Mechanical belt sanding is critical in the manufacturing of bamboo and bamboo products, where surface roughness is commonly used to quantitatively evaluate the surface quality. In this study, flattened bamboo workpieces were sanded using P80 and P120 abrasive belts to create different surfaces. The linear roughness parameters, namely Rz, Ra, Rq, Rsk, Rku, and Rmr(c), were measured using both a stylus profilometer and a 3D profilometer. Statistical t-tests were conducted to determine the significance of differences between the two methods. Additionally, roughness profiles were analyzed in the frequency domain using Fast Fourier Transform (FFT) and Power Spectral Density (PSD) methods. A Random Forest (RF) regression model was also developed to predict the roughness values and figure out the dominant factors between granularity and measurement methods. The results revealed that both the stylus and 3D profilometers provided reliable comparisons of Rz, Ra, Rq, and Rmr (50%) for different grit sizes. However, resolution differences between the two methods were found to be critical for accurately interpreting roughness values. Variations in Rsk and Rku highlighted differences in sensitivity and detection range, particularly at finer scales, between the two methods. The stylus profilometer, with its higher spatial resolution and finer sampling density, demonstrated greater sensitivity to finer surface details. This was consistent with the FFT and PSD analyses, which showed that the stylus profilometer captured higher-frequency surface components more effectively. Furthermore, the RF model indicated that the choice of measurement method had negligible impact on the evaluation of the selected roughness parameters, suggesting that standardizing measurement techniques may not be essential for consistent roughness assessments of sanded bamboo surfaces.