Experimental systemic virulence of oral Candida dubliniensis isolates in comparison with Candida albicans, Candida tropicalis and Candida krusei

Koga-Ito, Cristiane Yumi; Komiyama, Edson Yukio; Martins, Clélia Aparecida de Paiva; Vasconcellos, T. C.; Jorge, Antônio Olavo Cardoso; Carvalho, Yasmin Rodarte; Prado, Renata Falchete do; Balducci, Ivan

doi:10.1111/j.1439-0507.2010.01899.x

Cited by 15 publications

(15 citation statements)

References 23 publications

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“…This is consistent with results using the i.v. model of systemic candidiasis, with high mortality observed with C. albicans and C. tropicalis and low mortality with C. glabrata, C. dubliniensis, and C. parapsilosis (43,44). The exception is C. krusei, which causes variable mortality in disseminated i.v.…”

Section: Discussionmentioning

confidence: 99%

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Morphology-Independent Virulence of Candida Species during Polymicrobial Intra-abdominal Infections with Staphylococcus aureus

et al. 2016

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c Intra-abdominal polymicrobial infections cause significant morbidity and mortality. An experimental mouse model of Candida albicans-Staphylococcus aureus intra-abdominal infection (IAI) results in 100% mortality by 48 to 72 h postinoculation, while monomicrobial infections are avirulent. Mortality is associated with robust local and systemic inflammation without a requirement for C. albicans morphogenesis. However, the contribution of virulence factors coregulated during the yeast-tohypha transition is unknown. This also raised the question of whether other Candida species that are unable to form hyphae are as virulent as C. albicans during polymicrobial IAI. Therefore, the purpose of this study was to evaluate the ability of non-albicans Candida (NAC) species with various morphologies and C. albicans transcription factor mutants (efg1/efg1 and cph1/cph1) to induce synergistic mortality and the accompanying inflammation. Results showed that S. aureus coinoculated with C. krusei or C. tropicalis was highly lethal, similar to C. albicans, while S. aureus-C. dubliniensis, S. aureus-C. parapsilosis, and S. aureus-C. glabrata coinoculations resulted in little to no mortality. Local and systemic interleukin-6 (IL-6) and prostaglandin E 2 (PGE 2 ) levels were significantly elevated during symptomatic and/or lethal coinfections, and hypothermia strongly correlated with mortality. Coinoculation with C. albicans strains deficient in the transcription factor Efg1 but not Cph1 reversed the lethal outcome. These results support previous findings and demonstrate that select Candida species, without reference to any morphological requirement, induce synergistic mortality, with IL-6 and PGE 2 acting as key inflammatory factors. Mechanistically, signaling pathways controlled by Efg1 are critical for the ability of C. albicans to induce mortality from an intra-abdominal polymicrobial infection. I ntra-abdominal infection (IAI) is a broad term used to describe numerous infections caused by the invasion of microorganisms into the abdominal cavity. These infections can range from appendicitis to complicated peritonitis and are initiated by microbes entering the abdominal cavity through a variety of routes, including bowel perforation, laparotomy, intestinal hernias, and insertion of medical devices such as peritoneal catheters (reviewed in reference 1). These infections often are polymicrobial in nature, which complicates diagnosis and treatment. Accordingly, infections with multiple microbes can result in amplified pathogenicity, also known as "infectious synergism" (2-4), which may accelerate disease progression or protect microbes from host responses (5), resulting in higher morbidity and mortality (6, 7).Polymicrobial IAIs involving fungi result in mortality rates between 50 and 75%, compared to 10 to 30% for polymicrobial bacterial infections (8-11). The majority of polymicrobial fungal IAIs are caused by Candida albicans (12, 13). However, IAIs caused by non-albicans Candida (NAC) species are becoming more common (14). C. al...

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Section: Discussionmentioning

confidence: 99%

“…The exception is C. krusei, which causes variable mortality in disseminated i.v. models of systemic candidiasis (43,44). Note that all of these species cause mortality clinically, mostly in immunocompromised or neonatal patients, and the incidence of candidemia due to NAC has increased in the past several years (45).…”

Section: Discussionmentioning

confidence: 99%

Morphology-Independent Virulence of Candida Species during Polymicrobial Intra-abdominal Infections with Staphylococcus aureus

et al. 2016

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“…Using immunocompetent mice, the IV challenge model has been used to compare the virulence of different Candida species [97–99, 107, 111–114]. C. albicans is clearly the most virulent species [97, 98, 111, 112, 114], followed closely by C. tropicalis [97, 98, 111, 112, 114].…”

Section: Experimental Models Of Candida Infectionmentioning

confidence: 99%

“…C. albicans is clearly the most virulent species [97, 98, 111, 112, 114], followed closely by C. tropicalis [97, 98, 111, 112, 114]. In contrast, C. krusei and C. parapsilosis were unable to kill the infected animals, even at high inoculum levels, and fungi were eventually cleared from the host [98, 111, 114]. …”

Section: Experimental Models Of Candida Infectionmentioning

confidence: 99%

Hosting Infection: Experimental Models to AssayCandidaVirulence

MacCallum

2012

International Journal of Microbiology

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Although normally commensals in humans, Candida albicans, Candida tropicalis, Candida parapsilosis, Candida glabrata, and Candida krusei are capable of causing opportunistic infections in individuals with altered physiological and/or immunological responses. These fungal species are linked with a variety of infections, including oral, vaginal, gastrointestinal, and systemic infections, with C. albicans the major cause of infection. To assess the ability of different Candida species and strains to cause infection and disease requires the use of experimental infection models. This paper discusses the mucosal and systemic models of infection available to assay Candida virulence and gives examples of some of the knowledge that has been gained to date from these models.

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“…albicans is compounded by continuous intake of antibiotic drugs, anticancer agents, oral contraceptives, HIV protease inhibitors and compromised immune system (Koga-Ito et al, 2011). Plant extracts have been shown to have anticandidal activity (Shai et al,2008(Shai et al, , 2009).…”

Section: Introductionmentioning

confidence: 99%

Anticandidal activity of cell extracts from 13 probiotic Lactobacillus strains and characterisation of lactic acid and a novel fatty acid derivative from one strain

et al. 2014

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This study investigated the anti-Candida activity of methanol extracts from freezedried probiotic cells and the isolation of some constituents in the extracts. The MIC values of the probiotic methanol cell extracts against Candida albicans ranged between 1.25-5 mg/ml after 48 h of incubation. However, Lactococcus latics subsp. lactis strain X and Lactobacillus casei strain B extracts had an MIC of 10 mg/ml after 48 h of incubation. The extracts had fungistatic rather than fungicidal activity. These extracts had a much higher antifungal activity than antifungal compounds isolated from the growth medium by many other authors. 2This indicates that probiotics may also release antifungal compounds in their cells that could contribute to a therapeutic effect. Lactic acid (1) and 6-O-(α-D-glucopyranosyl)-1,6-di-Opentadecanoyl-α-D-glucopyranose a novel fatty acid derivative (2) were isolated from methanol probiotic extracts and the structure of these compounds were elucidated by using NMR (1 and 2D) and mass spectrometry (MS).

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Experimental systemic virulence of oral Candida dubliniensis isolates in comparison with Candida albicans, Candida tropicalis and Candida krusei

Cited by 15 publications

References 23 publications

Morphology-Independent Virulence of Candida Species during Polymicrobial Intra-abdominal Infections with Staphylococcus aureus

Morphology-Independent Virulence of Candida Species during Polymicrobial Intra-abdominal Infections with Staphylococcus aureus

Hosting Infection: Experimental Models to AssayCandidaVirulence

Anticandidal activity of cell extracts from 13 probiotic Lactobacillus strains and characterisation of lactic acid and a novel fatty acid derivative from one strain

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