Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Kumaran, Malina; Kalaichelv, P.T.; Santhi, R.

doi:10.3923/mj.2015.36.42

Cited by 7 publications

(6 citation statements)

References 19 publications

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“…Las celulasas producidas en ambos medios de cultivo presentaron mayores AE cuando la reacción se realizó a pH 7,0 (Figuras 1 y 2), evidenciándose que presentan una mejor actividad que a pH 5,0. Estos resultados coinciden con los publicados para las celulasas producidas por B. licheniformis MTCC429, a partir de residuos agroindustriales (Bala Kumaran et al 2015), por las obtenidas por Pseudomonas sp. y Serratia sp., aisladas de residuos de salvado de trigo (Prabesh et al 2016) y para la producida a partir de Acinetobacter junii GAC 16.2 (Banerjee et al 2020).…”

Section: Resultados Y Discusiónunclassified

“…Los medios A1 y A2 permiten la producción de celulasas a partir de la cepa C6M2 obteniendo actividades de 30,19 ± 0,61 U/mL y 30,74 ± 0,95 U/mL, que son superiores a las producidas en otros sustratos formulados con residuos agroindustriales. Es así, como las celulasas producidas por B. licheniformis 2D55, en un medio preparado con cáscaras de arroz y bagazo de caña, obtuvieron una actividad CMCasa 0,38 U/mL (Kazeem et al 2017) y otro estudio reportó 0,50 U/mL, para celulasas obtenidas en un caldo CMC con un hidrolizado de bagazo de caña al 5 %, a partir de B. licheniformis MTCC 429 (Bala Kumaran et al 2015).…”

Section: Resultados Y Discusiónunclassified

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Obtención de celulasas bacterianas usando residuos orgánicos generados en plazas de mercado

Sánchez-Castelblanco,

Heredia-Martín

2024

Rev. U.D.C.A Act. & Div. Cient.

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Los residuos agrícolas son una fuente de celulosa que puede ser aprovechada para producir enzimas hidrolíticas, como las celulasas, mediante acción microbiana. Estas celulasas son utilizadas en procesos extractivos de biomoléculas, en la producción de biogás, en la industria textil, detergente, alimentaria y del papel. El propósito del estudio es la obtención de celulasas bacterianas utilizando residuos orgánicos, generados en plazas de mercado. Se realizaron dos medios de cultivo (A1 y A2), a partir de ameros de mazorca y cáscaras de leguminosas, ajustando el pH del medio A1 a 7,0 y el de A2 a 5,0. Los medios fueron fermentados por 75 horas, mediante la cepa bacteriana C6M2, aislada de residuos de plazas de mercado, monitoreando la actividad enzimática, la concentración de azúcares reductores y la celulosa residual. La máxima actividad celulolítica se logró a las 56 horas de fermentación en A1 y a las 32 horas, en A2. El extracto enzimático se precipitó, dializó y ultrafiltró, obteniendo una actividad final de 9,07 ± 0,48 U/mL. Los ameros y las cáscaras de leguminosa se pueden aprovechar como sustratos en la producción de celulasas, con posibles aplicaciones en procesos donde requieran bajos grados de pureza.

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Section: Resultados Y Discusiónunclassified

Obtención de celulasas bacterianas usando residuos orgánicos generados en plazas de mercado

Sánchez-Castelblanco,

Heredia-Martín

2024

Rev. U.D.C.A Act. & Div. Cient.

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“…Ibrahim et al [52] in their study found a nearly similar optimum temperature of 37°C for maximum cellulase activity but the incubation time (24 h) for the same was less than the present finding for the bacterium P. aeruginosa . In the study of Kumaran et al [53], optimum pH of 7.0, the temperature of 35°C and an incubation time of 48 h were determined at which the bacterium Bacillus licheniformis MTCC 429 achieve both the maximum cellulase production and activity. The optimum values of pH, temperature, and incubation time for maximum cellulase yield obtained from Kumaran et al [53] found to be more closely to the optimum values found for those same process parameters (pH 6.75, temperatures: 37.5°C and incubation time: 42 h) in the current investigation.…”

Section: Discussionmentioning

confidence: 99%

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

2023

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Among 24 isolated cellulolytic bacteria from Similipal Biosphere Reserve, the most efficient isolate was recognized as a strain of Bacillus albus. This strain of B. albus was evaluated for cellulase production and the cellulase activity was measured in submerged fermentation using substrate carboxymethyl cellulose (CMC). Different nutritional (carbon, nitrogen, and metal‐ion sources) and physical variables (pH, temperature, substrate concentration, and incubation time) during the growth of B. albus were optimized to obtain maximum cellulase activity. The highest cellulase activity of 5.79 U/mL for B. albus was observed at pH 6.75, temperature 37.5°C, CMC concentration 8.5 g/L, and 42 h incubation time. Further, supplementation of glucose as a subsidiary carbon source, yeast extract, peptone as nitrogen sources, and MgSO4 and MnSO4 as metal‐ion sources enhance the cellulase activity of B. albus. The purified enzyme was reported to have a molecular weight of ∼54 kDa as determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. A zymogram analysis evidenced the cellulase activity of the purified enzyme fractions obtained from diethylaminoethyl cellulose chromatography. The purified cellulase was reported to have an optimum pH and temperature of 7.0°C and 50°C, respectively with a capacity of retaining its 60% residual activity within pH 6.0–8.0 and temperature 30–40°C, respectively. The metal ions, K+ and Na+ were the activators, while Pb2+ and Hg2+ were the inhibitors for the purified cellulase. The purified cellulase showed Km and Vmax values of 0.38 M and 8.19 U/mL, respectively, in presence of the substrate CMC and also simultaneous consumption of both hexose and pentose sugars.

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“…Bacterial cellulases have gained more attention than their fungal counterparts for several reasons. Bacterial cellulases are preferred due to their lower production costs compared to fungal cellulases [ 4 , 5 ]. Moreover, bacteria have faster growth rates than fungi, enabling them to reach high cell densities more quickly.…”

Section: Introductionmentioning

confidence: 99%

Optimization, gene cloning, expression, and molecular docking insights for enhanced cellulase enzyme production by Bacillus amyloliquefaciens strain elh1

El-Khamisi,

Soliman,

El-Sayed

et al. 2024

Microb Cell Fact

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Background In this study, we isolated a cellulase-producing bacterium, Bacillus amyloliquefaciens strain elh, from rice peel. We employed two optimization methods to enhance the yield of cellulase. Firstly, we utilized a one-variable-at-a-time (OVAT) approach to evaluate the impact of individual physical and chemical parameters. Subsequently, we employed response surface methodology (RSM) to investigate the interactions among these factors. We heterologously expressed the cellulase encoding gene using a cloning vectorin E. coli DH5α. Moreover, we conducted in silico molecular docking analysis to analyze the interaction between cellulase and carboxymethyl cellulose as a substrate. Results The bacterial isolate eh1 exhibited an initial cellulase activity of 0.141 ± 0.077 U/ml when cultured in a specific medium, namely Basic Liquid Media (BLM), with rice peel as a substrate. This strain was identified as Bacillus amyloliquefaciens strain elh1 through 16S rRNA sequencing, assigned the accession number OR920278 in GenBank. The optimal incubation time was found to be 72 h of fermentation. Urea was identified as the most suitable nitrogen source, and dextrose as the optimal sugar, resulting in a production increase to 5.04 ± 0.120 U/ml. The peak activity of cellulase reached 14.04 ± 0.42 U/ml utilizing statistical optimization using Response Surface Methodology (RSM). This process comprised an initial screening utilizing the Plackett–Burman design and further refinement employing the BOX -Behnken Design. The gene responsible for cellulase production, egl, was effectively cloned and expressed in E. coli DH5α. The transformed cells exhibited a cellulase activity of 22.3 ± 0.24 U/ml. The egl gene sequence was deposited in GenBank with the accession number PP194445. In silico molecular docking revealed that the two hydroxyl groups of carboxymethyl cellulose bind to the residues of Glu169 inside the binding pocket of the CMCase. This interaction forms two hydrogen bonds, with an affinity score of −5.71. Conclusions Optimization of cultural conditions significantly enhances the yield of cellulase enzyme when compared to unoptimized culturing conditions. Additionally, heterologous expression of egl gene showed that the recombinant form of the cellulase is active and that a valid expression system can contribute to a better yield of the enzyme.

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Exploitation of Agro-Industrial Wastes as Substrates for Cellulase Production by Bacillus licheniformis MTCC 429

Cited by 7 publications

References 19 publications

Obtención de celulasas bacterianas usando residuos orgánicos generados en plazas de mercado

Obtención de celulasas bacterianas usando residuos orgánicos generados en plazas de mercado

Purified cellulase‐mediated simultaneous sugar utilization by Bacillus albus isolated from Similipal, Odisha, India

Optimization, gene cloning, expression, and molecular docking insights for enhanced cellulase enzyme production by Bacillus amyloliquefaciens strain elh1

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