Exploiting the bioactive properties of γ-oryzanol from bran of different exotic rice varieties

Castanho, Ana; Lageiro, Manuela; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Soković, Marina; Cunha, Luís Miguel; Brites, Carla

doi:10.1039/c8fo02596g

Cited by 29 publications

(32 citation statements)

References 38 publications

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“…GO was extracted from bran with the isopropanol solvent (Castanho et al, 2019). To determine the recovery of the GO, spiking experiments were performed with known standard content before extraction.…”

Section: Extraction Methodsmentioning

confidence: 99%

“…Five to eight standard solutions of GO were prepared according to Castanho et al (2019), using an injection volume of 20 µL from each solution (in triplicate) to obtain the 4 peaks chromatograms.…”

Section: Validation Of the Hplc Methodsmentioning

confidence: 99%

“…GO quantification was performed by RP-HPLC coupled to a photodiode array detector (PDA) (Castanho et al, 2019).…”

Section: Go Quantification By Hplcmentioning

confidence: 99%

“…The cytotoxic properties of GO have also been explored in tumour-bearing mice (Kim et al, 2012), showing positive effects. In a recent study, Castanho et al (2019) tested rice bran lipid extracts from different origins and pericarp colours against four human tumour cell lines (NCI-H460, HeLa, HepG2, and MCF -7); the results showed the effectiveness of the extracts and also a significant negative correlation between GO concentration and GI 50 values for HeLa, HepG2 and MCF7.…”

Section: Introductionmentioning

confidence: 99%

“…GO extraction methods and extracting solvents are considered the key factors influencing the extraction efficiency of the bioactive compounds-rich extracts, due to the chemical instability and complexity of ferulates of triterpene alcohols and sterols and it may be the cause of the high variability detected in the published data with range spanning from 0.6 g/Kg to 9.1 g/kg of rice bran (Castanho et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Gamma Oryzanol Variability, an Attractive Rice Bran Bioactive Compound

et al. 2020

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Gamma-oryzanol is a compound present on rice bran, which has been studied for its bioactive properties, including its cytotoxic activity. The gamma-oryzanol biosynthesis occurs on the lipidic membrane of the plant and can be influenced by edaphoclimatic grown conditions. Two Portuguese rice varieties (Ceres and Maçarico) from 6 growing environments were analysed in order to explore the influence of the growing conditions on the gamma-oryzanol content, its constituents, and cytotoxic effect against 4 human tumour cell lines (NCI-H460, HeLa, HepG2, and MCF -7). The overall variability of gamma-oryzanol concentration was from 1.56 g / Kg to 3.19 g / Kg, Ceres reveals higher values than Maçarico; those concentrations also varied with the growing environment. The environment was also determinant for the individual concentration of the 3 gamma-oryzanol compounds (cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate). A cytotoxic effect was observed for all the tested tumour cell lines and also varied with the growing environment.

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Section: Extraction Methodsmentioning

confidence: 99%

Section: Validation Of the Hplc Methodsmentioning

confidence: 99%

“…GO quantification was performed by RP-HPLC coupled to a photodiode array detector (PDA) (Castanho et al, 2019).…”

Section: Go Quantification By Hplcmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Gamma Oryzanol Variability, an Attractive Rice Bran Bioactive Compound

et al. 2020

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Study of the thermal stability of γ‐oryzanol present in rice bran oil over time

et al. 2021

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BACKGROUND: Rice bran oil is unique among edible oils owing to its rich source of commercially and nutritionally important phytochemicals, such as oryzanol. γ-Oryzanol performs an important role in the stability of rice bran oil. The crude rice bran oil obtained by solvent extraction is subjected to either chemical or physical refining to meet the specifications of edible-grade vegetable oil. These refining processes can cause the compounds present in rice bran oil to degrade. The aim of this study was to evaluate the stability of γ-oryzanol present in chemically and physically refined rice bran oils, when submitted to temperatures of 100, 140, and 180°C for a period of 1368 h. RESULTS:The chemically refined rice bran oil presented a lower γ-oryzanol content than the physically refined rice bran oil at all heating temperatures. The losses of γ-oryzanol at 100°C, 140°C, and 180°C at the end of the heating periods for the chemically refined oil were 53.47%, 58.48%, and 97.05% respectively, and for the physically refined oil the losses were 38.11%, 53.58%, and 91.11% respectively. CONCLUSION: Based on the results of the time to reduce the oryzanol concentration by 50% and 100%, it is observed that the oil of rice meal refined physically presents greater stability, in the different temperatures studied and over time, than the oil of rice meal refined chemically. Thus, for situations where the oil needs to be subjected to prolonged heating, a temperature of 100°C is indicated. In this condition, the physically refined oil is better for maintaining a higher concentration of γ-oryzanol.

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Rice Bran Lipidome Identifies Novel Phospholipids, Glycolipids, and Oxylipins with Roles in Lipid Metabolism of Hypercholesterolemic Children

Santos

Barouh

Lullien-Pellerin

et al. 2023

Molecular Nutrition Food Res

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Scope: The purpose of the study is to characterize the chemical diversity in rice bran (RB) lipidome and determines whether daily RB consumption for 4 weeks may modulate plasma lipid profiles in children. Methods and results: Untargeted and targeted lipidomics via ultra-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UPLC-MS/MS) are applied to identify bioactive RB lipids from a collection of 17 rice varieties. To determine the impact of RB (Calrose-USA variety) supplementation on plasma lipid profile, a secondary analysis of plasma lipidome is conducted on data recorded in a clinical study (NCT01911390, n = 18 moderately hypercholesterolemic children) before and after 4 weeks of dietary intervention with a control or RB supplemented (15 g day −1 ) snack. Untargeted lipidomic reveals 118 lipids as the core of lipidome across all varieties among which phospholipids are abundant and oxylipins present. Phytoprostanes and phytofurans are quantified and characterized. Lipidome analysis of the children plasma following RB consumption reveals the presence of polar lipids and oxylipins alongside putative modulations in endocannabinoids associated with RB consumption. Conclusion: The investigation of novel polar lipids, oxylipins, phytoprostanes, and phytofurans in RB extracts provides support for new health-promoting properties interesting for people at risk for cardiometabolic disease.

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Exploiting the bioactive properties of γ-oryzanol from bran of different exotic rice varieties

Abstract: Analysing nine rice bran extracts with different γ-oryzanol concentrations revealed cytotoxic effects on four human tumour cell lines.

Cited by 29 publications

References 38 publications

Assessment of Gamma Oryzanol Variability, an Attractive Rice Bran Bioactive Compound

Assessment of Gamma Oryzanol Variability, an Attractive Rice Bran Bioactive Compound

Study of the thermal stability of γ‐oryzanol present in rice bran oil over time

Rice Bran Lipidome Identifies Novel Phospholipids, Glycolipids, and Oxylipins with Roles in Lipid Metabolism of Hypercholesterolemic Children

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