Experimental study on bone defect repair by BMSCs combined with a light-sensitive material: g-C<sub>3</sub>N<sub>4</sub>/rGO

Liang, Jie; Li, Wenhua; Zhuang, Ning; Wen, Sini; Huang, Shijia; Lu, Weizhi; Zhou, Yang; Liao, Gaozu; Bao, Zhenan; Liu, Chenglong

doi:10.1080/09205063.2020.1827923

Cited by 14 publications

(5 citation statements)

References 47 publications

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“…109,110 All animal models successfully compared osteogenesis capability, but the size of the defect was not adequately described to make a pattern for later comparison. However, the sites of the defect could be listed as a cranial defect in 49 experiments, 31,35,39–44,47–50,52,54–56,59–63,65,67,69,71–75,77–81,83,84,86–91,93,94,96,98,100,109,110 limb defect in 25 experiments, 36–38,45,46,51,53,58,64,66,68,70,76,82,85,92,95,97,99,101–103,105–107 or jaw defect in four experiments. 39,57,104,108 These findings may reflect the exposition to functional charges or not during bone repair.…”

Section: Resultsmentioning

confidence: 99%

“…77 experiments were assessed in the manuscripts reviewed. Calcium was associated with graphene in 30 experiments, 37,38,40,41,46,47,58–63,66,77,78,81–83,85,88,89,94–98,102,106,107,110 with 35 experiments presenting the association of graphene with Natural Polymers, 35,39,42–45,47,50–52,56,58,59,62–64,66–71,74,77,81,83,84,86–90,93,100,102,109 and 30 experiments presenting the association of Synthetic Polymers 31,37,48,49,53,54,57,61,65,68,72,73,75,76,79,…”

Section: Resultsmentioning

confidence: 99%

“…31,35,37,39,42–45,47–51,53,55,56,58–64,66,67,69,70,72–75,77,78,81–86,88–91,93,97,98,100,101,103,105,108,109 Graphene only was used in 13 out of 77 manuscripts. 38,46,52,54,57,79,80,82,92,95,99,104,110 Reduced Graphene (from GO) was used in 12 out of 77 manuscripts 40,41,65,68,71,76,87,94,96,102,106,107 and in one out of 77 manuscripts researchers used another graphene type. 36 It was observed that many variations in the Graphene-based material’s concentration can show different results when juxtaposed, leading to the omission of that data from the current review.…”

Section: Resultsmentioning

confidence: 99%

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A scoping review of graphene-based biomaterials for in vivo bone tissue engineering

Achôa

Mattos²,

Clements

et al. 2023

J Biomater Appl

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The growing demand for more efficient materials for medical applications brought together two previously distinct fields: medicine and engineering. Regenerative medicine has evolved with the engineering contributions to improve materials and devices for medical use. In this regard, graphene is one of the most promising materials for bone tissue engineering and its potential for bone repair has been studied by several research groups. The aim of this study is to conduct a scoping review including articles published in the last 12 years (from 2010 to 2022) that have used graphene and its derivatives (graphene oxide and reduced graphene) in preclinical studies for bone tissue regeneration, searching in PubMed/MEDLINE, Embase, Web of Science, Cochrane Central, and clinicaltrials.gov (to confirm no study has started with clinical trial). Boolean searches were performed using the defined key words “bone” and “graphene”, and manuscript abstracts were uploaded to Rayyan, a web-tool for systematic and scoping reviews. This scoping review was conducted based on Joanna Briggs Institute Manual for Scoping Reviews and the report follows the recommendations of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses – Extension for Scoping Reviews (PRISMA- ScR) statement. After the search protocol and application of the inclusion criteria, 77 studies were selected and evaluated by five blinded researchers. Most of the selected studies used composite materials associated with graphene and its derivatives to natural and synthetic polymers, bioglass, and others. Although a variety of graphene materials were analyzed in these studies, they all concluded that graphene, its derivatives, and its composites improve bone repair processes by increasing osteoconductivity, osteoinductivity, new bone formation, and angiogenesis. Thus, this systematic review opens up new opportunities for the development of novel strategies for bone tissue engineering with graphene.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A scoping review of graphene-based biomaterials for in vivo bone tissue engineering

Achôa

Mattos²,

Clements

et al. 2023

J Biomater Appl

View full text Add to dashboard Cite

show abstract

“…Thus, the demand for synthetic scaffold material for bone tissue engineering is increasing for years as they offer a more promising strategy to overcome the conundrum of treating IBDs that plagues orthopaedic surgeons [2]. It has been reported that the combination of appropriate inorganic materials and BMSCs may provide potential for bone regeneration [29]. The ideal injectable bone tissue engineering materials should meet the following characteristics: excellent biocompatibility and biodegradability; capacity of bone induction and bone conduction; proper in vivo degradation rate that matches with the pace of osteogenesis; appropriate structure and porosity for the ingrowth of blood vessels and cell distribution [30,31].…”

Section: Discussionmentioning

confidence: 99%

Application of vancomycin-impregnated calcium sulfate hemihydrate/nano- hydroxyapatite/chitosan injectable hydrogels combined with BMSCs sheet for the treatment of infected bone defects in rabbit model

Wang

Zhi

Liu

et al. 2022

Preprint

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Background Choice of bone substitutes for the therapy of infected bone defects (IBDs) attracts surgeons’ attention for years. However, single-stage bioabsorbable material that provides carriers for antibiotic releasing as well as scaffold for BMSCs sheet need further exploration. Our study was designed to investigate the effect of vancomycin-loaded calcium sulfate hemihydrate/nano-hydroxyapatite/chitosan (CSH/n-HA/CTS) hydrogels combined with BMSCs sheet as bone substitute in the treatment of IBDs. Methods The BMSCs were harvested and cultured into cell sheet. After successful establishment of animal model with chronic osteomyelitis, the 48 New Zealand white rabbits were randomly divided into 4 groups. Animals in group A were treated with thorough debridement as control. Group B were managed with BMSCs sheet. CSH/n-HA/CTS hydrogels were implanted in group C, and group D were treated with CSH/n-HA/CTS + BMSCs sheet. Gross observation and 3D reconstruction of Micro CT were performed to assess the osteogenic and infection elimination ability. Histological staining (Hematoxylin and eosin and Van Gieson) was used to observe inflammatory cell infiltration and formation of collagen fiber 4, 8 ,12 weeks after implantation. Results The bone defect of the control group was not repaired in 12 weeks as chronic osteomyelitis still existed. HE staining showed a large number of inflammatory cell infiltration around the tissue, and VG staining showed no new collagen fiber formation. In the group of BMSCs sheet, though new bone formation was observed by gross observation and Micro-CT scan, the infection was not effectively controlled due to the unfilled cavity. Some neutrophils and only a small amount of collagen fiber could be observed. Both groups of hydrogels and hydrogels/BMSCs achieved satisfactory repair effect and infection control. 3D reconstruction of Micro-CT at 4 weeks showed that the hydrogels/BMSCs sheet group had higher reconstruction efficiency and better bone modeling with normal morphology. HE staining showed little aggregation of inflammatory cells, and VG staining showed a large number of new collagen fibers. Conclusions Our preliminary results proved that compared with single material, the novel antibiotic-impregnated hydrogels acted as superior scaffold for BMSCs sheet and excellent antibiotics vector against infection, which provided a basis for applying tissue engineering technology to the treatment of chronic osteomyelitis.

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“…The researchers co-cultured photosensitive material with BMSC and the cell culture dishes were simultaneously irradiated with blue light for 30 min per day. The spectroscopic results showed that photocurrent generation from π-π* orbitals in visible light could provide a stronger driving force for osteogenesis [ 197 ].…”

Section: Engineered Bone Microenvironmentmentioning

confidence: 99%

Microenvironment-targeted strategy steers advanced bone regeneration

Shen

Wang

Yin

et al. 2023

Materials Today Bio

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Experimental study on bone defect repair by BMSCs combined with a light-sensitive material: g-C₃N₄/rGO

Cited by 14 publications

References 47 publications

A scoping review of graphene-based biomaterials for in vivo bone tissue engineering

A scoping review of graphene-based biomaterials for in vivo bone tissue engineering

Application of vancomycin-impregnated calcium sulfate hemihydrate/nano- hydroxyapatite/chitosan injectable hydrogels combined with BMSCs sheet for the treatment of infected bone defects in rabbit model

Microenvironment-targeted strategy steers advanced bone regeneration

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Experimental study on bone defect repair by BMSCs combined with a light-sensitive material: g-C3N4/rGO

Cited by 14 publications

References 47 publications

A scoping review of graphene-based biomaterials for in vivo bone tissue engineering

A scoping review of graphene-based biomaterials for in vivo bone tissue engineering

Application of vancomycin-impregnated calcium sulfate hemihydrate/nano- hydroxyapatite/chitosan injectable hydrogels combined with BMSCs sheet for the treatment of infected bone defects in rabbit model

Microenvironment-targeted strategy steers advanced bone regeneration

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Experimental study on bone defect repair by BMSCs combined with a light-sensitive material: g-C₃N₄/rGO