Experimental Studies of Intestinal Ion and Water Transport

Holtug, Klavs; Hansen, Mark Berner; Skadhauge, Erik

doi:10.3109/00365529609094565

Cited by 45 publications

(30 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In vivo, water follows the osmotic gradient created by actively transported ions [15]. Measuring Isc in Ussing chambers can therefore give indications on possible water movement in vivo and be useful to understand the pathophysiology of diarrhea [16,21]. This can be done at a basal level, without stimulation of the tissue, or after the addition in the chambers of pharmacological or natural substances inducing an active transport of ions (Fig.…”

Section: Type Of Experiments Performed With Ussing Chambersmentioning

confidence: 99%

“…The ability to maintain intestinal tissue alive in a controlled and an as-close-as-possible-to-physiological environment for a couple of hours has brought scientists to use this technique to study the permeability of intestinal tissue to various molecules, including ions [21], nutrients [1], drugs [25], small molecule probes [20] and macromolecules [23]. This can be done by simply adding the labeled compound in one side of the chamber and sampling the solution in the other side to determine the permeability of the tissue to this given compound.…”

Section: Type Of Experiments Performed With Ussing Chambersmentioning

confidence: 99%

See 1 more Smart Citation

The Ussing chamber technique to evaluate alternatives to in-feed antibiotics for young pigs

Boudry

2005

Anim. Res.

View full text Add to dashboard Cite

-The recent ban of in-feed antibiotics in animal production has brought scientists to find alternatives to in-feed antibiotics. The Ussing chamber is an in vitro technique in which intestinal tissue is collected and immediately mounted as a flat sheet between two half-chambers, establishing a luminal and a serosal side. This technique allows the measurement of actively transported ions as well as the permeability of the tissues, two parameters relevant for the evaluation of gut health. Ussing chambers have been used to describe the changes of intestinal physiology occurring at weaning. We observed that weaning induces an acute alteration of the epithelial barrier function and electrolyte transport in the first days. Long-lasting modifications (15 days after weaning) have also been observed. Alternatives to in-feed antibiotics can be evaluated by studying the intestinal physiology of animals treated with those alternatives. A benefic effect of pro-and pre-biotics on the rat intestine, human biopsies or epithelial cell monolayers has been demonstrated using Ussing chambers. In pigs, the influence of the source of dietary ingredients or the physical form of the diet seems limited. A few results obtained in pigs with pre-or probiotics appear less convincing than in laboratory animals. Another way to use Ussing chambers is to incubate the intestinal tissue with different substances added directly into the chambers. Some substances can be detrimental to the intestine, inducing electrolyte secretion or decreasing barrier function. However, some substances show a beneficial effect. Although this approach has limits and should be combined with in vivo measurements, it constitutes a rapid way to evaluate the effect of substances on intestinal physiology and can also, at least partly, elucidate the mechanisms of action of those alternatives. perturbations intestinales au moment du sevrage. Nous avons ainsi pu montrer que le sevrage induit des altérations transitoires de la physiologie intestinale ainsi que des modifications à plus long terme. Les alternatives aux antibiotiques peuvent être évaluées par l'étude de la physiologie intestinale des animaux traités avec ces alternatives. Un effet bénéfique des pro-et prébiotiques sur l'intestin de rat, des biopsies intestinales humaines ou des monocouches de cellules épithéliales intestinales a été démontré en chambres d'Ussing. Chez le Porc, l'influence des sources de matières premières ou de la forme physique de l'aliment semble limitée. De plus, les rares résultats obtenus chez le porc quant à l'effet des pré-et probiotiques semblent moins convaincants que chez les animaux de laboratoire. Une autre façon d'utiliser les chambres d'Ussing est d'incuber l'intestin avec différentes substances ajoutées directement dans les chambres. Certains composés semblent avoir un effet délétère sur l'intestin, induisant des sécrétions d'électrolytes ou des modifications de la barrière épithéliale. Cependant, certaines substances ont montré un effet bénéfique. Bien que cette technique...

show abstract

Section: Type Of Experiments Performed With Ussing Chambersmentioning

confidence: 99%

Section: Type Of Experiments Performed With Ussing Chambersmentioning

confidence: 99%

The Ussing chamber technique to evaluate alternatives to in-feed antibiotics for young pigs

Boudry

2005

Anim. Res.

View full text Add to dashboard Cite

show abstract

“…It is widely accepted that increased luminal chloride concentrations can be due to decreased absorption in the villus cells and/or increased secretion in the crypt cells (5,17,18,23). To date, however, there has been little information concerning a possible dysfunction in the chloride absorptive process in rotavirus disease.…”

mentioning

confidence: 97%

Rotavirus Infection Stimulates the Cl⁻Reabsorption Process across the Intestinal Brush-Border Membrane of Young Rabbits

Lorrot

Martin

Vasseur

2003

J Virol

View full text Add to dashboard Cite

Rotavirus is a major cause of infantile gastroenteritis worldwide. However, the mechanisms underlying fluid and electrolyte secretion associated with diarrhea remain largely unknown. We investigated the hypothesis that loss of Cl ؊ into the luminal contents during rotavirus infection may be caused by a dysfunction in the chloride absorptive capacity across the intestinal brush-border membrane (BBM). The luminal Cl ؊ concentrations in the entire small intestine of young rabbits infected with lapine rotavirus decreased at 1 and 2 days postinfection (dpi), indicating net Cl ؊ absorption. At 7 dpi, luminal Cl ؊ concentrations were slightly increased, indicating a moderate net Cl ؊ secretion. By using a rapid filtration technique, 36 Cl uptake across BBM was quantified by modulating the alkali-metal ion, electrical, chloride, and/or proton gradients. Rotavirus infection caused an identical, 127% ؎ 24% increase in all Cl ؊ uptake activities (Cl ؊ /H ؉ symport, Cl ؊ conductance, and Cl ؊ /anion exchange) observed across the intestinal BBM. The rotavirus activating effects on the symporter started at 1 dpi and persisted up to 7 dpi. Kinetic analyses revealed that rotavirus selectively affected the capacity parameter characterizing the symporter. We report the novel observation that rotavirus infection stimulated the Cl ؊ reabsorption process across the intestinal BBM. We propose that the massive Cl ؊ reabsorption in villi could partly overwhelm chloride secretion in crypt cells, which possibly increases during rotavirus diarrhea, the resulting imbalance leading to a moderate net chloride secretion.

show abstract

“…Water permeabilities across these membranes are 10-to 100-fold greater than that observed in phospholipid bilayers. The ratio of osmotic permeabilities (P f , determined by the measurement of bulk water flow in response to an osmotic gradient) to diffusive permeabilities (P d , measurement of the flux of isotopic water under conditions of equal osmolality) are far greater than 1 (ratio observed in lipid bilayers) (196)(197)(198)(199)(200)(201)(202)(203)(204)(205)(206)(207)(208)(209)(210)(211)(212)(213). This increased ratio is characteristic of water channel-mediated fluid transport.…”

Section: Water Transport and Blastocyst Formationmentioning

confidence: 99%

Regulation of blastocyst formation

Watson¹

2001

Front Biosci

115

View full text Add to dashboard Cite

Experimental Studies of Intestinal Ion and Water Transport

Cited by 45 publications

References 40 publications

The Ussing chamber technique to evaluate alternatives to in-feed antibiotics for young pigs

The Ussing chamber technique to evaluate alternatives to in-feed antibiotics for young pigs

Rotavirus Infection Stimulates the Cl⁻Reabsorption Process across the Intestinal Brush-Border Membrane of Young Rabbits

Regulation of blastocyst formation

Contact Info

Product

Resources

About

Experimental Studies of Intestinal Ion and Water Transport

Cited by 45 publications

References 40 publications

The Ussing chamber technique to evaluate alternatives to in-feed antibiotics for young pigs

The Ussing chamber technique to evaluate alternatives to in-feed antibiotics for young pigs

Rotavirus Infection Stimulates the Cl−Reabsorption Process across the Intestinal Brush-Border Membrane of Young Rabbits

Regulation of blastocyst formation

Contact Info

Product

Resources

About

Rotavirus Infection Stimulates the Cl⁻Reabsorption Process across the Intestinal Brush-Border Membrane of Young Rabbits