Experimental Transmission of Enzootic Intranasal Tumors of Goats

Heras, M. De las; Jalón, J. A. García de; Minguijón, E.; Gray, E. W.; Dewar, P.; Sharp, J. M.

doi:10.1177/030098589503200103

Cited by 46 publications

(38 citation statements)

References 5 publications

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“…Viral-like particles, compatible with a type D retrovirus, were isolated from nasal fluid, and neoplastic tissue and successful experimental transmission of EIT from goat to goat confirmed this hypothesis. 1,3,20 In spontaneous disease, the infection occurs through the respiratory route, and the incubation time is so long that the clinical disease is found in animals that are approximately 1 year old. 23 The complete histogenesis of EIT still remains unknown.…”

Section: Origin Of Enzootic Intranasal Tumor In the Goat (Capra Hircumentioning

confidence: 99%

Origin of Enzootic Intranasal Tumor in the Goat (Capra hircus): A Glycohistochemical Approach

et al. 2001

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Abstract. Enzootic intranasal tumor (EIT) appears glandular in type and has recently been classified as an adenocarcinoma of low malignancy. The aim of this study was to characterize the secretion of surface glycoconjugates (GCs) in EIT and in normal respiratory and olfactory mucosae of the goat by means of conventional and lectin histochemistry, in order to shed light on the histogenesis of EIT. Morphologic and ultrastructural investigations showed two growth types of EIT: i.e., tubular and papillary patterns. Conventional histochemistry revealed the presence of neutral and carboxylated GCs in the olfactory glands and in the tubular part of EIT, as well neutral and sulphated GCs in the respiratory glands and in the papillary part of EIT, suggesting that the papillary pattern tumor arises from the respiratory glands, whereas the tubular portion of EIT arises from the olfactory glands. Lectin histochemistry gave further information on the expressed GCs.

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Section: Origin Of Enzootic Intranasal Tumor In the Goat (Capra Hircumentioning

confidence: 99%

Origin of Enzootic Intranasal Tumor in the Goat (Capra hircus): A Glycohistochemical Approach

et al. 2001

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“…Each of these tumours has been shown consistently to contain a retrovirus with morphological, biochemical and immunological properties similar to type D retroviruses and cell-free tumour extracts can be used to reproduce these diseases experimentally (Cohrs, 1953;Martin et al, 1976;Verwoerd et al, 1980;DeMartini et al, 1988;De las Heras et al, 1995). * Author for correspondence.…”

Section: Introductionmentioning

confidence: 99%

Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus

Palmarini

Dewar

Heras

et al. 1995

Journal of General Virology

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Sheep pulmonary adenomatosis (SPA) is a naturally occurring contagious lung tumour of sheep which has been associated aetiologically with a type D-and Brelated retrovirus (Jaagsiekte retrovirus; JSRV). To improve understanding of the aetio-pathogenesis of SPA, the distribution and the sites of JSRV replication in sheep with naturally or experimentally induced SPA or in unaffected controls were identified. New immunological reagents were produced and a blocking enzymelinked immunosorbent assay (B-ELISA) and an immunohistochemical technique for the detection of JSRV major capsid protein at the tissue and cellular levels were developed. JSRV was detected only in the respiratory tract of sheep affected by pulmonary adenomatosis and specifically in the transformed epithelial cells of the alveoli of SPA-affected sheep.

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“…Enzootic nasal tumor virus (ENTV) is a simple retrovirus that is transmitted horizontally and induces nasal adenocarcinoma in sheep (4,10) and goats (8,9). ENTV can be found in the nasal fluid of animals with intranasal tumors, which eventually progress and cause severe cranial deformations and respiratory blockage, resulting in death (41).…”

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Mechanism of Cell Entry and Transformation by Enzootic Nasal Tumor Virus

Dirks

Duh

Sharath³

et al. 2002

J Virol

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Enzootic nasal tumor virus (ENTV) induces nasal epithelial cancer in infected sheep, but it is a simple retrovirus lacking a known oncogene. ENTV is closely related to jaagsiekte sheep retrovirus (JSRV), which also causes cancer in sheep but in the epithelial cells of the lower airways and alveoli. Here we show that as with JSRV, the envelope (Env) protein of ENTV can transform cultured cells and thus is likely to be responsible for oncogenesis in animals. In addition, the ENTV Env protein mediates virus entry using the same receptor as does JSRV Env, the candidate tumor suppressor Hyal2. However, ENTV Env mediates entry into cells from a more restricted range of species than does JSRV, and based on this finding we have identified amino acid regions in the Env proteins that are important for virus entry. Also, because ENTV does not efficiently use human Hyal2 as a receptor, we cloned the ovine Hyal2 cDNA and show that the encoded protein functions as an efficient receptor for both ENTV and JSRV. In summary, although ENTV and JSRV use the same cell surface receptor for cell entry and apparently transform cells by the same mechanism, they induce cancer in different tissues of infected sheep, indicating that oncogenesis is regulated at some other level. The transcriptional regulatory elements in these viruses are quite different, indicating that tissue-specific oncogenesis is likely regulated at the level of viral gene expression. Enzootic nasal tumor virus (ENTV) is a simple retrovirusthat is transmitted horizontally and induces nasal adenocarcinoma in sheep (4, 10) and goats (8, 9). ENTV can be found in the nasal fluid of animals with intranasal tumors, which eventually progress and cause severe cranial deformations and respiratory blockage, resulting in death (41). ENTV is closely related to jaagsiekte sheep retrovirus (JSRV) (Ͼ95% overall amino acid similarity) (4), which is the causative agent of ovine pulmonary adenocarcinoma (also called sheep pulmonary adenomatosis or jaagsiekte) (30). Unlike tumors caused by ENTV, which arise from nasal epithelial cells, JSRV-induced tumors arise from epithelial cells in the lower airway, including type II alveolar and bronchiolar epithelial cells (27). The mechanism of oncogenesis by ENTV and its relationship to that caused by JSRV are unknown. Both of these viruses are present in many countries worldwide and have a significant economic and animal health impact. In addition, the disease induced by JSRV exhibits histological features similar to those of many human pulmonary adenocarcinomas, including bronchioloalveolar carcinoma (28, 33). Thus, study of adenocarcinoma induced by JSRV and ENTV may provide insights into the etiology of human lung cancer. While ENTV and JSRV do not appear to cause lung cancer in humans having occupational exposure to these viruses, a recent study shows that antiserum directed against the JSRV capsid protein cross-reacts with 30% of human pulmonary adenocarcinoma samples but not with nontumorous lung lesions, normal lung tissue, or many adenoc...

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Experimental Transmission of Enzootic Intranasal Tumors of Goats

Cited by 46 publications

References 5 publications

Origin of Enzootic Intranasal Tumor in the Goat (Capra hircus): A Glycohistochemical Approach

Origin of Enzootic Intranasal Tumor in the Goat (Capra hircus): A Glycohistochemical Approach

Epithelial tumour cells in the lungs of sheep with pulmonary adenomatosis are major sites of replication for Jaagsiekte retrovirus

Mechanism of Cell Entry and Transformation by Enzootic Nasal Tumor Virus

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