Experimental study on in vitro tumor cell killing by focused bi-frequency ultrasound activated hematoporphyrin derivatives

Shang, Zhenfeng; Ren, Xu; Zhang, J.; Zhu, Xue-Xue; Qi, Hao; Liu, Q.; Zhang, K.; Wu, Shuicai; Zeng, Yiming

doi:10.1007/bf03178576

Cited by 3 publications

(2 citation statements)

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“…The activation of the T-cell system in tumor tissue by up-regulation of prothymosin alpha, cytotoxic T lymphocyte-associated protein 2 alpha, and the T-cell specific GTPase [27] could be observed. The up-regulation of annexin A1-lipocortin 1 in the tumors treated with short-pulse focused ultrasound beams and the up-regulation of the CD14 antigen and other genes support the hypothesis that application of short-pulse focused ultrasound beams does induce apoptosis [28,29]. The other up-regulated genes induce a variety of cellular processes, such as genes regulating the replication of DNA, transcription, and protein biosynthesis or regulation of the cell cycle and cell proliferation.…”

Section: Discussionsupporting

confidence: 54%

Gene Expression Profiles, Histologic Analysis, and Imaging of Squamous Cell Carcinoma Model Treated with Focused Ultrasound Beams

Hundt

Yuh

Bednarski

et al. 2007

American Journal of Roentgenology

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OBJECTIVE. The purpose of our study was to evaluate the effect of short-pulse high-intensity focused ultrasound (HIFU) on inducing cell death in a head and neck cancer model (SCCVII [squamous cell carcinoma]) compared with continuous HIFU to get a better understanding of the biologic changes caused by HIFU therapy.MATERIALS AND METHODS. HIFU was applied to 12 SCCVII tumors in C3H/Km mice using a dual sonography system (imaging, 6 MHz; therapeutic, 1 MHz). A continuous HIFU mode (total time, 20 seconds; intensity, 6,730.6 W/cm 2 ) and a short-pulse HIFU mode (frequency, 0.5Hz; pulse duration, 50 milliseconds; total time, 16.5 minutes; intensity, 134.4 W/cm 2 ) was applied. Three hours later, MR images were obtained on a 1.5-T scanner. After imaging, the treated and untreated control tumor tissue samples were taken out for histology and oligonucleotide microarray analysis.RESULTS. Prominent changes were observed in the MR images in the continuous HIFU mode, whereas the short-pulse HIFU mode showed no discernible changes. Histology (H and E, TUNEL [terminal deoxynucleotidyl transferase-mediated dUTP {deoxyuridine triphosphate} nick-end labeling], and immunohistochemistry) of the tumors treated with the continuous HIFU mode revealed areas of significant necrosis. In the short-pulse HIFU mode, the H and E staining showed multifocal areas of coagulation necrosis. TUNEL staining showed a high apoptotic index in both modes. Gene expression analysis revealed profound differences. In the continuous HIFU mode, 23 genes were up-regulated (> twofold change) and five genes were down-regulated (< twofold change), and in the short-pulse HIFU mode, 32 different genes were up-regulated and 16 genes were down-regulated.CONCLUSION. Genomic analysis might be included when investigating tissue changes after interventional therapy because it offers the potential to find molecular targets for imaging and therapeutic applications. rocedures using guided external energy deposition such as radiofrequency ablation, cryoablation, and high-intensity focused ultrasound (HIFU) are increasing in clinical use. HIFU technology makes possible the deposition of controlled energy doses deep into soft tissue. Despite the expanding use of these procedures, little is known about the changes in gene expression and the relationship to the doses of energy exposure. Knowledge about the changes in gene expression induced by external energy deposition can be useful in characterizing changes in tissues morphology and design of new therapies. For example, in studies related to defining cellular changes during radiation therapy, it has been shown that immediate and early gene expression is induced after exposure of tissue to various stresses, and these genes can mediate cell proliferation, differentiation, survival, and even cell death [1][2][3]. These investigations led to new therapy options being evaluated clinically [4].Currently, only imaging and histologic analysis have been used to characterize tissue during an interventional radiologic procedure, includ...

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Section: Discussionsupporting

confidence: 54%

Gene Expression Profiles, Histologic Analysis, and Imaging of Squamous Cell Carcinoma Model Treated with Focused Ultrasound Beams

Hundt

Yuh

Bednarski

et al. 2007

American Journal of Roentgenology

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“…Hematoporphyrin is an endogenous porphyrin produced by hydrolysis of hemoglobin. It has been reported that hematoporphyrin could inhibit the proliferation of tumor cells 35–37. The level of hematoporphyrin in EPS1-1 group was higher than the Model group, showing that EPS1-1 could induce the production of hematoporphyrin to suppress the growth of colorectal cancer cells.…”

Section: Discussionmentioning

confidence: 86%

<p>Metabonomic Variation of Exopolysaccharide from <em>Rhizopus nigricans</em> on AOM/DSS-Induced Colorectal Cancer in Mice</p>

Lü¹,

Wang²,

Ji³

et al. 2019

OTT

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BackgroundColorectal cancer (CRC), which occurs at the junction of the rectum and sigmoid colon, is a common malignancy associated with poor prognosis and high mortality worldwide. The exopolysaccharide (EPS1-1), isolated from the fermentation broth of Rhizopus nigricans (R. nigricans), has been reported to possess anti-CRC properties. However, the metabolic alterations caused by azoxymethane (AOM) and dextran sulfate sodium (DSS) are still unknown.MethodsIn the present study, a mice colon cancer model was established by treatment with AOM/DSS. LC-MS/MS-based metabolomics studies were performed to analyze metabolic alterations at the tissue level. Partial least squares discriminant analysis (PLS-DA) was used to identify differentially expressed metabolites.ResultsNineteen distinct metabolites were identified that were associated with disruptions in the following pathways: biosynthesis of unsaturated fatty acids, pyrimidine metabolism, phenylalanine metabolism, fatty acid metabolism, folate biosynthesis, and inositol phosphate metabolism. Furthermore, six significantly altered metabolites were involved in these six pathways. Compared with the Model group, the expression of cytosine, deoxyuridine, 20-hydroxy-leukotriene E4, and L-homocysteic acid was lower, whereas that of 2-dehydro-3-deoxy-6-phospho-D-gluconic acid and hematoporphyrin was higher in the EPS1-1 group.ConclusionThe results of multivariate statistical analysis demonstrate a promising application of the above metabolites by EPS1-1 in CRC therapy. Deeper understanding of the related mechanism warrants further investigation.

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A comparative study on generating hydroxyl radicals by single and two-frequency ultrasound with gold nanoparticles and protoporphyrin IX

Tabatabaei

Rajabi

Nassirli

et al. 2019

Australas Phys Eng Sci Med

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Experimental study on in vitro tumor cell killing by focused bi-frequency ultrasound activated hematoporphyrin derivatives

Abstract: The method of killing tumor cells by focused bi-frequency ultrasound activated hematoporphyrin derivatives is presented and evaluated in this paper.

Cited by 3 publications

References 4 publications

Gene Expression Profiles, Histologic Analysis, and Imaging of Squamous Cell Carcinoma Model Treated with Focused Ultrasound Beams

Gene Expression Profiles, Histologic Analysis, and Imaging of Squamous Cell Carcinoma Model Treated with Focused Ultrasound Beams

<p>Metabonomic Variation of Exopolysaccharide from <em>Rhizopus nigricans</em> on AOM/DSS-Induced Colorectal Cancer in Mice</p>

A comparative study on generating hydroxyl radicals by single and two-frequency ultrasound with gold nanoparticles and protoporphyrin IX

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