The effect of fuel depth on flame spread over aviation kerosene and the characteristics of the subsurface flow has been investigated using schlieren system and a high-speed camera. For flame spread over aviation kerosene, there are shallow pool and deep pool regimes separated by a fuel depth of 8 mm. For the shallow pool regime, the pulsation amplitude of the main flame and the precursor flame increases, but the pulsation frequency of the flames decreases with an increase in fuel depth. For the deep pool regime, fuel depth has no obvious effect on the flame pulsation and the ratio of the length to the depth of the subsurface flow. The theoretical model of the rate of the subsurface flow based on the surface tension effect agrees well with experimental values.