Biodiesel is a renewable energy source which is gaining prominence as an alternative fuel over fossil diesel for different applications. Due to their higher viscosity and lower volatility, biodiesels are blended with diesel in various proportions. B20 blends are viable and sustainable solutions in diesel engines with acceptable engine performance as they can replace 20% fossil fuel usage. Biodiesel blends are slightly viscous as compared with diesel and can be used in common rail direct injection (CRDI) engines which provide high pressure injection using an electronic control unit (ECU) with fuel flexibility. In view of this, B20 blends of three biodiesels derived from cashew nutshell (CHNOB (B20)), jackfruit seed (JACKSOB (B20)), and Jamun seed (JAMNSOB (B20)) oils are used in a modified single-cylinder high-pressure-assisted CRDI diesel engine. At a BP of 5.2 kW, for JAMNSOB (B20) operation, BTE, NOx, and PP increased 4.04%, 0.56%, and 5.4%, respectively, and smoke, HC, CO, ID, and CD decreased 5.12%, 6.25%, 2.75%, 5.15%, and 6.25%, respectively, as compared with jackfruit B20 operation.