Exploitation of the chick embryo chorioallantoic membrane (CAM) as a platform for anti-metastatic drug testing

Pawlikowska, Patrycja; Tayoun, Tala; Oulhen, Marianne; Faugeroux, Vincent; Rouffiac, Valérie; Aberlenc, Agathe; Pommier, Anne-Laure; Honoré, Aurélie; Marty, Virginie; Bawa, Olivia; Lacroix, Ludovic; Scoazec, Jean‐Yves; Chauchereau, Anne; Laplace‐Builhé, Corinne; Farace, Françoise

doi:10.1038/s41598-020-73632-w

Cited by 49 publications

(49 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Hen's egg test-chorioallantoic membrane (HET-CAM) model is widely used in translational research and has the potential to bridge between conventional in vitro and in vivo methods, and such, to at least reduce the number of laboratory animals required [3][4][5][6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI

Löffler

Hamp

Scheidhauer

et al. 2021

Cancers

View full text Add to dashboard Cite

Assessment of biodistribution and specific tumor accumulation is essential for the development of new radiopharmaceuticals and requires animal experiments. The HET-CAM (hens-egg test—chorioallantoic membrane) model can be used in combination with the non-invasive imaging modalities PET and MRI for pre-selection during radiopharmaceutical development to reduce the number of animal experiments required. Critical to the acceptance of this model is the demonstration of the quantifiability and reproducibility of these data compared to the standard animal model. Tumor accumulation and biodistribution of the PSMA-specific radiotracer [18F]F-siPSMA-14 was analyzed in the chick embryo and in an immunodeficient mouse model. Evaluation was based on MRI and PET data in both models. γ-counter measurements and histopathological analyses complemented these data. PSMA-specific accumulation of [18F]F-siPSMA-14 was successfully demonstrated in the HET-CAM model, similar to the results obtained by mouse model studies. The combination of MR and PET imaging allowed precise quantification of peptide accumulation, initial assessment of biodistribution, and accurate determination of tumor volume. Thus, the use of the HET-CAM model is suitable for the pre-selection of new radiopharmaceuticals and potentially reduces animal testing in line with the 3Rs principles of animal welfare.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI

Löffler

Hamp

Scheidhauer

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…Furthermore, the results do not translate well to humans in clinical trials, explaining in part the failure of hypoxic pro-drugs to make it to market [ 72 ]. The CAM model provides an attractive alternative to mice as it is cost- and time-efficient allowing the assessment of tumor growth and metastasis within 5 to 7 days following inoculation [ 73 , 74 ]. Our finding, that CAM xenografts also successfully recapitulates the tumor hypoxic microenvironment in a various cancer cell lines and in patient-derived xenografts, suggests that the CAM model may provide a relevant preclinical platform to test the efficacy of hypoxia-activated pro-drugs in a time and cost-effective manner.…”

Section: Discussionmentioning

confidence: 99%

The Chicken Chorioallantoic Membrane Tumor Assay as a Relevant In Vivo Model to Study the Impact of Hypoxia on Tumor Progression and Metastasis

Harper

Yatsyna

Charbonneau

et al. 2021

Cancers

View full text Add to dashboard Cite

Hypoxia in the tumor microenvironment is a negative prognostic factor associated with tumor progression and metastasis, and therefore represents an attractive therapeutic target for anti-tumor therapy. To test the effectiveness of novel hypoxia-targeting drugs, appropriate preclinical models that recreate tumor hypoxia are essential. The chicken ChorioAllantoic Membrane (CAM) assay is increasingly used as a rapid cost-effective in vivo drug-testing platform that recapitulates many aspects of human cancers. However, it remains to be determined whether this model recreates the hypoxic microenvironment of solid tumors. To detect hypoxia in the CAM model, the hypoxic marker pimonidazole was injected into the vasculature of tumor-bearing CAM, and hypoxia-dependent gene expression was analyzed. We observed that the CAM model effectively supports the development of hypoxic zones in a variety of human tumor cell line-derived and patient’s tumor fragment-derived xenografts. The treatment of both patient and cell line-derived CAM xenografts with modulators of angiogenesis significantly altered the formation of hypoxic zones within the xenografts. Furthermore, the changes in hypoxia translated into modulated levels of chick liver metastasis as measured by Alu-based assay. These findings demonstrate that the CAM xenograft model is a valuable in vivo platform for studying hypoxia that could facilitate the identification and testing of drugs targeting this tumor microenvironment.

show abstract

“…DMSA-TiO 2 was evaluated in vivo with a chick chorioallantoic membrane (CAM) model for its antiangiogenic activity (Rovithi et al., 2017 ; Vu et al., 2018 ; Pawlikowska et al., 2020 ). Briefly, ethanol was used to cleanse the surface of the fertilized chicken eggs, and the incubator was used to incubate the eggs in a humanized 60% atmosphere at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Synergic fabrication of succimer coated titanium dioxide nanomaterials delivery for in vitro proliferation and in vivo examination on human aortic endothelial cells

Song

et al. 2021

Drug Delivery

View full text Add to dashboard Cite

The probable nanotoxicity to human health and the environment is a significant challenge for the sustainable application of nanomaterials in medicine. The cytototoxical effect of succimer (meso-2,3-dimercaptosuccinic acid-DMSA) coated titanium dioxide (DMSA-TiO 2 ) with cultured human aortic endothelial cells (HAoECs) was assessed in this investigation. Our findings have shown that DMSA-TiO 2 can be accumulated in HAoECs and dispersed in a cytoplasm on the culture medium. DMSA-cytotoxicity TiO 2 effects were dose-responsive, and the concentrations were of little toxicity, and MTT stain testing showed that they had only 0.02 mg ml −1 . Meanwhile, the lactate dehydrogenase biomarker was not considerably more remarkable than the biomarker from untreated (control) cells (free DMSA-TiO 2 ). Though, also without any apparent signs of cell damage, the endocrine functions for prostacyclin I-2 and endothelin-1 and the urea transporter functions were modified. In addition, in vitro endothelial tube development has been shown that HAoECs could induce angiogenesis even with small amounts of DMSA-TiO 2 (0.01 and 0.02 mg ml −1 ). Further, we have examined the in vivo toxicity and biochemical parameter by animal model. Furthermore, in vivo assessments designated that the resulting DMSA-TiO 2 presented synergistic activities of angiogenesis activity. Overall, these findings show the cytotoxicity of DMSA-TiO 2 and could induce adverse effects on normal endothelial cells.

show abstract

Exploitation of the chick embryo chorioallantoic membrane (CAM) as a platform for anti-metastatic drug testing

Cited by 49 publications

References 35 publications

Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI

Comparison of Quantification of Target-Specific Accumulation of [18F]F-siPSMA-14 in the HET-CAM Model and in Mice Using PET/MRI

The Chicken Chorioallantoic Membrane Tumor Assay as a Relevant In Vivo Model to Study the Impact of Hypoxia on Tumor Progression and Metastasis

Synergic fabrication of succimer coated titanium dioxide nanomaterials delivery for in vitro proliferation and in vivo examination on human aortic endothelial cells

Contact Info

Product

Resources

About