Exploitation of phosphorescent labelling reagent of fullerol-fluorescein isothiocyanate and new method for the determination of trace alkaline phosphatase as well as forecast of human diseases

Fluorescein Hydrazones as Novel Nonintercalative Topoisomerase Catalytic Inhibitors with Low DNA Toxicity

“…Fluorescein (1) has been derivatized and/or functionalized particularly on the top ring with an isothiocyanate (−NC S) group named fluorescein isothiocyanate (FITC, Figure 2). FITC has been widely utilized as diverse forms to understand various physicochemical, photophysical, and biological implications; FITC-labeled PEO−PCL−PEO triblock copolymer, 12 FITC−deacetylated chitin conjugates, 13 FITC-labeled human plasma, 14 FITC-labeled collagen, 15 FITC−PEO conjugate, 16 FITC−cyclodextrin conjugates, 17 FITC−fullerol conjugates, 18 insulin−fluorescein conjugates, 19 bifunctional polyacrylamides containing vancomycin and fluorescein, 20 a fluorescein− ruthenium−octa-arginine conjugate, 21 lithocholyl−lysyl−fluorescein conjugates, 22 dimethylamionapthamide−fluorescein conjugates, 23 FITC-labeled lectin, 24 FITC labeling at the 5′end of DNA with intercalating compound conjugates, 25 and FITC−dextran conjugates. 26 Fluorescein also functionalized on the top ring with a carboxylic acid group fluorescein carboxylic acid (FCA), which has been used as precursor for the synthesis of oligonucleotides (e.g., fluorescein phosphoramidites, FAM), 27 fluorescein-tagged peptoid conjugates, 28,29 characterizing RecA−DNA interaction, 30 fluorescein-C-and O-glycosides, 31 fluorescein labeled 7methylguanosinemonophosphate, 32 and ifenprodil conjugates.…”

“…Fluorescein ( 1 ) has been derivatized and/or functionalized particularly on the top ring with an isothiocyanate (−NCS) group named fluorescein isothiocyanate (FITC, Figure ). FITC has been widely utilized as diverse forms to understand various physicochemical, photophysical, and biological implications; FITC-labeled PEO–PCL–PEO triblock copolymer, FITC–deacetylated chitin conjugates, FITC-labeled human plasma, FITC-labeled collagen, FITC–PEO conjugate, FITC–cyclodextrin conjugates, FITC–fullerol conjugates, insulin–fluorescein conjugates, bifunctional polyacrylamides containing vancomycin and fluorescein, a fluorescein–ruthenium–octa-arginine conjugate, lithocholyl–lysyl–fluorescein conjugates, dimethylamionapthamide–fluorescein conjugates, FITC-labeled lectin, FITC labeling at the 5′-end of DNA with intercalating compound conjugates, and FITC–dextran conjugates …”

A Novel Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Cytochrome C and Forecast of Human Diseases

“…As a result, F-ol could emit strong and steady RTP. Then the reaction was carried out between HOOC-in Cyt C molecule and -OH in F-ol molecule [18], then a nonphosphorescence compound F-olCyt C formed (Scheme 3) causing the RTP of F-ol quenching sharply (Figure 1, curve 5.5 � ).…”

“…Therefore, searching for a high sensitive and accurate method for the determination of Cyt C in biological samples and discussing the relativity between the content of Cyt C and cell apoptosis have become research focus with high academic value and great significance. There have been many reports on the synthesis of multihydroxyl C 60 derivatives, F-ol derivatives, aminophenol derivatives, dendritic fullerene derivatives [13][14][15], the fluorescent property of water-soluble F-ol [16], and the determination of alkaline phosphatase [17,18], glucose [19], As (V) [20], and Mn 2+ [21] based on phosphorescent property of F-ol, showing broad prospects of F-ol in analytical application.…”