Exploitation of polyploids from 39 citrus seedling populations

Guo, Wen‐Wu; Liang, Wangwang; Xie, Kai‐Dong; Xia, Qiang‐Ming; Fu, Jin; Guo, Dayong; Xie, Zongzhou; Wu, Xiaomeng; Xu, Qiang; Yi, Hualin; Deng, Xiaodong

doi:10.17660/actahortic.2016.1135.2

Cited by 13 publications

(3 citation statements)

References 6 publications

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“…Nuclei were isolated from young leaves and 15-day-old seeds of ‘XJ2’, and run through a flow-cytometer (PARTEC, Germany) after they were stained with DAPI solution (PARTEC, HR-B) following a protocol reported by Guo et al [ 54 ]. Three replicates were carried out in this part.…”

Section: Methodsmentioning

confidence: 99%

Reduced expression of a subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1βγ, confers flat fruit abortion in peach by regulating sugar and starch metabolism

et al. 2021

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Background Fruit abortion is a major limiting factor for fruit production. In flat peach, fruit abortion is present in the whole tree of some accessions during early fruit development. However, the physiological factors and genetic mechanism underlying flat fruit abortion remain largely elusive. Results In this study, we have revealed that the fertilization process was accomplished and the reduction of sucrose and starch contents might result in flat fruit abortion. By combining association and gene expression analysis, a key candidate gene, PpSnRK1βγ, was identified. A 1.67-Mb inversion co-segregated with flat fruit shape altered the promoter activity of PpSnRK1βγ, resulting in much lower expression in aborting flat peach. Ectopic transformation in tomato and transient overexpression in peach fruit have shown that PpSnRK1βγ could increase sugar and starch contents. Comparative transcriptome analysis further confirmed that PpSnRK1βγ participated in carbohydrate metabolism. Subcellular localization found that PpSnRK1βγ was located in nucleus. Conclusions This study provides a possible reason for flat fruit abortion and identified a critical candidate gene, PpSnRK1βγ, that might be responsible for flat fruit abortion in peach. The results will provide great help in peach breeding and facilitate gene identification for fruit abortion in other plant species.

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Section: Methodsmentioning

confidence: 99%

Reduced expression of a subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1βγ, confers flat fruit abortion in peach by regulating sugar and starch metabolism

et al. 2021

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“…Nuclei were isolated from young leaves and 15-day-old seeds of 'XJ2', and run through a ow-cytometer (PARTEC, Germany) after they were stained with DAPI solution (PARTEC, HR-B) following a protocol reported by Guo et al [54]. Three replicates were carried out in this part.…”

Section: Ploidy Detection Using Ow Cytometrymentioning

confidence: 99%

A subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1γ, confers flat fruit abortion in peach by regulating sugar and starch metabolism

Guo

Cao

Yao

et al. 2020

Preprint

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Background: Fruit abortion is a major limiting factor for fruit production. In flat peach, fruit abortion is present in the whole tree of some accessions during early fruit development. However, the physiological factors and genetic mechanism underlying flat fruit abortion remain largely elusive. Results: In this study, we have revealed that the fertilization process was accomplished and the reduction of sucrose and starch contents might result in flat fruit abortion. By combining association and gene expression analysis, a key candidate gene, PpSnRK1γ, was identified. A 1.67-Mb inversion co-segregated with flat fruit shape altered the promoter activity of PpSnRK1γ, resulting in much lower expression in aborting flat peach. Ectopic transformation in tomato and transient overexpression in peach fruit have shown that PpSnRK1γ could increase sugar and starch contents. Comparative transcriptome analysis further confirmed that PpSnRK1γ participated in carbohydrate metabolism. Subcellular localization found that PpSnRK1γ was located in nucleus. Conclusions: This study clarified the reason for flat fruit abortion and identified a critical candidate gene, PpSnRK1γ, responsible for fruit abortion in peach. The results would provide great help in peach breeding and facilitate gene identification for fruit abortion in other plant species.

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“…Polyploid orchids have high commercial value because of their larger flowers, intensified coloration, thicker stems and leaves, enhanced fertility, and better adaptability (Bolaños-Villegas and Chen 2022; Vilcherrez-Atoche et al 2022). Natural polyploids usually originate from the production of unreduced (2n) gametes because of failure during meiosis accompanied by self-pollination (Guo et al 2016;Krahulcov a and Krahulec, 2000;Sattler et al 2016;Talluri 2011). Progress rarely occurs with meiotic failure and successful seed germination (Bory et al 2008).…”

mentioning

confidence: 99%

In Vitro Induction of Polyploidy by Colchicine in the Protocorm of the Orchid Dendrobium wardianum Warner

Wang,

Zhuo,

Arslan

et al. 2023

horts

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Dendrobium wardianum is a key ornamental plant and a valuable traditional Chinese medicine. This research aimed to find the optimal protocol for in vitro inducement of polyploidy in D. wardianum by treating protocorms with colchicine (an antimitotic agent). The experiment consisted of two series of treatments. For the first treatment, the protocorms were subjected to colchicine concentrations of 25, 75, 125, 250, and 500 μM (weight/volume) for 6, 12, and 24 hours. For the second treatment, protocorms were cultivated in culture medium with colchicine (25, 75, 125, and 250 μM) for 30 days. A total of 18 polyploids were confirmed by chromosome counts and anatomical parameters. Polyploids had broad, dark green leaves with increased stem lengths compared with those of diploids. The optimal protocol for these two methods consisted of soaking in 250 μM of colchicine solution for 12 hours, resulting in inductivity of 26%, and cultivating in 75 μM for 30 days, resulting in a mutation rate of 34%. A comparison of these two protocols showed that the latter one induced more stable polyploids, but that the survival rate was slightly lower. The survival and induced mutation rates of these plants were significantly influenced by the colchicine concentration and exposure time. Higher concentrations for longer periods of time resulted in greater mortality rates and longer-lasting side effects. The protocol involving a solid medium and colchicine is worth considering. It will be intriguing to examine this methodology for the induction of stable polyploids of other orchid species.

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Exploitation of polyploids from 39 citrus seedling populations

Cited by 13 publications

References 6 publications

Reduced expression of a subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1βγ, confers flat fruit abortion in peach by regulating sugar and starch metabolism

Reduced expression of a subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1βγ, confers flat fruit abortion in peach by regulating sugar and starch metabolism

A subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1γ, confers flat fruit abortion in peach by regulating sugar and starch metabolism

In Vitro Induction of Polyploidy by Colchicine in the Protocorm of the Orchid Dendrobium wardianum Warner

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