Exploiting New Systems-Based Strategies to Elucidate Plant-Bacterial Interactions in the Rhizosphere

Kiely, Patrick; Haynes, Jill M.; Higgins, Chris; Franks, Ashley E.; Mark, G. L.; Morrissey, John P.; O’Gara, Fergal

doi:10.1007/s00248-006-9019-y

Cited by 91 publications

(52 citation statements)

References 68 publications

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“…While these bacteria utilize the nutrients that are released from the host for their growth, they also secrete metabolites into the rhizosphere. Several of these metabolites can act as signalling compounds that are perceived by neighbouring cells within the same micro-colony, by cells of other bacteria that are present in the rhizosphere, or by root cells of the host plant Bais et al 2004;Gray and Smith 2005;Kiely et al 2006).…”

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confidence: 99%

Plant responses to plant growth-promoting rhizobacteria

2007

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Non-pathogenic soilborne microorganisms can promote plant growth, as well as suppress diseases. Plant growth promotion is taken to result from improved nutrient acquisition or hormonal stimulation. Disease suppression can occur through microbial antagonism or induction of resistance in the plant. Several rhizobacterial strains have been shown to act as plant growth-promoting bacteria through both stimulation of growth and induced systemic resistance (ISR), but it is not clear in how far both mechanisms are connected. Induced resistance is manifested as a reduction of the number of diseased plants or in disease severity upon subsequent infection by a pathogen. Such reduced disease susceptibility can be local or systemic, result from developmental or environmental factors and depend on multiple mechanisms. The spectrum of diseases to which PGPRelicited ISR confers enhanced resistance overlaps partly with that of pathogen-induced systemic acquired resistance (SAR). Both ISR and SAR represent a state of enhanced basal resistance of the plant that depends on the signalling compounds jasmonic acid and salicylic acid, respectively, and pathogens are differentially sensitive to the resistances activated by each of these signalling pathways. Root-colonizing Pseudomonas bacteria have been shown to alter plant gene expression in roots and leaves to different extents, indicative of recognition of one or more bacterial determinants by specific plant receptors. Conversely, plants can alter root exudation and secrete compounds that interfere with quorum sensing (QS) regulation in the bacteria. Such two-way signalling resembles the interaction of root-nodulating Rhizobia with legumes and between mycorrhizal fungi and roots of the majority of plant species. Although ISR-eliciting rhizobacteria can induce typical early defence-related responses in cell suspensions, in plants they do not necessarily activate defence-related gene expression. Instead, they appear to act through priming of effective resistance mechanisms, as reflected by earlier and stronger defence reactions once infection occurs.

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confidence: 99%

Plant responses to plant growth-promoting rhizobacteria

2007

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“…Roots and soil bacteria are engaged in highly specific chemical communication, leading to biologically significant interactions (2,17,18). The symbiotic association between the Rhizobiaceae family (including the genera Azorhizobium, Bradyrhizobium, and Sinorhizobium) and leguminous plants constitutes the best known example of this chemical exchange of information.…”

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confidence: 99%

Root-Microbe Communication through Protein Secretion

De‐la‐Peña

Lei

Watson

et al. 2008

Journal of Biological Chemistry

148

151

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Biotic interactions in the rhizosphere are biologically important, and although many of those interactions have been well studied, the role of secreted proteins in the cross-talk between microbes and roots has not been investigated. Here, protein secretion was studied during the communication between the roots of two plants (Medicago sativa and Arabidopsis thaliana) and the bacterial symbiont of one of these species (Sinorhizobium meliloti strain Rm1021) and an opportunistic bacterial pathogen of A. thaliana (Pseudomonas syringae pv. tomato DC3000) using a proteomic approach. It was found that protein exudation in the M. sativa-S. meliloti interaction caused an increase in the secretion of seven plant proteins, such as hydrolases, peptidases, and peroxidases among others in two or more time points compared with the plant control. In addition, four proteins, all of bacterial origin, were increased 1.5-fold more in this interaction compared with S. meliloti alone. However, these proteins were not induced when M. sativa was inoculated with P. syringae DC3000. The interaction between A. thaliana and P. syringae DC3000 highly induced the secretion of several plant proteins related to defense soon after initial contact with P. syringae, but these proteins were not secreted in the incompatible interaction with S. meliloti. The results of this study reveal a specific, protein level cross-talk between roots and microbes. These results suggest that secreted proteins may be a critical component in the process of signaling and recognition that occurs between compatible and incompatible interactions.

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“…In this technique, plants are exposed to 13 CO 2 , which has a heavier carbon atom than regular CO 2 , metabolized by the plant, and deposited in the rhizosphere through rhizodeposition and utilized by the microbes present in the rhizosphere. The nucleic acids of the microbes utilizing the 13 CO 2 will be heavier than the noninteracting microbes and analyzed using density gradient centrifugation (Kiely et al 2006) and also yield the entire genome of all the participating microbes in the rhizosphere (Singh et al 2004). In addition, the recent development of "omics" technologies coupled with bioinformatics studies is appropriate to study the rhizospheric soil microbe's interactions at community levels to species level.…”

Section: Methods For Studying Rhizosphere Interactionsmentioning

confidence: 99%

Plant Root Secretions and Their Interactions with Neighbors

De‐la‐Peña

Badri

Loyola‐Vargas

2011

Signaling and Communication in Plants

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The rhizosphere biology at the molecular level has advanced dramatically since last decade. The continuous supply of carbon compounds from plant roots engages complex interactions among rhizosphere organisms including interactions between microbes and plants and between plants with other plants being these of the same or different species. Root exudation is part of the rhizodeposition process, which is a major source of soil organic carbon released by plant roots which clearly represents a significant carbon cost to the plant. Root exudates also play a role in soil nutrient availability by altering soil chemistry and soil biological processes. Different studies have highlighted that the rhizosphere soil surrounded by plant roots is more abundant in microbes than the nonrhizosphere soils. Therefore, the major responses in the interaction between plants and microbes must happen in that limited zone. Plants respond to the presence of microbes by releasing a mixture of phytochemicals, volatiles, and high-molecular-weight compounds. Soil microbes, on the other hand, modulate the secretion of root exudates to positively regulate plant growth and disease resistance. Several negative interactions are mediated by root exudates including antimicrobial, biofilm inhibitors, and quorum-sensing mimics to prevent soil-borne pathogens. There is a need to understand these rhizospheric multitrophic interactions in the realistic field conditions to improve the plant growth at species and community level. In addition, studies should be conducted in the field C. De-la-Peña

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Exploiting New Systems-Based Strategies to Elucidate Plant-Bacterial Interactions in the Rhizosphere

Cited by 91 publications

References 68 publications

Plant responses to plant growth-promoting rhizobacteria

Plant responses to plant growth-promoting rhizobacteria

Root-Microbe Communication through Protein Secretion

Plant Root Secretions and Their Interactions with Neighbors

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