Exploiting the synergism among physical and chemical processes for improving food safety

Berdejo, Daniel; Pagán, Elisa; García-Gonzalo, Diego; Pagán, Rafael

doi:10.1016/j.cofs.2018.08.004

Cited by 24 publications

(16 citation statements)

References 56 publications

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“…Antibiotic resistance is a global health threat associated with increased morbidity, mortality, hospitalization, and healthcare costs ( Laxminarayan et al, 2013 ; Frieri et al, 2017 ; Klein et al, 2019 ). There is thus high motivation for development of novel approaches combining multiple antibacterial strategies that have different modes of action ( Alexopoulos et al, 2019 ; Berdejo et al, 2019 ; Douafer et al, 2019 ; Juma et al, 2020 ). Among the promising complementary techniques for such approaches is electroporation, in which exposure of bacteria to short electric pulses of sufficient strength permeabilizes their membranes, thereby facilitating the uptake of diverse compounds, including antibiotics ( Garner, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Electroporation alone can also cause bacterial death, but the levels of inactivation typically achieved are insufficient for general stand-alone use, providing motivation for development of synergistic treatments ( Martín-Belloso and Sobrino-López, 2011 ; Berdejo et al, 2019 ). For food and beverage preservation, the combination of electroporation with antibacterials has been shown to decrease required pulse amplitudes and increase achievable inactivation rates ( Berdejo et al, 2019 ). However, the range of antibacterials permissible for such applications is in practice limited to substances that either occur also naturally in food (e.g., acetic acid, citric acid, lactic acid, cinnamaldehyde, and linalool) or are registered as food additives (e.g., nisin, triethyl citrate).…”

Section: Introductionmentioning

confidence: 99%

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Electroporation as an Efficacy Potentiator for Antibiotics With Different Target Sites

2021

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Antibiotic resistance is a global health threat, and there is ample motivation for development of novel antibacterial approaches combining multiple strategies. Electroporation is among the promising complementary techniques – highly optimizable, effective against a broad range of bacteria, and largely impervious to development of resistance. To date, most studies investigating electroporation as an efficacy potentiator for antibacterials used substances permissible in food industry, and only few used clinical antibiotics, as acceptable applications are largely limited to treatment of wastewaters inherently contaminated with such antibiotics. Moreover, most studies have focused mainly on maximal achievable effect, and less on underlying mechanisms. Here, we compare Escherichia coli inactivation potentiation rates for three antibiotics with different modes of action: ampicillin (inhibits cell wall synthesis), ciprofloxacin (inhibits DNA replication), and tetracycline (inhibits protein synthesis). We used concentrations for each antibiotic from 0 to 30× its minimum inhibitory concentration, a single 1-ms electric pulse with amplitude from 0 to 20 kV/cm, and post-pulse pre-dilution incubation either absent (≲1 min) or lasting 60 min, 160 min, or 24 h. Our data show that with incubation, potentiation is significant for all three antibiotics, increases consistently with pulse amplitude, and generally also with antibiotic concentration and incubation time. With incubation, potentiation for ampicillin was rather consistently (although with weak statistical significance) superior to both ciprofloxacin and tetracycline: ampicillin was superior to both in 42 of 48 data points, including 7 with significance with respect to both, while at 60- and 160-min incubation, it was superior in 31 of 32 data points, including 6 with significance with respect to both. This suggests that electroporation potentiates wall-targeting antibiotics more than those with intracellular targets, providing motivation for in-depth studies of the relationship between the mode of action of an antibiotic and its potentiation by electroporation. Identification of substances permissible in foods and targeting the cell wall of both Gram-negative and Gram-positive bacteria might provide candidate antibacterials for broad and strong potentiation by electroporation applicable also for food preservation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electroporation as an Efficacy Potentiator for Antibiotics With Different Target Sites

2021

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“…A combination of different UV‐LED wavelengths has been suggested as a hurdle approach for enhancing microbial decontamination efficacy (Figure 3a) (Chevremont et al., 2012; Nakahashi et al., 2014b). In this context, such treatment applied either simultaneously and/or sequentially has resulted in additive, antagonistic, or synergistic effects (Berdejo, Pagán, García‐Gonzalo, & Pagán, 2019). Recent studies conducted on the effect of combined UV‐LEDs wavelength for the treatment of microorganisms are summarized in Table 4.…”

Section: Combination Of Different Uv‐leds At Various Wavelengths For mentioning

confidence: 99%

Recent advances on the application of UV‐LED technology for microbial inactivation: Progress and mechanism

Kebbi

Muhammad

Sant’Ana

et al. 2020

Comp Rev Food Sci Food Safe

101

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Conventional technologies for the inactivation of microorganisms in food products have their limitations, especially changes in quality attributes that have led to quality deterioration, low consumer acceptance, impact on the environment, and potential health hazards (carcinogens). Ultraviolet (UV) light is an emerging promising nonthermal technology employed for microbial inactivation in water, liquid, and solid food products to curtail the limitations above. This review provides an insight into UV light-emitting diodes (UV-LEDs)' potential as an alternative to the traditional UV lamps for microbial inactivation in liquid and solid media. Also, the mechanisms of inactivation of lone and combined UVA-, UVB-, and UVC-LEDs were discussed. The strategies utilized to improve the efficacy between the UV-LED treatments at various wavelengths were summarized. Combining different UV-LEDs treatments at different wavelengths have a synergistic effect and suppression of microbial cell reactivation. The UV-LED-based advanced oxidation processes (AOPs) also have high germicidal action against numerous microorganisms and are efficient for the degradation of micropollutants. Among the UV-LEDs discussed, UVC-LED has the most antimicrobial effect with the most efficient micropollutants decomposition with regards to UV-LED-based AOPs. This review has provided vital information for future application, development, and customization of UV-LED systems that can meet the food and water safety requirements and energy efficiency.

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“…As a result, other food processing methods such as hydrostatic pressure techniques [ 28 ], cold plasma [ 29 ], ultraviolet light [ 30 ], ultrasound [ 31 ] or pulsed electric fields [ 32 ] have been extensively focused on for their use in the improvement of food safety. Nevertheless, the best effects are expected when a synergism between various methods is acquired [ 33 ]. Therefore, in order to improve the efficacy of nisin and induce a synergistic response, methods affecting the cell wall and plasma membrane are required.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields

et al. 2021

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Foodborne pathogens are frequently associated with risks and outbreaks of many diseases; therefore, food safety and processing remain a priority to control and minimize these risks. In this work, nisin-loaded magnetic nanoparticles were used and activated by alternating 10 and 125 mT (peak to peak) magnetic fields (AMFs) for biocontrol of bacteria Listeria innocua, a suitable model to study the inactivation of common foodborne pathogen L. monocytogenes. It was shown that L. innocua features high resistance to nisin-based bioactive nanoparticles, however, application of AMFs (15 and 30 min exposure) significantly potentiates the treatment resulting in considerable log reduction of viable cells. The morphological changes and the resulting cellular damage, which was induced by the synergistic treatment, was confirmed using scanning electron microscopy. The thermal effects were also estimated in the study. The results are useful for the development of new methods for treatment of the drug-resistant foodborne pathogens to minimize the risks of invasive infections. The proposed methodology is a contactless alternative to the currently established pulsed-electric field-based treatment in food processing.

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Exploiting the synergism among physical and chemical processes for improving food safety

Cited by 24 publications

References 56 publications

Electroporation as an Efficacy Potentiator for Antibiotics With Different Target Sites

Electroporation as an Efficacy Potentiator for Antibiotics With Different Target Sites

Recent advances on the application of UV‐LED technology for microbial inactivation: Progress and mechanism

Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields

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