Experimental Study of Interstitial Injection of Trypsin and Trypsinogen into the Pancreas

Inhibitory effect of various cytostatics and cycloheximide on acute experimental pancreatitis in rats.

“…Several methods of producing acute experimental pancreatitis are known-for instance, perfusing the bile ducts (Elliott et al, 1958;Beck et al, 1964), interstitially administering different compounds (Keith et al, 1958;Waterman et al, 1969), or by the action of various stimuli or inducers (Bawnik et al, 1974;Schiller et al, 1974;Backwinkel et al, 1975;Lombardi et al, 1975). In the present study 90 male rats (Wistar, 250-300 g) were kept on a standard diet and separated into several groups.…”

“…In the present study 90 male rats (Wistar, 250-300 g) were kept on a standard diet and separated into several groups. Laparotomy was carried out under mild ether narcosis and 0-6 ml 5 % bile solution was administered interstitially into the 913 pancreas (Keith et al, 1958). After six hours of treatment the animals were killed by cervical dislocation and the volume of abdominal fluid was measured.…”

A fresh approach to the pathogenesis of pancreatitis.

“…Rich and Duff confirmed this work and described specific vascular lesions which they found in all their cases of pancreatitis whether human or experimental; commercial trypsin injected subcutaneously in dogs produced the same vascular lesions. Keith, Barnes, and Denkewalter (1958) showed that acute pancreatitis could be produced regularly in dogs by injecting trypsin into the pancreatic interstitial tissue, but trypsinogen did not have the same effect. In contrast, some workers have doubted the importance of activation of enzymes in pancreatitis on the basis of experiments in which bile is injected forcibly into the pancreatic duct (Jenson, Imamoglu, Root, and Wangensteen, 1961;Beck, Pinter, Solymar, McKenna, and Ritchie, 1962); as bile does not activate trypsinogen these experiments could not be expected to prove anything.…”