Exploiting salivary <i>miR‐31</i> as a clinical biomarker of oral squamous cell carcinoma

Liu, Chung Ji; Lin, Shu Chun; Yang, Cheng Chieh; Cheng, Hui; Chang, Kuo Wei

doi:10.1002/hed.21713

Cited by 217 publications

(200 citation statements)

References 28 publications

Supporting

Mentioning

182

Contrasting

Unclassified

Order By: Relevance

“…64,128 Moreover, plasma miRNAs are shown to be reduced after tumor resection, implying that they may be released from cancerous tissues into circulation and hence is a potential marker for disease progression. 62,64 This was seen in case of plasma and salivary miR-31 47 and plasma miR-184. 62 …”

Section: Mirnas As Predictive Biomarkers In Oral Carcinomamentioning

confidence: 81%

Impact of microRNA dynamics on cancer hallmarks: An oral cancer scenario

Manasa

Kannan

2017

Tumour Biol.

View full text Add to dashboard Cite

MicroRNAs are endogenous small noncoding RNAs that negatively regulate gene expression at posttranscriptional level. The discovery of microRNAs has identified a new layer of gene regulation mechanisms, which play a pivotal role in development as well as in various cellular processes, such as proliferation, differentiation, cell growth, and cell death. Deregulated microRNA expression favors acquisition of cancer hallmark traits as well as transforms the tumor microenvironment, leading to tumor development and progression. Many recent studies have revealed altered expression of microRNAs in oral carcinoma with several microRNAs shown to have key biological role in tumorigenesis functioning either as tumor suppressors or as tumor promoters. MicroRNA expression levels correlate with clinicopathological variables and have a diagnostic and prognostic value in oral carcinoma. For these reasons, microRNA has been a hot topic in oral cancer research for the last few years. In this review, we attempt to summarize the present understanding of microRNA deregulation in oral carcinoma, their role in acquiring cancer hallmarks, and their potential diagnostic and prognostic value for oral cancer management.

show abstract

Section: Mirnas As Predictive Biomarkers In Oral Carcinomamentioning

confidence: 81%

Impact of microRNA dynamics on cancer hallmarks: An oral cancer scenario

Manasa

Kannan

2017

Tumour Biol.

View full text Add to dashboard Cite

show abstract

“…Park and others 6 found two miRNAs (miR-125a and miR-200a) that had significantly reduced levels in the saliva of oral cancer patients compared to controls and found that these two markers could be used for detecting OSCC. Liu and others 30 recently discovered that salivary miRNA-31 (miR-31) was significantly elevated in patients with oral cancer at all stages of disease and tumor size. This study also determined that salivary miR-31 was more abundant than blood miR-31, indicating oral tumor origin of this biomarker.…”

Section: Salivary Transcriptomementioning

confidence: 99%

Salivary Diagnostics

Wong

2012

Operative Dentistry

View full text Add to dashboard Cite

The past 10 years have witnessed significant advances in the science and technology of salivary diagnostics. The integration of salivary diagnostics into the clinical practice of dentistry is on the horizon to enable chair-side detection of oral and systemic disorders in the dental office. SUMMARYSaliva is a noninvasive and accessible biofluid that permits early detection of oral and systemic diseases. Recent scientific and technologic advances have uncovered specific salivary biomarkers for a number of clinical conditions, including cancers, autoimmune diseases, and cardiovascular disorders. The availability of highly sensitive and highthroughput assays such as microarray, mass spectrometry, reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and nano-scale sensors that can measure proteins and nucleic acids are poising saliva as an emerging biofluid for translational and clinical applications. This paper will discuss development of salivary biomarkers for the detection of oral and systemic diseases and the translational application of these markers for clinical applications.

show abstract

“…The most accessible of these in HNSCC patients are plasma and saliva. Several studies have focused on miRNA profiling saliva of oral cavity tumour patients in the search for a diagnostic tool, with promising results [70,71]. Liu and colleagues compared miRNA detection in both plasma and saliva in oral cavity tumours, finding expression levels to be higher in saliva [70].…”

Section: Detection Of Micrornas In Body Fluidsmentioning

confidence: 99%

“…Several studies have focused on miRNA profiling saliva of oral cavity tumour patients in the search for a diagnostic tool, with promising results [70,71]. Liu and colleagues compared miRNA detection in both plasma and saliva in oral cavity tumours, finding expression levels to be higher in saliva [70]. The non-invasive collection of body fluids such as saliva for measurement of miRNA expression will provide a useful avenue for miRNA profiling, and diagnostic or prognostic tool development, in the future.…”

Section: Detection Of Micrornas In Body Fluidsmentioning

confidence: 99%

“…Focus on the search for a screening tool is shifting to saliva as a readily accessible body fluid, and although research lacks behind that of other human malignancies, there is promise in the search for an accurate screening tool [70,112]. To determine whether a panel of miRNAs can be utilised as a screening tool, the panel identified in tumours would first need to be compared with a cohort of normal tissue, such as normal tonsillar tissue.…”

Section: Diagnosticsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluating cellular microRNA expression in human papillomavirus associated head and neck squamous cell carcinoma

Emmett¹

View full text Add to dashboard Cite

Squamous cell carcinoma of mucosal head and neck sites (HNSCC) is the sixth most common cause of cancer world-wide, and is associated with a significant morbidity and mortality despite advances in therapeutic options over the past decades. HNSCC includes tumours of the oral cavity, oropharynx, larynx and hypopharynx. While there has been a decline in tumours occurring as a result of exposure to the traditional risk factors of tobacco smoking and alcohol consumption, a significant increase has been observed in the number of tumours attributed to the Human Papillomavirus (HPV). This has primarily been associated with causation of tumours in the oropharynx, however, a smaller number of tumours at other subsites are also likely to be related to the virus. Although HPV-related tumours tend to have an improved prognosis and a better response to chemo-and radiotherapy, the pathobiology underlying these differences is not yet fully understood.MiRNAs are short, non-coding single strands of RNA which regulate gene expression at the posttranscriptional level. They have been identified to be dysregulated and function as tumour suppressor or oncogenes in a number of human cancers, including head and neck cancers. MiRNAs have also been associated with prognostication including associations with recurrence of disease and diseasefree survival. As they are readily available across a range of body tissues including solid tissue, saliva, and plasma, these biomarkers have potential utility in relation to screening for disease diagnosis, recurrence or progression. Although there is an established difference between HPV-positive and negative tumours in the oropharynx clinically, the majority of studies investigating miRNA expression in HNSCC have not taken this factor into account. In the small number of studies which have examined the effect of HPV on miRNA expression in HNSCC, there is a lack of consistency in the results described.This thesis aimed to assess the difference in miRNA expression between HPV-positive and negative tumours, through use of miRNA microarray followed by validation with real-time PCR using TaqMan assays combined with a sensitive detection method (Fluidigm HD) in a larger cohort of patients. It also investigated differences in miRNA expression in association with lifestyle factors, demographics and clinical outcomes. A set of seven miRNAs was identified to be differentially expressed between HPV-positive and negative tumours in the oropharynx. No difference in miRNA expression was observed between the two tumour cohorts at other subsites in the head and neck.Differences in miRNA expression were also observed with lifestyle factors such as smoking status and alcohol consumption. Recurrence of the primary tumour and overall disease-free survival were further associated with altered miRNA expression. The findings outlined in this thesis add to the present literature regarding differences in miRNA expression between HPV-positive and negative iii tumours in the oropharynx, and provide a basis for further...

show abstract

Exploiting salivary miR‐31 as a clinical biomarker of oral squamous cell carcinoma

Abstract: Our results point to a potential application of salivary miR-31 as a biomarker for early detection and postoperative follow-up of oral carcinoma.

Cited by 217 publications

References 28 publications

Impact of microRNA dynamics on cancer hallmarks: An oral cancer scenario

Impact of microRNA dynamics on cancer hallmarks: An oral cancer scenario

Salivary Diagnostics

Evaluating cellular microRNA expression in human papillomavirus associated head and neck squamous cell carcinoma

Contact Info

Product

Resources

About