Experimental studies on the survival of pathogenic and indicator bacteria in aerated and non-aerated cattle and pig slurry

Munch, Bente; Larsen, Holger Errebo; Aalbæck, Bent

doi:10.1016/0269-7483(87)90099-1

Cited by 40 publications

(29 citation statements)

References 8 publications

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“…The only significant decrease occurred between the storage tank and the pond. This stability of fecal indicator concentrations observed during pig slurry anaerobic storage contrasts with the decrease previously reported in batch studies (26,27,33). The difference may have been due to the constant addition of fresh feces to the slurry in real systems, which may logically lead to stabilization of the number of fecal indicators.…”

Section: Discussioncontrasting

confidence: 85%

“…According to this hypothesis, important microbial changes were observed in this study when the community was moved from the feces to the manure and then from the storage tank to the pond. Previous studies have demonstrated the stronger effects of drastic conditions like aeration or thermophilic treatment for modifying the pig slurry microbial community (17,20,26,27).…”

Section: Discussionmentioning

confidence: 99%

“…Although this type of husbandry is a success for pig production, the different stages of waste management generate severe air, soil, and water pollution (5,14,24,25,46,47). Moreover, spreading large amounts of manure onto fields raises questions about the sanitary aspects of such practices (5,7,26,27,30).…”

mentioning

confidence: 99%

“…Despite the increasing interest now being paid to characterization of pig slurry microbiology (48), few workers have tried to evaluate the impact of pig slurry storage conditions on the composition of the slurry microbial community (2,26,27,33,49). In most of the studies the researchers used cultural methods and focused on the fate of pathogenic or fecal indicators (26,27).…”

mentioning

confidence: 99%

“…In most of the studies the researchers used cultural methods and focused on the fate of pathogenic or fecal indicators (26,27). These studies have shown that the numbers of fecal indicators and pathogenic bacteria decrease rapidly and exponentially with time during pig slurry storage.…”

mentioning

confidence: 99%

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Dynamics of a Pig Slurry Microbial Community during Anaerobic Storage and Management

Peu¹,

Brugère

Pourcher

et al. 2006

Appl Environ Microbiol

108

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The microbial community of a pig slurry on a farm was monitored for 6 months using both molecular and cultural approaches. Sampling was carried out at all the different stages of effluent handling, from the rearing build-up to slurry spreading. Total DNA of each sample was extracted and analyzed by PCR-single-strand conformation polymorphism (SSCP) analysis using primers targeting the 16S rRNA genes from the archaeal and bacterial domains and also the Eubacterium-Clostridium, Bacillus-Streptococcus-Lactobacillus, and Bacteroides-Prevotella groups. A comparison of the SSCP profiles showed that there were rapid changes in the dominant bacterial community during the first 2 weeks of anaerobic storage and that the community was relatively stable thereafter. Several bacterial populations, identified as populations closely related to uncultured Clostridium and Porphyromonas and to Lactobacillus and Streptococcus cultured species commonly isolated from pig feces, remained present and dominant from the rearing build-up to the time of spreading. Enumeration of fecal indicators (enterococci and Escherichia coli) performed in parallel using cultural methods revealed the same trends. On the other hand, the archaeal community adapted slowly during pig slurry storage, and its diversity increased. A shift between two hydrogenotrophic methanogenic Methanobrevibacter populations from the storage pit to the pond was observed. Microorganisms present in pig slurry at the time of spreading could not be detected in soil after spreading by either molecular or cultural techniques, probably because of the detection limit inherent in the two techniques.

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Section: Discussioncontrasting

confidence: 85%

Section: Discussionmentioning

confidence: 99%