In mines where the natural caving method is used, the frequent occurrence of underground debris flows and the complex mine environments make it difficult to prevent and control underground debris flows. The source is one of the critical conditions for the formation of debris flows, and studying the impact of source material gradation on underground debris-flow disasters can effectively help prevent and control these occurrences. This paper describes a multiscale study of underground debris flows using physical model experiments and the discrete-element method (PFC3D) to understand the impact of the source material gradation on the disaster mechanism of underground debris flows from macroscopic and microscopic perspectives. Macroscopically, an increase in content of medium and large particles in the gradation will enhance the instantaneous destructive force. Large particles can more easily cause disasters than medium and fine particles with the same content, but the disaster-causing ability is minimized when the contents of medium and large particles exceed 50% and 60%, respectively. With increasing fine particle content, the long-distance disaster-causing ability and duration is increased. On the microscopic level, the source-level pairs affect the initial flow mode, concentration area of the force chain, average velocity, average runout distance, and change in energy of the underground debris flow. Among them, the proportion of large particles in the gradation significantly affects the change in kinetic energy, change in dissipative energy, time to reach the peak kinetic energy, and time of coincidence of dissipative energy and gravitational potential energy. The process of underground debris flow can be divided into a “sudden stage”, a “continuous impact stage”, and a “convergence and accumulation stage”. This work reveals the close relationship between source material gradation and the disaster mechanism of underground debris flows and highlights the necessity of considering the source material gradation in the prevention and control of underground debris flows. It can provide an important basic theory for the study of environmental and urban sustainable development.