos through irradiation for 240 s. We therefore Fig. 3.…”

Section: Magnitudes Of Priming and Challenging Dosesmentioning

confidence: 82%

“…Yum et al [14] devised an experimental setup to study effects of alpha particles on zebrafish embryos. In the outset, they highlighted challenges in avoiding excessive or variable absorption of the energy of alpha particles before they could actually reach the cells of the embryos.…”

Section: Setup For Alpha-particle Irradiationmentioning

confidence: 99%

“…As such, Yum et al [14] studied the feasibility to use PADC films as the support substrates for the dechorionated zebrafish embryos for alpha-particle irradiation. PADC films are biocompatible [15] and the thickness can be controlled relatively accurately and conveniently through chemical etching [16].…”

Section: Setup For Alpha-particle Irradiationmentioning

confidence: 99%

“…Yum et al [14] irradiated dechorionated zebrafish embryos at 4 hpf with alpha particles from a planar 241 Am source (with an alpha-particle energy of 5.49 MeV under vacuum and an activity of 0.1151 lCi) for 240 s from the side of the PADC film. Among the 54 irradiated embryos, five abnormal developments were identified while no abnormal case was found in all the 52 control embryos.…”

Section: Magnitudes Of Priming and Challenging Dosesmentioning

confidence: 99%

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Designing experimental setup and procedures for studying alpha-particle-induced adaptive response in zebrafish embryos in vivo

Choi

Lam

Chong

et al. 2010

Nuclear Instruments and Methods in Physics Research Section B:

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Section: Magnitudes Of Priming and Challenging Dosesmentioning

confidence: 82%

Section: Setup For Alpha-particle Irradiationmentioning

confidence: 99%

Section: Setup For Alpha-particle Irradiationmentioning

confidence: 99%

Section: Magnitudes Of Priming and Challenging Dosesmentioning

confidence: 99%

See 2 more Smart Citations

Designing experimental setup and procedures for studying alpha-particle-induced adaptive response in zebrafish embryos in vivo

Choi

Lam

Chong

et al. 2010

Nuclear Instruments and Methods in Physics Research Section B:

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“…Studies on non-targeted effects of high-LET ionizing radiation using zebrafish embryos as the in vivo model began with studies on the effects of alpha particles, together with the design of a special experimental setup and procedures [213][214][215]. The biggest challenge in these experiments was posed by the short ranges of alpha particles.…”

Section: Pioneer Work On Studies Of the Effect Of High-let Ionizing mentioning

confidence: 99%

Embryos of the zebrafish Danio rerio in studies of non-targeted effects of ionizing radiation

Choi

2015

Cancer Letters

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Alpha radiation exposure decreases apoptotic cells in zebrafish embryos subsequently exposed to the chemical stressor, Cd

Tung

Choi

et al. 2012

Environ Sci Pollut Res

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The aim of this study was to demonstrate that zebrafish embryos subjected to a priming exposure provided by one environmental stressor (low-dose alpha particles) can induce an adaptive response against a subsequent challenging exposure provided by another environmental stressor (heavy metal Cd). The effect thus identified would be an antagonistic multiple stressor effect. The effects of alpha particle radiation and/or Cd on whole embryos were studied through quantification of apoptotic signals at 24 h post-fertilization (hpf). Embryos were stained with the vital dye acridine orange, followed by counting the stained cells. For each set of experiments, 30 dechorionated embryos were divided into three groups, each having ten embryos. The three groups of embryos were referred to as (A) the control group, which received no more further treatments after dechorionation, (B) Cd-treated group, which did not receive any priming exposure and would receive a challenging exposure at 10 hpf and (C) (alpha + Cd)-treated group, which would receive both priming and challenging exposures. We defined the normalized net number of apoptotic signals in the (alpha + Cd)-treated group as N (C) * = [(apoptotic signals for (alpha + Cd)-treated group - average apoptotic signals for the corresponding control group)/average apoptotic signals for the corresponding control group] and that in the Cd-treated group as N (B)* = [(apoptotic signals for Cd-treated group - average apoptotic signals for the corresponding control group)/ average apoptotic signals for the corresponding control group]. By using the non-parametric Mann-Whitney U statistic, we were able to show that N (C) * was significantly smaller than N (B) *(p = 0.006). These demonstrated an antagonistic multiple stressor effect between ionizing radiation and Cd through the induction of an adaptive response by the ionizing radiation against subsequent exposures to Cd.

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Experimental setup for studying the effects of alpha particles on zebrafish embryos

Cited by 30 publications

References 18 publications

Designing experimental setup and procedures for studying alpha-particle-induced adaptive response in zebrafish embryos in vivo

Designing experimental setup and procedures for studying alpha-particle-induced adaptive response in zebrafish embryos in vivo

Embryos of the zebrafish Danio rerio in studies of non-targeted effects of ionizing radiation

Alpha radiation exposure decreases apoptotic cells in zebrafish embryos subsequently exposed to the chemical stressor, Cd

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