Exploiting synergistic effect of CO/NO gases for soft tissue transplantation using a hydrogel patch

Abstract

Autologous skin flap transplantation is a common method for repairing complex soft tissue defects caused by cancer, trauma, and congenital malformations. Limited blood supply range and post-transplantation ischemia-reperfusion injury can lead to distal necrosis of the flap and long-term functional loss, which severely restricts the decision-making regarding the optimal surgical plan. To address this issue, we develop a hydrogel patch that releases carbon monoxide and nitric oxide gases on demand, to afford a timely blood supply for skin flap transplantation during surgery. Using an ischemia-reperfusion dorsal skin flap model in rats, we show that the hydrogel patch maintains the immediate opening of blood flow channels in transplanted tissue and effective blood perfusion throughout the perioperative period, activating perfusion of the hemodynamic donor site. We demonstrate that the hydrogel patch promotes distal vascularization and long-term functional reconstruction of transplanted tissues by inhibiting inflammatory damage and accelerating blood vessel formation.

Site-specific albumin tagging with chloride-containing near-infrared cyanine dyes: molecular engineering, mechanism, and imaging applications

“…The Cl-containing cyanine dyes for albumin tagging have shown promising applications in terms of cancer imaging and imaging-guided surgery. 104 The covalent binding between the dyes and albumin allows for enhanced permeability and retention at the tumor site, making them suitable for tumor imaging. The brightness and photostability of the dye-albumin complexes have been significantly improved, enabling long-term bioimaging (such as imaging of sentinel lymph nodes).…”

Biomimetic NIR-II fluorescent proteins created from chemogenic protein-seeking dyes for multicolor deep-tissue bioimaging

“…Due to the diversity of vascular dissection and complexity of perforator flap design, postoperative under perfusion can often result in local necrosis caused by ischemia and hypoxia, making flap necrosis the most common and serious complication of skin flap surgery [48][49][50] . Unfortunately, there are currently no appropriate imaging techniques for accurate and noninvasive monitoring of flap functionality.…”

A Single‐Component Dual Donor Enables Ultrasound‐Triggered Co‐release of Carbon Monoxide and Hydrogen Sulfide

“…Due to the co‐existence and subtle interplay of different gasotransmitters in both physiological and pathological conditions, the co‐delivery of multiple gasotransmitters has received increasing attention, representing an emerging direction of gas therapy [4] . In this regard, several recent contributions from Chen, [5] Yang, [6] and other research groups, [7] simultaneously incorporated two kinds of gasotransmitter donors into various nanocarriers. These designs enabled the co‐release of two kinds of gasotransmitters, exhibiting enhanced therapeutic effects in angiogenesis, wound healing, and tumor immunotherapy.…”