Experimental study of the susceptibility of a European <i>Aedes albopictus</i> strain to dengue virus under a simulated Mediterranean temperature regime

Brustolin, Marco; Santamaría, C. Berástegi; Napp, Sebastián; Verdún, Marta; Rivas, Raquel; Pujol, Núria; Talavera, Sandra; Busquets, Núria

doi:10.1111/mve.12325

Cited by 11 publications

(8 citation statements)

References 28 publications

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“…In fact, a recent study has experimentally shown that an Ae. albopictus strain from Catalonia could sustain DENV-2 replication under a simulated Mediterranean temperature regime [38], which would be in agreement with our detection of a high viral load in the DENV-positive mosquito pool in the summer 2015.…”

Section: Discussionsupporting

confidence: 89%

Arbovirus surveillance: first dengue virus detection in local Aedes albopictus mosquitoes in Europe, Catalonia, Spain, 2015

et al. 2018

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Dengue has emerged as the most important viral mosquito-borne disease globally. The current risk of dengue outbreaks in Europe appeared with the introduction of the vector Aedes albopictus mosquito in Mediterranean countries. Considering the increasing frequency of dengue epidemics worldwide and the movement of viraemic hosts, it is expected that new autochthonous cases will occur in the future in Europe. Arbovirus surveillance started in Catalonia in 2015 to monitor imported cases and detect possible local arboviral transmission. During 2015, 131 patients with a recent travel history to endemic countries were tested for dengue virus (DENV) and 65 dengue cases were detected. Twenty-eight patients with a febrile illness were viraemic, as demonstrated by a positive real-time RT-PCR test for DENV in serum samples. Entomological investigations around the viraemic cases led to the detection of DENV in a pool of local Ae. albopictus captured in the residency of one case. The sequence of the DENV envelope gene detected in the mosquito pool was identical to that detected in the patient. Our results show how entomological surveillance conducted around viraemic travellers can be effective for early detection of DENV in mosquitoes and thus might help to prevent possible autochthonous transmission.

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Section: Discussionsupporting

confidence: 89%

Arbovirus surveillance: first dengue virus detection in local Aedes albopictus mosquitoes in Europe, Catalonia, Spain, 2015

et al. 2018

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“…albopictus and Ae. aegypti (a recognized epidemic vector) showed similar TE rates for CHIKV [126,[147][148][149][150][151][152], DENV-1 [153][154][155][156][157], and DENV-2 [126,147,154,155,[157][158][159], but Ae. aegypti was more efficient at transmitting ZIKV [126,[160][161][162][163][164][165][166][167][168][169][170][171] and YFV [127,128,[172][173][174][175].…”

Section: Arbovirus Transmissionmentioning

confidence: 99%

A Systematic Review: Is Aedes albopictus an Efficient Bridge Vector for Zoonotic Arboviruses?

et al. 2020

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Mosquito-borne arboviruses are increasing due to human disturbances of natural ecosystems and globalization of trade and travel. These anthropic changes may affect mosquito communities by modulating ecological traits that influence the “spill-over” dynamics of zoonotic pathogens, especially at the interface between natural and human environments. Particularly, the global invasion of Aedes albopictus is observed not only across urban and peri-urban settings, but also in newly invaded areas in natural settings. This could foster the interaction of Ae. albopictus with wildlife, including local reservoirs of enzootic arboviruses, with implications for the potential zoonotic transfer of pathogens. To evaluate the potential of Ae. albopictus as a bridge vector of arboviruses between wildlife and humans, we performed a bibliographic search and analysis focusing on three components: (1) The capacity of Ae. albopictus to exploit natural larval breeding sites, (2) the blood-feeding behaviour of Ae. albopictus, and (3) Ae. albopictus’ vector competence for arboviruses. Our analysis confirms the potential of Ae. albopictus as a bridge vector based on its colonization of natural breeding sites in newly invaded areas, its opportunistic feeding behaviour together with the preference for human blood, and the competence to transmit 14 arboviruses.

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“…The best way to estimate the risk of dengue fever outbreak is to measure whether mosquitoes are infected with dengue virus while simulating environmental conditions at mosquito collection sites [68]. In this study, we used a temperature and humidity recorder to record the hourly temperature and humidity of mosquitoes living in the field, and simulated the hourly temperature and humidity changes in the field environment in an artificial climate box, which was more detailed than the temperature fluctuation conditions set by Brustolin et al (daytime temperature 26 °C, nighttime temperature 22 °C [68]. In the present study, the temperature fluctuated from, the relative humidity fluctuated from 55.4 to 100%, and the average humidity was 84% in summer.…”

Section: Discussionmentioning

confidence: 99%

Effects of Guangzhou seasonal climate change on the development of Aedes albopictus and its susceptibility to DENV-2

Zheng

et al. 2021

Preprint

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The susceptibility of Asian tiger mosquitoes to DENV-2 in different seasons was observed in simulated field environments as a reference to design dengue fever control strategies in Guangzhou. The life table experiments of mosquitoes in four seasons were carried out in the field. The susceptibility of Ae. albopictus to dengue virus was observed in both environments in Guangzhou in summer and winter. Ae. albopictus was infected with dengue virus by oral feeding. On day 7 and 14 after infection, the viral load in the head, ovary, and midgut of the mosquito was detected using real-time fluorescent quantitative PCR. Immune-associated gene expression in infected mosquitoes was performed using quantitative real-time reverse transcriptase PCR. The hatching rate and pupation rate of Ae. albopictus larvae in different seasons differed significantly. The winter hatching rate of larvae was lower than that in summer, and the incubation time was longer than in summer. In the winter field environment, Ae. albopictus still underwent basic growth and development processes. Mosquitoes in the simulated field environment were more susceptible to DENV-2 than those in the simulated laboratory environment. In the midgut, viral RNA levels on day 7 in summer were higher than those on day 7 in winter (F = 14.459, P = 0.01); ovarian viral RNA levels on day 7 in summer were higher than those on day 7 in winter (F = 8.656, P < 0.001), but there was no significant difference in the viral load at other time points (P > 0.05). Dicer-2 mRNA expression on day 7 in winter was 4.071 times than that on day 7 in summer: the viral load and Dicer-2 expression correlated moderately. Ae. albopictus could still develop and transmit dengue virus in winter in Guangzhou. Mosquitoes under simulated field conditions were more susceptible to DENV-2 than those under simulated laboratory conditions.Author summaryAe. albopictus is the only vector of dengue fever in Guangzhou. Observational data of Ae. albopictus development under natural conditions is important for monitoring vector population expansion, dengue virus transmission, and disease outbreak prevention. We found that Ae. albopictus could still develop in winter in Guangzhou. In addition, the viral load of mosquitoes in the simulated winter environment was lower than that in summer on the 7th day after infection; however, the difference in the viral load of mosquitoes in winter and summer on the 14th day after infection was not statistically significant. Mosquitoes in simulated field conditions were more susceptible to DENV-2 than those in simulated laboratory conditions. The results suggested that Ae. albopictus retains the ability to transmit dengue virus in winter in Guangzhou, and that improved dengue epidemic prevention and control measures are required. The findings of this study represent an important reference for the design of dengue fever control strategies in Guangzhou in response to global climate change.

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Experimental study of the susceptibility of a European Aedes albopictus strain to dengue virus under a simulated Mediterranean temperature regime

Cited by 11 publications

References 28 publications

Arbovirus surveillance: first dengue virus detection in local Aedes albopictus mosquitoes in Europe, Catalonia, Spain, 2015

Arbovirus surveillance: first dengue virus detection in local Aedes albopictus mosquitoes in Europe, Catalonia, Spain, 2015

A Systematic Review: Is Aedes albopictus an Efficient Bridge Vector for Zoonotic Arboviruses?

Effects of Guangzhou seasonal climate change on the development of Aedes albopictus and its susceptibility to DENV-2

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