Experimental Vibrio harveyi infections in Penaeus vannamei larvae

Robertson, Peter A.; Calderón, Jorge; Carrera, Luis Gómez; Stark, J.R.; Zherdmant, M.; Austin, Brian

doi:10.3354/dao032151

Cited by 89 publications

(84 citation statements)

References 9 publications

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“…On the other hand, a noticeable potent growth inhibition (MIC = 0.3 -0.6 mM for Pen-3a) associated with slow killing of the target bacteria (10% of bacteria are still alive after a 24-h incubation) was observed on Aerococcus 6iridans, which is reported to be highly pathogenic in crabs and lobsters, in which it induces rapid septicemia and subsequent death [37]. Finally, penaeidins were found to display little activity against Gram-negative strains, including the Vibrio species commonly responsible for many crustacean bacterial infections [38,39]. Despite better efficiency of Pen-3a on most of the strains tested, similar activity spectra and inhibition mechanisms are observed for both Pen-2 and -3a (at concentrations above the MIC, sensitive strains are killed or inhibited with identical time courses).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda)

Destoumieux¹,

Muñoz²,

Bulet

et al. 2000

CMLS, Cell. Mol. Life Sci.

174

105

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Abstract. The production of antimicrobial peptides rep-This review presents findings on a family of antimicrobial resents a first-line host defense mechanism of innate peptides, named penaeidins, isolated from the shrimp Penaeus 6annamei. Their structure and antimicrobial immunity that is widespread in nature. Only recently properties as well as their immune function will be such effectors were isolated in crustacean species, whereas discussed through analyses of penaeidin gene expression numerous antimicrobial peptides have been characterized from other arthropods, both insects and chelicerates. and peptide distribution upon microbial challenge.

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Section: Antibacterial Activitymentioning

confidence: 99%

Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda)

Destoumieux¹,

Muñoz²,

Bulet

et al. 2000

CMLS, Cell. Mol. Life Sci.

174

105

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“…Karunasagar et al (1994) señalaron una menor sobrevivencia en los subestadios larvarios de P. monodon cuando fueron infectados con una cepa de V. harveyi resistente a cloranfenicol y eritromicina, comparados con aquellos que fueron infectados con una cepa silvestre de V. harveyi (dosis de 10 3 y 10 5 UFC/ml, respectivamente). Robertson et al (1998) reportaron infección en los subestadios larvarios de L. vannamei con V. harveyi a una dosis de 10 5 UFC/ml, la cual fue capaz de producir bioluminicencia y una enfermedad larval denominada como bolitas negras. Por otra parte, Kannapiran et al (2009) y SotoRodríguez et al (2012 sugirieron que V. harveyi es responsable de diversos problemas en camarón, siendo el síndrome de enfermedad de las patas rojas uno de ellos.…”

Section: Resultados Y Discusiónunclassified

“…Las especies que conforman al género Vibrio, originan trastornos a las larvas de camarón, los cuales pueden llevar a la muerte de las mismas (Widanarni et al, 2003;Austin y Zhang, 2006;Ravi et al, 2007). Al infectar subestadios larvales de L. vannamei con V. harveyi (10 5 UFC/ml), Robertson et al (1998) determinaron que esta dosis fue suficiente para generar el trastorno conocido como bolitas negras, cuyos síntomas son degradación de tejido hepatopáncreatico, nado lento, reducción en la alimentación, y retardo en el desarrollo larval, además de la bioluminiscencia que puede llegarse a percibir con esta especie de Vibrio. Estos trastornos pudieron propiciar la mortalidad de los subestadios larvarios empleados es esa investigación.…”

Section: Introductionunclassified

Vibrio harveyi effect under survival of Litopenaeus vannamei larvae

2013

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Recibido 30 abril 2013; aceptado 29 mayo 2013Resumen Los cultivos acuícolas son de gran relevancia en la alimentación humana, pero crean un medio ambiente artificial que promueve el crecimiento de diferentes especies de bacterias. Las especies del género Vibrio son bacterias de la microflora normal de los camarones peneidos y son también agentes patógenos oportunistas que pueden tomar ventaja de éstos cambios ecológicos generados en los cultivos acuícolas causando diferentes enfermedades, sobrevivencias bajas y pérdidas económicas en la producción de camarón. El objetivo de la presente investigación fue determinar la variación en la sensibilidad, de diferentes subestadios larvarios ), siendo la última dosis la que presentó los valores más bajos de sobrevivencia. Además, se observó que los subestadios larvales y en el de postlarva 1 fueron más resistentes a V. harveyi al aumentar la edad de los mismos. Esta información tiene gran significado para la industria acuícola, ya que les permite generar estrategias que disminuyan los efectos de V. harveyi y que les permita mejorar el crecimiento y sobrevivencia de las larvas y de postlarva 1 del camarón. Palabras clave: Litopenaeus vannamei, camarón, infección, sobrevivencia, Vibrio harveyi. AbstractThe culture of aquatic organisms show a high relevance in the human feeding and the culture activities can create artificial conditions that increase the growth and selection of specific bacteria. Vibrio species are normal bacteria's from microflora of penaeid shrimp, those are opportunistic pathogens that can take advantage of the ecological changes generated for the culture of aquatic organisms and which may cause diseases, low survival and economic losses in the shrimp production. The aim of this research was to determine the variation in the survival of different larval substages (nauplius, zoea I-III, mysis I-III) and postlarvae 1, of Pacific white shrimp Litopenaeus vannamei exposed at three doses [10 ), where higher doses show the lowest values of survival. Larval substages and postlarvae 1 of shrimp showed sensitivity associate to the increase of Vibrio doses and this sensitivity decreased with the growth of larval substages and postlarvae 1. This information has high significance for the fisheries and aquaculture industry, which help to generate strategies to reduce the effects of V. harveyi with positive effect in growth and survival of the shrimp larvae and postlarvae 1.

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“…Bowser et al (1981) reported vacuole formation in hepatopancreas which resulted after the intra-hemocoelic injection of Vibrio spp. Pathogenecity of Vibrio harveyi was studied by Robertson et al (1998) in Penaeus vannamei larvae and the results showed necrotic bundles in hepatopancreas of the infected animal. The present study also supports previous studies with necrotic bundles found scattered throughout the hepatopancreas of the shell-diseased N. mederi.…”

Section: Discussionmentioning

confidence: 99%

Shell disease of Neoepisesarma mederi crabs and its associated secondary infections

Sharmila¹,

Ravich²,

ran³

et al. 2013

Afr. J. Microbiol. Res.

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Shells of crabs are infected with a high prevalence of diseases than any other crustacean. Bacteria, viruses, fungi and several other pathogens influence higher percentage of shell disease in crabs. Infections in crab affected growth and metabolism and it may also lead to death. This work deals with the microscopic examination of shell diseased crabs with reference to its variation in hemocytes count, histopathology and biochemical estimation. The total heterotrophic bacterial (THB) population in normal shell was found to be 5.15 x 10 4 ± 0.09 Colony forming unit (CFU) ml -1 and THB load was noted to be slightly increased than in normal shell and the count was 5.95 x 10 4 ± 0.12 CFU ml -1 in the infected shell. The current findings suggest a positive correlation between bacterial shell disease and the secondary infection affecting hemolymph (total hemocyte count), proximate composition (protein, carbohydrate and lipid) and histopathology of the animal (gills, hepatopancreas and heart). The total hemocyte count and differential count were experimented which showed lower number of total hemocytes in infected crabs while no highly accountable difference was found in differential count. Hematology studies reported total hemocyte as 9.93 x10 6 ± 0.07 cells ml -1 in normal and 6.525 x 10 6 ± 0.12 cells ml -1 in shell diseased hemolymph. The level of protein, carbohydrate and lipid contents suffered a mild alteration in crabs with bacterial shell disease. Histopathology of gills had shown variations such as dilated lamellae and hemocyte accumulation (nodules), hepatopancreas showed mild difference with increased necrosis, appearance of hemocytic nodules in the hemal spaces and heart with very mere infection while no reportable changes were accomplished in muscle tissue.

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Experimental Vibrio harveyi infections in Penaeus vannamei larvae

Cited by 89 publications

References 9 publications

Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda)

Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda)

Vibrio harveyi effect under survival of Litopenaeus vannamei larvae

Shell disease of Neoepisesarma mederi crabs and its associated secondary infections

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