Experimental study of thermal damage to in vitro skin tissue welding by femtosecond laser

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The influence of femtosecond laser parameters on the degree of thermal denaturation was studied experimentally. The relationship between the degree of thermal denaturation and the characteristic parameters of skin microstructure and the secondary structure of skin tissue proteins in characterizing the degree of thermal damage was analyzed. The results showed the interaction of laser power, laser power, and scanning speed had a significant effect on the degree of thermal denaturation; greater degrees of thermal denaturation were associated with larger second‐order moments of the texture angle of the skin microtissue and smaller entropy values and contrast, indicating a greater degree of thermal damage; and higher peak temperature, the lower peak intensity of Raman spectra, decrease in the percentage area of α‐helix fitted curves and increase in the percentage area of β‐sheet and β‐turn fitted curves indicate that the protein is denatured to a large extent that means thermal damage is large.

Section: Results Analysismentioning

confidence: 99%

“…The changes in the texture and texture feature values of the skin microstructure after welding can be used to characterize the thermal damage to the skin tissue. The microstructural texture grooves of the skin tissue after laser welding were shallow and more chaotically distributed [14].…”

Section: Introductionmentioning

confidence: 99%

Effect of femtosecond laser process parameters on the thermal denaturation degree of skin tissue

Huang,

Jia,

Xia

et al. 2024

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“…The four texture feature parameters of the skin microstructure, such as angular second-order moment, entropy, contrast, and relevance, were extracted by using the same methods as mentioned in our previous research [3,13].…”

Section: Influence Of Process Parameters On the Texture Characteristicsmentioning

confidence: 99%

“…The thermal damage changes during femtosecond laser bonding in vitro skin tissue were evaluated by an orthogonal test. And the results showed that the texture characteristic value of tissue microstructure can be used to judge the degree the thermal damage [3].…”

Section: Introductionmentioning

confidence: 99%

Research on the effect of process parameters on the performance of femtosecond laser‐bonded skin microstructure

Huang

Jia

Xia

et al. 2023

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In this paper, the effect of the femtosecond laser process parameters on the texture characteristics of the microstructure was analyzed with the response surface method. The correspondence between the temperature of skin during laser bonding and microscopic tissue texture characteristics parameters was explored. The results show that the three process parameters of laser power, scanning speed, and scanning times and the interaction between the parameters have different patterns of influence on the four texture characteristics parameters of skin microstructure angular second‐order moments, entropy, contrast, and relevance. Angular second‐order moments and relevance of skin microstructure textures increase with increasing temperature, while entropy values and contrast decrease. It provides another way to evaluate the performance of femtosecond laser‐bonded skin with microstructure.

“…The experiment used fresh pig dorsal skin as the test material, the sample size was 30 mm in length, 20 mm in width, and 2 mm in thickness (0.5 mm in fat layer thickness), and a 20 mm incision was made with a scalpel (thickness direction of the whole layer cut through). Prior to laser scanning, skin samples were soaked in 5% bovine serum albumin solution (BSA) for 20 min to enhance skin tissue bioactivity [21,22]. When the concentration of bovine serum protein is too high, the viscosity of the solution is too high to be treated as a fluid, so the concentration of 5% bovine serum protein was chosen for the test [23].…”

Section: Experimental Process and Materialsmentioning

confidence: 99%

Experimental study on the effect of scanning path on skin tissue properties of femtosecond laser welding

Jia,

Huang,

Chen

et al. 2023

Self Cite

To study the influence pattern of femtosecond laser scanning path on the welding effect of skin tissue, this experiment analyzed the influence of scanning path on the surface morphology, degree of thermal damage, tensile strength, and microstructure of skin samples after skin attachment by designing nine scanning paths to weld skin tissue. The results showed that the skin samples connected by interrupted parallel mattress eversion sewing method with d = 0.2 mm showed no obvious color changes in morphology, the skin samples were connected on both front and back sides, the tensile strength was the highest, reaching 12.80 N/cm2, the thermal damage parameter was low at 1.08 × 10−2, the microstructure had obvious directionality, and the texture was clear and uniformly distributed.