Experimentelle studien an der symbiose von Coptosoma scutellatum
Geoffr. (Hem. heteropt.)

Müller, Hj.

doi:10.1007/bf00407170

Cited by 31 publications

(45 citation statements)

References 6 publications

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“…In a number of pentatomomorphan bugs, symbiont-free insects exhibit retarded growth, nymphal mortality, and/or sterility (1,13,17,22,24), suggesting that their symbionts play important physiological roles. Because of the dense and specific colonization in the midgut crypts ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The symbiotic bacteria are maternally transmitted to the offspring by one of the following mechanisms: superficial bacterial contamination of eggs (egg smearing), probing of the mother's excrement (proctophagy), or deposition of bacterium-containing capsules with eggs (capsule transmission) (6). In several pentatomomorphan bugs, experimental deprivation of the symbiont resulted in retarded growth and/or nymphal mortality (1,13,17,22,24), suggesting that the symbionts may play important roles in the host bugs.…”

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confidence: 99%

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Gut Symbiotic Bacteria of the Genus Burkholderia in the Broad-Headed Bugs Riptortus clavatus and Leptocorisa chinensis (Heteroptera: Alydidae)

Kikuchi

Meng

Fukatsu

2005

Appl Environ Microbiol

182

204

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The Japanese common broad-headed bugs Riptortus clavatus and Leptocorisa chinensis possess a number of crypts in the posterior region of the midgut, whose lumen contains a copious amount of bacterial cells. We characterized the gut symbiotic bacteria by using molecular phylogenetic analysis, light and electron microscopy, in situ hybridization, and PCR-based detection techniques. Restriction fragment length polymorphism analysis of 16S rRNA gene clones suggested that a single bacterium dominated the microbiota in the crypts of the both bug species. The predominant 16S rRNA gene sequences obtained from different individuals and species of the bugs were not identical but were very similar to each other. Homology searches in the DNA databases revealed that the sequences showed the highest levels of similarity (96% to 99%) to the sequences of Burkholderia spp. belonging to the ␤ subdivision of the class Proteobacteria. In situ hybridization with specific oligonucleotide probes confirmed the localization of the Burkholderia symbiont in the lumen of the midgut crypts. Electron microscopy showed that the lumen of the crypts was filled with rod-shaped bacteria of a single morphotype. Molecular phylogenetic analysis demonstrated that the Burkholderia symbionts of the bugs formed a well-defined monophyletic group, although the group also contained several environmental Burkholderia strains. The phylogenetic relationship of the Burkholderia symbionts did not reflect the relationship of the host bug species at all. The sequences from R. clavatus and the sequences from L. chinensis did not form clades but were intermingled in the phylogeny, suggesting that horizontal transmission of the symbiont might have occasionally occurred between populations and species of the bugs.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Gut Symbiotic Bacteria of the Genus Burkholderia in the Broad-Headed Bugs Riptortus clavatus and Leptocorisa chinensis (Heteroptera: Alydidae)

Kikuchi

Meng

Fukatsu

2005

Appl Environ Microbiol

182

204

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“…1D), their lumen filled with a number of specific bacterial symbionts (22,33,44,64). In some stinkbug species, elimination of the symbiont causes retarded growth and nymphal mortality of the hosts (1,38,48,53,54,65,66,82,93,105), indicating pivotal roles of the symbionts for the host insects. Probably because of their extracellular associations, almost all stinkbugs have evolved postnatal mechanisms for symbiont transmission (see below), unlike the intracellular symbiosis established through prenatal transmission (e.g.…”

Section: Exracellular Symbiosis or Gut Symbiosismentioning

confidence: 99%

Endosymbiotic Bacteria in Insects: Their Diversity and Culturability

2009

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Many animals and plants possess symbiotic microorganisms inside their body, wherein intimate interactions occur between the partners. The Insecta, often rated as the most diverse animal group, show various types of endosymbiotic associations, ranging from obligate mutualism to facultative parasitism. Although technological advancements in culture-independent molecular techniques, such as quantitative PCR, molecular phylogeny and in situ hybridization, as well as genomic and metagenomic analyses, have allowed us to directly observe endosymbiotic associations in vivo, the molecular mechanisms underlying insect-microbe interactions are not well understood, because most of these insect endosymbionts are neither culturable nor genetically manipulatable. However, recent studies have succeeded in the isolation of several facultative symbionts by using insect cell lines or axenic media, revolutionizing studies of insect endosymbiosis. This article reviews the amazing diversity of bacterial endosymbiosis in insects, focusing on several model systems with culturable endosymbionts, which provide a new perspective towards understanding how intimate symbiotic associations may have evolved and how they are maintained within insects.

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“…In the families Pentatomidae, Plataspidae, Acanthosomatidae, Scutelleridae and Parastrachiidae, their gut symbionts belong to distinct lineages in the g-Proteobacteia, indicating multiple evolutionary origins of the symbiotic associations (Fukatsu and Hosokawa, 2002;Hosokawa et al, 2006Hosokawa et al, , 2010Prado et al, 2006;Kikuchi et al, 2009;Prado and Almeida, 2009a, b;Kaiwa et al, 2010). In several species of the families Pentatomidae, Plataspidae, Acanthosomatidae, Parastrachiidae and Cydnidae, symbiont-free insects suffered retarded growth and/or nymphal mortality, suggesting mutualistic nature of the symbiotic associations (Mü ller, 1956;Huber-Schneider, 1957;Schorr, 1957;Abe et al, 1995;Fukatsu and Hosokawa, 2002;Hosokawa et al, 2006;Kashima et al, 2006;Kikuchi et al, 2009;Prado and Almeida, 2009b).…”

Section: Introductionmentioning

confidence: 99%

An ancient but promiscuous host–symbiont association between Burkholderia gut symbionts and their heteropteran hosts

2010

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Here, we investigated 124 stinkbug species representing 20 families and 5 superfamilies for their Burkholderia gut symbionts, of which 39 species representing 6 families of the superfamilies Lygaeoidea and Coreoidea were Burkholderia-positive. Diagnostic PCR surveys revealed high frequencies of Burkholderia infection in natural populations of the stinkbugs, and substantial absence of vertical transmission of Burkholderia infection to their eggs. In situ hybridization confirmed localization of the Burkholderia in their midgut crypts. In the lygaeoid and coreoid stinkbugs, development of midgut crypts in their alimentary tract was coincident with the Burkholderia infection, suggesting that the specialized morphological configuration is pivotal for establishment and maintenance of the symbiotic association. The Burkholderia symbionts were easily isolated as pure culture on standard microbiological media, indicating the ability of the gut symbionts to survive outside the host insects. Molecular phylogenetic analysis showed that the gut symbionts of the lygaeoid and coreoid stinkbugs belong to a b-proteobacterial clade together with Burkholderia isolates from soil environments and Burkholderia species that induce plant galls. On the phylogeny, the stinkbug-associated, environmental and gall-forming Burkholderia strains did not form coherent groups, indicating host-symbiont promiscuity among these stinkbugs. Symbiont culturing revealed that slightly different Burkholderia genotypes often coexist in the same insects, which is also suggestive of host-symbiont promiscuity. All these results strongly suggest an ancient but promiscuous host-symbiont relationship between the lygaeoid/coreoid stinkbugs and the Burkholderia gut symbionts. Possible mechanisms as to how the environmentally transmitted promiscuous symbiotic association has been stably maintained in the evolutionary course are discussed.

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Experimentelle studien an der symbiose von Coptosoma scutellatum Geoffr. (Hem. heteropt.)

Cited by 31 publications

References 6 publications

Gut Symbiotic Bacteria of the Genus Burkholderia in the Broad-Headed Bugs Riptortus clavatus and Leptocorisa chinensis (Heteroptera: Alydidae)

Gut Symbiotic Bacteria of the Genus Burkholderia in the Broad-Headed Bugs Riptortus clavatus and Leptocorisa chinensis (Heteroptera: Alydidae)

Endosymbiotic Bacteria in Insects: Their Diversity and Culturability

An ancient but promiscuous host–symbiont association between Burkholderia gut symbionts and their heteropteran hosts

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