Experimental Unilateral Spermatic Cord Torsion: The Effect of Polypolymerase Enzyme Inhibitor on Histopathological and Biochemical Changes in the Early and Late Periods in the Ipsilateral and Contralateral Testicles

Kar, Aytan; Özden, Ender; Yakupoğlu, Yarkın Kamil; Kefeli, Mehmet; Sarıkaya, Şaban; Yılmaz, Ali Faik

doi:10.1016/j.urology.2010.03.074

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…Our histopathological results support our biochemical results and agree with previous studies in which one‐sided testis torsion was applied experimentally and opposite testis damage was researched (Kar et al., ). We found severe inflammatory infiltration in the torsioned left testes at 1, 2 and 3 months for the T/D+, Zn− group, and normal morphology of the tubular structure was lost.…”

Section: Discussionsupporting

confidence: 92%

Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion

et al. 2016

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The purpose of this study was to examine potential long-term post-torsion changes that can occur in the histopathology, biochemistry and spermatogenesis of both torsioned and nontorsioned opposite testes. The study also determines the effect of zinc (Zn) administration on the testicular torsion/detorsion (T/D) damage on both testes. Forty-eight male rats, divided equally into eight groups: (SHAM), (SHAM+,Zn+), (T/D+, Zn- 1 month), (T/D+,Zn- 2 months), (T/D+,Zn- 3 months), (T/D+,Zn+ 1 months), (T/D+,Zn+ 2 months), (T/D+,Zn+ 3 months), have been used. Drug administration was carried out by adding 100 μg (0.016 ml/rat) Zn per rat to drinking water in related groups. Testicular damage decreased superoxide dismutase (SOD) and glutathione (GSH) and increased malondialdehyde (MDA) in the testis tissues of rats, while Zn administration increased SOD and GSH and decreased MDA in the testis tissues in comparison with the SHAM group. The beneficial effect of zinc sulphate was more evident on the nonrotated testis than the rotated testis. In the histopathological study, a significant decrease in torsion and detorsion injuries was observed in the treatment groups compared to the torsion and detorsion groups. We found a protective effect of zinc sulphate on oxidative stress as a result of T/D injuries in rats, especially for the nonrotated testis; results were supported histopathologically.

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Section: Discussionsupporting

confidence: 92%

Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion

et al. 2016

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“…Studies in human beings have shown an ipsilateral testicular atrophy after testicular rescue (Sessions et al, 2003;Cimador et al, 2007). Animal studies have found a significant decrease in ipsilateral testicular spermatogenesis after testicular salvage (Erol et al, 2010;Kar et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Psoralea corylifolia protects against testicular torsion/detorsion-induced ischemia/reperfusion injury

Wei

Zhou

2011

Journal of Ethnopharmacology

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“…showed that nicotinamide as a PARP inhibitor may successfully decrease the tissue damage due to ischaemia–reperfusion injury in the early and late periods in both the ipsilateral and contralateral testicles (Kar et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…Administration of PARP inhibitors before reperfusion in a torsion-detorsion model of testis indicates a potential for decreasing the long-term histological damage that occurs after testicular torsion. Kar et al showed that nicotinamide as a PARP inhibitor may successfully decrease the tissue damage due to ischaemiareperfusion injury in the early and late periods in both the ipsilateral and contralateral testicles (Kar et al 2010).…”

Section: Discussionmentioning

confidence: 99%

The efficiency of Poly(ADP‐Ribose) Polymerase (PARP) cleavage on detection of apoptosis in an experimental model of testicular torsion

Koşar

Tuncer

Uğuz

et al. 2015

Int J Experimental Path

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The aim of this study was to evaluate the histopathological and apoptotic changes occurring in the rat ipsilateral and contralateral testes, after experimental spermatic cord torsion, and to explore and the role of poly(ADP-ribose) polymerase (PARP) cleavage in testicular torsion-detorsion injury. A total of 37 Wistar albino rats were subjected to 720° unilateral spermatic cord torsion for 1, 2 and 4 h, followed by 4-h reperfusion, or else to a sham operation (control group). Histology of the testicle was evaluated using haematoxylin-eosin (H&E) staining and Johnsen's scoring system. Germ cell apoptosis was evaluated via active caspase-3 immunostaining, and PARP expression levels were evaluated via Western blotting. The mean Johnsen's tubular biopsy scores (JTBS) of the ipsilateral testicles were lower for all torsion groups than for the controls (P < 0.05), but the JTBS of the contralateral testicles were only lower in the 4-h torsion group (P < 0.05). The mean apoptosis score (AS) of the ipsilateral and contralateral testicles was significantly higher in the torsion groups than in the sham group. AS increased correlatively with torsion time, in both testicles. The effect of testicular torsion on PARP cleavage was time dependent, with the highest effect observed after 4 h of testicular torsion (P < 0.05). Testicular torsion caused time-dependent histological changes, apoptosis and increases in PARP cleavage. Our results suggest that testicular torsion-detorsion injury caused cell damage and germ cell apoptosis that apparently involved cleavage of PARP. Increased PARP cleavage could, in turn, lead to enhanced apoptosis.

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Experimental Unilateral Spermatic Cord Torsion: The Effect of Polypolymerase Enzyme Inhibitor on Histopathological and Biochemical Changes in the Early and Late Periods in the Ipsilateral and Contralateral Testicles

Cited by 8 publications

References 26 publications

Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion

Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion

Psoralea corylifolia protects against testicular torsion/detorsion-induced ischemia/reperfusion injury

The efficiency of Poly(ADP‐Ribose) Polymerase (PARP) cleavage on detection of apoptosis in an experimental model of testicular torsion

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