Experimentelle Untersuchungen zur Syncarcinogenese

Schm�hl, D.; Thomas, C.; Sattler, W.; Scheld, G.

doi:10.1007/bf00525347

Cited by 34 publications

(2 citation statements)

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“…Porta & Gomez- Dumm (1966) have reported a similar relationship between the development of liver lesions and growth rate with rats receiving ethanol. Schmahl, Thomas, Sattler & Scheld (1965) found that when extremely large quantities of a commercial brandy containing 38 % ethanol were administered to rats, growth was strongly depressed, but neither fibrosis nor other lesions of the liver were observed.…”

Section: Vol 21mentioning

confidence: 97%

Repeated alcoholic intoxication compared with continued consumption of dilute ethanol in experiments with rats on a marginal diet

et al. 1967

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I. The effects have been compared of long-term administration of similar amounts of ethanol given either diluted in drinking water or spaced out in the form of intoxicating doses.2. The effect of ethanol on the development of liver lesions produced by a marginal diet was examined. The measurements of growth rate, water balance, plasma glucose and electrolytes, rate of ethanol elimination, and some behavioural tests for the detection of tolerance and possible signs of neurological disturbance were also included.3. Isocalorically pair-fed growing male rzts were subjected to three different regimens for 6 months: (A) ethanol solution as the sole drinking fluid with z days' intake adjusted to approximately j mg/g body-weight ; ( 8 ) a similar quantity of ethanol given as a single intoxicating dose per 0s on alternate days; (C) oleic acid isocaloric with the ethanol per 0s on alternate days. 4. All the regimens caused fatty liver. Hepatic fibrosis, as judged macro-and microscopically, was equally severe after treatments A and C , but milder after treatment B.j. Growth was retarded by intoxication, which also brought about a large increase in water consumption, without exerting other clear effects on water balance or on plasma electrolytes.The rate of ethanol elimination was increased by repeated intoxication. Behavioural tolerance of the effects of ethanol was also found in the animals subjected to intoxication.Nutritional and pharmacological factors both produce effects on the organism after long use of alcoholic beverages. The amount of ethanol consumed determines its role in the nutritional balance, whereas the temporal distribution of the intake is decisive for the intensity of the pharmacological effects. Improvement in the differentiation between these two aspects of the action of ethanol would serve to advance our understanding of how the hazards involved in the use of alcoholic beverages could be mitigated. Notwithstanding the importance of these considerations, little systematic exploration of the respective roles of the pharmacological and the nutritional effects of ethanol has been made. In long-term experiments with animals, the most common technique has been to provide ethanol solution as the sole source of fluid. Reports have been published also on various effects of intoxicating doses repeated over prolonged periods. There does not seem, however, to be any report of studies in which other conditions have been kept constant, and only the method of administration of a given quantity of ethanol has been varied. The purpose of the present study was to examine this approach, and find criteria for further investigations along similar lines. A further aim was to simulate conditions of a regular but relatively moderate drinking pattern, rather than heavy drinking. A brief preliminary report has been published (Wallgren & Suomalainen, 1966 E X P E R I M E N T A L Criteria of ethanol effectsAs the study was exploratory, broad criteria were chosen. Fatty infiltration and fibrosis of the liver objectively show ef...

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Section: Vol 21mentioning

confidence: 97%

Repeated alcoholic intoxication compared with continued consumption of dilute ethanol in experiments with rats on a marginal diet

et al. 1967

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“…Pese a todo, sabemos que la carcinogénesis por DENA se acelera si junto con este compuesto se administran a las ratas dosis altas de tetracloruro de carbono 64 u otro cancerígeno, el 4-dimetilaminoestilbeno 62 ; en cambio, no parece afectarse irradiando simultáneamente al animal con una dosis total de hasta 320 r, o tras la ingesta prolongada -con vistas a producir una cirrosis-de un licor comercial 64 • 65 • Aun cuando ha sido ampliamente utilizada la hepatectomía como medio de obtener tejido regenerante, con vistas a estudios metabólicos, pocos investigadores se han planteado el problema de en qué modo este proceso de regeneración puede afectar al mecanismo de carcinogénesis y los resultados son, en general, confusos 57 • Las primeras experiencias de Glinos y col. 21 sobre el efecto de la hepatectomía en la carcinogénesis, por el dimetilaminoa-zobenceno (DAB), administrado durante períodos de tiempo que oscilan entre los 30 y 35 días, y extirpando el hígado siempre al final de este período, demostraban una cierta aceleración del proceso carcinogenético en el tiempo, ya que no en el número, puesto que, casi todos los animales, bien hepatectomizados o no, desarrollaban hepatomas dentro de un año después de la administración del compuesto. Este trabajo no concuerda con el de Novikoff y col 5 0 quienes, tras un período similar de cancerización, con el mismo compuesto y con un derivado, el 3metil DAB, no encuentran que la hepatectomía produzca variaciones en la cancerización de sus ratas.…”

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Efecto de la hepatectomia parcial sobre la carcinogénesis hepática producida por la dietilnitrosamina (DENA) en ratas

Morentin

Hernández

2017

revista-de-medicina

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Se estudia el efecto de una hepatectomía parcial (60-70 %), realizada en dos momentos distintos sobre la carcinogénesis hepática por la dietilnitrosamina, en ratas. Para ello se utilizaron 72 ratas macho de raza Wistar, y de un mes de edad al comienzo de la experiencia; 20 de ellas sufrieron una administración del cancerígeno, por vía oral, 6 veces por semana, a una dosis de 5 mgr por kilogramo de peso; los otros animales sufrieron, además, una extirpación hepática, 22 en el primer momento de la carcinogénesis, y los otros 30, tras 15 semanas de administración de DENA. A la muerte de los animales se realizó un estudio anatomopatológico. Los principales resultados, fueron la aparición en los animales hepatectomizados de un nuevo tipo de tumor derivado de las células endoteliales, el llamado hemangioendotelioma. El período de muerte, en cambio, fue muy semejante para todos los animales y asimismo el patrón de afectación lobular, por lo que cabe admitir que el proceso de carcinogénesis por este compuesto sea de tipo cummulativo e irreversible ya que el hepatocito apenas si modifica su carácter tras una fuerte onda regenerativa.

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Enhancement by ethyl alcohol of experimental hepatocarcinogenesis induced byN-nitrosomorpholine

et al. 1997

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The effects of ethyl alcohol (EtOH) during or after treatment with N-nitrosomorpholine (NNM) on hepatocarcinogen-esis, ornithine decarboxylase (ODC) activity and the labeling index of the liver were investigated in male Sprague-Dawley rats. Rats were given drinking water containing NNM for 8 weeks and received i.p. injections of 1 g EtOH/kg body weight every other day during or after treatment with NNM. Pre-neoplastic and neoplastic lesions staining positively for gluta-thione-S-transferase, placental type (GST-P), were examined immunohistochemically. At the end of experiment at week 16, administration of EtOH after NNM treatment had no significant effect on the number and size of GST-P-positive hepatic lesions, whereas administration of EtOH during NNM treatment significantly increased the number and percentage area but not the mean area of GST-P-positive hepatic lesions. EtOH caused significant increases in the ODC activity of the liver and in the labeling indices of enzyme-altered lesions and the adjacent hepatocytes after the cessation of EtOH administration but not during EtOH treatment. Our findings indicate that EtOH enhances hepatocarcinogenesis and suggest that this effect may be closely related to the increases in ODC activity and cell proliferation in enzyme-altered lesions and the adjacent liver after EtOH treatment. Int. Consumption of alcoholic beverages has been linked to an increased risk of hepatocellular carcinoma (HCC) in humans. Case-control studies (Oshima et al., 1984; Tsukuma et al., 1990) of HCC and alcohol consumption have shown a significantly increased risk of HCC in heavy drinkers. Ethanol (EtOH) also has been found to enhance hepatic tumorigenesis (Yamagiwa et al., 1994). However, the mechanism by which alcohol induces cancers in humans is not clear. Epidemiologically, Tsukuma et al. (1993) have reported that the risk of HCC is significantly increased in former heavy drinkers but is small and insignificant in current heavy drinkers. These findings suggest that alcohol at an initiation phase may have an important role in hepatocarcinogenesis. Therefore, to investigate this possibility , in the present work, we examined the effect of EtOH given during or after administration of the carcinogen N-nitrosomorpho-line (NNM) on hepatocarcinogenesis in rats. MATERIAL AND METHODS Animals Ninety inbred Sprague-Dawley rats, initially weighing 150 g, were purchased from Japan SLC (Shizuoka, Japan). Rats were housed in suspended cages with wire mesh bottoms in a room with controlled temperature (21 6 1°C) and humidity (40 6 10%) and a 12:12 light:darkness cycle and given free access to regular chow pellets (Nihon-Nosan, Yokohama, Japan). Experimental design Animals were randomly divided into 3 groups of 30 rats each and from the beginning of the experiment were given drinking water containing 175 mg/l of NNM (Sigma, St. Louis, MO) for 8 weeks. NNM was dissolved in distilled water at a concentration of 50 g/l and stored in a cool place. The stock solution was diluted to 175 mg/l with tap water just...

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Experimentelle Untersuchungen zur Syncarcinogenese

Cited by 34 publications

References 6 publications

Repeated alcoholic intoxication compared with continued consumption of dilute ethanol in experiments with rats on a marginal diet

Repeated alcoholic intoxication compared with continued consumption of dilute ethanol in experiments with rats on a marginal diet

Efecto de la hepatectomia parcial sobre la carcinogénesis hepática producida por la dietilnitrosamina (DENA) en ratas

Enhancement by ethyl alcohol of experimental hepatocarcinogenesis induced byN-nitrosomorpholine

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