Polyurethane cement (PUC) is a kind of high-strength composite bending and strengthening material that is made by adding an appropriate amount of super fine aggregate, such as cement, into the polyurethane matrix. Carbon nanofiber polyurethane cement (CNFPUC) prepared by adding an appropriate amount of carbon nanofiber (CNF) can significantly reduce the resistivity of PUC. In order to reveal the change regulation of electrical resistivity of CNFPUC under fatigue load, the four-point bending fatigue life test of CNFPUC under different temperatures and stress levels was conducted by using a multifunction test device on a mixture of UTM-30 asphalt. The results showed that the resistivity increased slightly with the increase of bending strain in the process of the CNFPUC bending test, which presented a two-stage trend of a small increase and a sharp increase, and the maximum bending tensile stress was up to 26.65 MPa. Under periodic load, the resistivity increased with the increase of stress level and cycle times. When the damage amount reached 90%, the fatigue resistivity increased sharply, and with the increase of stress level, the effect of temperature on resistivity decreased gradually. This study may provide a theoretical basis for practical engineering applications.