Exploiting the Genetic Diversity of Maize Using a Combined Metabolomic, Enzyme Activity Profiling, and Metabolic Modeling Approach to Link Leaf Physiology to Kernel Yield

Cañas, Rafael A.; Yesbergenova-Cuny, Zhazira; Simons, Margaret; Chardon, Fabien; Armengaud, Patrick; Quilleré, Isabelle; Cukier, Caroline; Gibon, Yves; Limami, Anis M.; Nicolas, Stéphane; Brulé, Lénaïg; Lea, Peter J.; Maranas, Costas D.; Hirel, Bertrand

doi:10.1105/tpc.16.00613

Cited by 62 publications

(70 citation statements)

References 111 publications

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“…Plant response to N availability has been widely studied in Arabidopsis as well as in crop species, most often focusing only on ample, limiting, and scarcity conditions [14,16,19,37,38]. With this study, we quantified the Arabidopsis response to an ampler set of nutrition regimes, ranging from 0.01 mM up to 50 mM NO 3 , taking into account genotype features.…”

Section: Discussionmentioning

confidence: 99%

Impact of the Genetic–Environment Interaction on the Dynamic of Nitrogen Pools in Arabidopsis

et al. 2018

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Abstract:Mineral nutrient availability and in particular nitrogen abundance has a huge impact on plant fitness and yield, so that plants have developed sophisticated adaptive mechanisms to cope with environmental fluctuations. The vast natural variation existing among the individuals of a single species constitutes a great potential to decipher complex traits such as nutrient use efficiency. By using natural accessions of Arabidopsis thaliana that differ for their pattern of adaptation to nitrogen stress, we investigated the plant response to nitrate supplies ranging from 0.01 mM up to 50 mM nitrate. The biomass allocation and the different nitrogen pools in shoot and in roots were monitored to establish the nutrition status of each plant. Analysis of variation for these traits revealed genetic differences between accessions for their sensibility to nitrate availability and for their capacity to produce shoot biomass with the same nitrogen nutrition index. From the correlation matrix of all traits measured, a statistical model was formulated to predict the shoot projected area from the nitrate supply. The proposed model points out the importance of genetic variation with respect to the correlation between root thickness and amino acids content in roots. The model provides potential new targets in plant breeding for nitrogen use efficiency.

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Section: Discussionmentioning

confidence: 99%

Impact of the Genetic–Environment Interaction on the Dynamic of Nitrogen Pools in Arabidopsis

et al. 2018

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“…Using systems biology approaches, attempts have been made to integrate the different “omics” studies in order to distinguish regulatory networks. Ultimately, this has allowed the identification of certain biological components involved in the control of plant productivity, notably vegetative biomass and grain yield, as well as resistance to biotic and abiotic stresses (Cañas et al., ; Fukushima, Kanaya, & Nishida, ; Sheth & Thaker, ).…”

Section: Introductionmentioning

confidence: 99%

Labeling Maize (Zea mays L.) Leaves with ¹⁵NH₄⁺ and Monitoring Nitrogen Incorporation into Amino Acids by GC/MS Analysis

et al. 2018

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The human body contains approximately 3.2% nitrogen (N), mainly present as protein and amino acids. Although N exists at a high concentration (78%) in the air, it is not readily available to animals and most plants. Plants are however able to take up both nitrate (NO ) and ammonium (NH ) ions from the soil and convert them to amino acids and proteins, which are excellent sources for all animals. Most N is available as the stable isotope N, but a second form, N, is present in very low concentrations. N can be detected in extracts of plants by gas chromatography followed by mass spectrometry (GC/MS). In this protocol, the methods are described for tracing the pathway by which plants are able to take up N-labeled nitrate and ammonium and convert them into amino acids and proteins. A protocol for extracting and quantifying amino acids and N enrichment in maize (Zea mays L.) leaves labeled with NH is described. Following amino acid extraction, purification, and separation by GC/MS, a calculation of the N enrichment of each amino acid is carried out on a relative basis to identify any differences in the dynamics of amino acid accumulation. This will allow a study of the impact of genetic modifications or mutations on key reactions involved in primary nitrogen and carbon metabolism. © 2018 by John Wiley& Sons, Inc.

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“…Exploiting the Genetic Diversity of Maize Using a Combined Metabolomic, Enzyme Activity Profiling, and Metabolic Modeling Approach to Link Leaf Physiology to Kernel Yield.Correlation studies and metabolic network analyses allowed the description of a maize ideotype with a high grain yield potential. Such an ideotype is characterized by low accumulation of soluble amino acids and carbohydrates in the leaves and high activity of enzymes involved in the C4 photosynthetic pathway and in the biosynthesis of amino acids derived from glutamate 69 .…”

Section: Introductionmentioning

confidence: 99%

Emerging Applications of Fermented Fruit Enzyme Beverages as Nutraceutical, Functional Food, and Designer Food Products: A Review

Kumar¹,

Saini²,

Meenakshi³

et al. 2018

J. Pharm. Sci. Innov.

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In the past, the beneficial effects of fermented fruits were unknown, and so people primarily used fermentation to preserve fruits, enhance shelf life, and improve flavor. Fermentation of fruits has been adopted over many generations, primarily due to their commercial significance. Edible tropical fruits such as, cashew apple, mangoes, papaya, pineapple, litchi, guava, bael, banana, pomegranate, jamun and palm etc. with high export potential have their origin in the tropics and require rather suitable climatic conditions; and can not tolerate environmental stress. Most of the tropical fruits are important sources of antioxidants, vitamins and minerals and form a very healthy part of a diet. An effective utilisation of inferior grade and over-ripe fruits and processing them into fermented beverages has been revealed as a new and promising alternative to generate extra revenues whilst conducting a sustainable exploitation of wastes. In addition, microorganisms contributing to the fermentation process have recently been associated with many health benefits, and so these microorganisms have become another focus of attention. This paper reviews the production techniques and beneficial health effects of different fermented fruits. Scientific advances in the emerging area of functional beverages; as they provide a health benefit beyond the basic nutritional functions also reported in this paper.

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Exploiting the Genetic Diversity of Maize Using a Combined Metabolomic, Enzyme Activity Profiling, and Metabolic Modeling Approach to Link Leaf Physiology to Kernel Yield

Cited by 62 publications

References 111 publications

Impact of the Genetic–Environment Interaction on the Dynamic of Nitrogen Pools in Arabidopsis

Impact of the Genetic–Environment Interaction on the Dynamic of Nitrogen Pools in Arabidopsis

Labeling Maize (Zea mays L.) Leaves with ¹⁵NH₄⁺ and Monitoring Nitrogen Incorporation into Amino Acids by GC/MS Analysis

Emerging Applications of Fermented Fruit Enzyme Beverages as Nutraceutical, Functional Food, and Designer Food Products: A Review

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Exploiting the Genetic Diversity of Maize Using a Combined Metabolomic, Enzyme Activity Profiling, and Metabolic Modeling Approach to Link Leaf Physiology to Kernel Yield

Cited by 62 publications

References 111 publications

Impact of the Genetic–Environment Interaction on the Dynamic of Nitrogen Pools in Arabidopsis

Impact of the Genetic–Environment Interaction on the Dynamic of Nitrogen Pools in Arabidopsis

Labeling Maize (Zea mays L.) Leaves with 15NH4+ and Monitoring Nitrogen Incorporation into Amino Acids by GC/MS Analysis

Emerging Applications of Fermented Fruit Enzyme Beverages as Nutraceutical, Functional Food, and Designer Food Products: A Review

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Labeling Maize (Zea mays L.) Leaves with ¹⁵NH₄⁺ and Monitoring Nitrogen Incorporation into Amino Acids by GC/MS Analysis