Exploitation of microbes for enhancing bacoside content and reduction of Meloidogyne incognita infestation in Bacopa monnieri L

Abstract

Despite the vast exploration of rhizospheric microbial wealth for crop yield enhancement, knowledge about the efficacy of microbial agents as biocontrol weapons against root-knot disease is scarce, especially in medicinal plants, viz., Bacopa monnieri. In the present investigation, rhizospheric microbes, viz., Bacillus megaterium, Glomus intraradices, Trichoderma harzianum ThU, and their combinations were evaluated for the management of Meloidogyne incognita (Kofoid and White) Chitwood and bacoside content enhancement in B. monnieri var CIM-Jagriti. A novel validated method Fourier transform near infrared was used for rapid estimation of total bacoside content. A significant reduction (2.75-fold) in root-knot indices was observed in the combined treatment of B. megaterium and T. harzianum ThU in comparison to untreated control plants. The same treatment also showed significant enhancement (1.40-fold) in total bacoside contents (plant active molecule) content using Fourier transform near-infrared (FT-NIR) method that analyses samples rapidly in an hour without solvent usage and provides ample scope for natural product studies.

Microbe-based technology ameliorates glandular trichomes, secondary metabolites and antioxidants inPelargonium graveolensL'Hér.

“…In the case of G. intraradices the inoculum was propagated on maize ( Zea mays ) roots for 10 weeks in a 1:1 (v/v) mixture of sterilized sand and soil (5 kg) with a low phosphorus content (7.5 kg ha −1 ) and subsequently left to shade‐dry for 2 weeks. The inoculum potential of G. intraradices used in the present experiment consisted of soil containing spores (8–10 spores g −1 ) with colonized roots of maize …”

“…Therefore an efficient and ecofriendly alternative method for increased production is needed. In this context, the utilization of organic materials and bioinoculants to increase various crop yields is well documented . However, no specific study dealing with the effect of bioinoculants on the growth, essential oil components, total antioxidant content and glandular trichomes of P. graveolens has been done, especially in the context of synergistic application.…”

Microbial secondary metabolites ameliorate growth, in planta contents and lignification in Withania somnifera (L.) Dunal

“…The maximum increase in withaferin A by 4.00 fold was recorded in BS_CC followed by 3.00 fold in both STR_CF and TH_CC treatments as compared to the control plants (Table 1). Recently, a study showed that application of plant growth promoting microbes significantly enhanced secondary metabolite content in medicinal and aromatic plants like Bacopa monnieri, W. somnifera and Pelargonium graveolens (Rajasekar and Elango 2011;Gupta et al 2015bGupta et al , 2016. Increase in antioxidant capacity in TH_CF could also be positively correlated with increased Withanolide A, a well known antioxidant molecule.…”

“…MTN14 (KF699062) and T. harzianum ThU were individually maintained on Luria-Bertani (LB) agar, glucose yeast malt (GYM) agar, and potato dextrose agar (PDA) respectively at room temperature and subcultured monthly. The selection of cultures was done on the basis of their plant growth enhancing traits (Gupta et al 2015b;Gupta and Pandey 2015).…”

Development of microbial consortia for the management of leaf blight disease of coconut

“…L effectively and even performed better than chemical nematicides as they colonize root and have direct interaction making impact on plant health and nematode management (Gupta et al, 2014).…”