Exploration and optimization of conditions for quantitative analysis of lignans in <i>Schisandra chinensis</i> by an online supercritical fluid extraction with supercritical fluid chromatography system

Dai, Zhuoshun; Xin, Huaxia; Fu, Qing; Hao, Hongyuan; Li, Qiang; Liu, Qiaoxia; Jin, Yu

doi:10.1002/jssc.201900222

Cited by 25 publications

(3 citation statements)

References 23 publications

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“…The lignans present in this plant material are free aglycones, with most of them having little to no hydroxyl groups, making them rather lipophilic. Many papers optimizing the Schisandra lignans' SFE extraction were published, with two of them recently [84,85]. Perhaps the most important point from these works is that temperature and pressure variations have a lesser effect on extractability than the addition of methanol or ethanol to carbon dioxide, which dramatically increased the lignan yield [103].…”

Section: Methodsmentioning

confidence: 99%

Extraction Techniques and Analytical Methods for Isolation and Characterization of Lignans

Patyra

Kołtun-Jasion

Jakubiak

et al. 2022

Plants

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Lignans are a group of natural polyphenols present in medicinal plants and in plants which are a part of the human diet for which more and more pharmacological activities, such as antimicrobial, anti-inflammatory, hypoglycemic, and cytoprotective, are being reported. However, it is their cytotoxic activities that are best understood and which have shed light on this group. Two anticancer drugs, etoposide, and teniposide, were derived from a potent cytotoxic agent—podophyllotoxin from the roots of Podophyllum peltatum. The evidence from clinical and observational studies suggests that human microbiota metabolites (enterolactone, enterodiol) of dietary lignans (secoisolariciresinol, pinoresinol, lariciresinol, matairesinol, syringaresinol, medioresinol, and sesamin) are associated with a reduced risk of some hormone-dependent cancers. The biological in vitro, pharmacological in vivo investigations, and clinical studies demand significant amounts of pure compounds, as well as the use of well-defined and standardized extracts. That is why proper extract preparation, optimization of lignan extraction, and identification are crucial steps in the development of lignan use in medicine. This review focuses on lignan extraction, purification, fractionation, separation, and isolation methods, as well as on chromatographic, spectrometric, and spectroscopic techniques for their qualitative and quantitative analysis.

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Section: Methodsmentioning

confidence: 99%

Extraction Techniques and Analytical Methods for Isolation and Characterization of Lignans

Patyra

Kołtun-Jasion

Jakubiak

et al. 2022

Plants

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“…Health food contain-ing schisandra mainly includes improving sleep, auxiliary protection against chemical liver injury, enhancing immunity, relieving physical fatigue and anti-aging, all of which are consistent with the traditional benefits of schisandra [10]. Schisandra B (Sch B) is a lignan compound isolated from Schisandra chinensis (Turcz) Baill [11,12]. Sch B has antiinflammatory, liver-protecting, anti-renal toxicity and anti-cancer activities [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Schisandrin B Attenuates Hepatic Stellate Cell Activation and Promotes Apoptosis to Protect against Liver Fibrosis

Zhao

Xia

et al. 2021

Molecules

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The activation of hepatic stellate cells (HSC) plays a key role in the progression of hepatic fibrosis, it is essential to remove activated HSC through apoptosis to reverse hepatic fibrosis. Schisandrin B (Sch B) is the main chemical component of schisandrin lignan, and it has been reported to have good hepatoprotective effects. However, Schisandrin B on HSC apoptosis remains unclear. In our study, we stimulated the HSC-T6 and LX-2 cell lines with TGF-β1 to induce cell activation, and the proliferation and apoptosis of the activated HSC-T6 and LX-2 cells were detected after treatment with different doses of Schisandrin B. Flow cytometry results showed that Sch B significantly reduced the activity of activated HSC-T6 and LX-2 cells and significantly induced apoptosis. In addition, the cleaved-Caspase-3 levels were increased, the Bax activity was increased, and the Bcl-2 expression was decreased in HSC-T6 and LX-2 cells treated with Sch B. Our study showed that Sch B inhibited the TGF-β1-induced activity of hepatic stellate cells by promoting apoptosis.

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“…Schisandrin B (SchB) is one of the lignans with high content from Schisandra chinensis 1 . SchB has been reported to have a variety of pharmacological activities, including anti-inflammation 2 , antioxidation 3 , anti-cancer 4 , 5 etc.…”

Section: Introductionmentioning

confidence: 99%

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis

Wang

Liao

Zhang

et al. 2020

Sci Rep

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Schisandrin B (SchB) is the highest content of biphenyl cyclooctene lignans in Schisandra chinensis. It has been reported to have a variety of pharmacological effects, including anti-inflammatory, anti-oxidant, anti-cancer, heart protection, liver protection. In this study, we found that SchB can promote the proliferation of MC3T3-E1 subclone 14 cells. Meanwhile, we found that SchB can regulate the BMP2-SMADs signaling pathway by increasing gene and protein expression of those relative biomolecules. Furthermore, SchB can raise the RUNX2 and SP7 expression in both mRNA and protein levels. Since the role of BMP2-SMADs-RUNX2-SP7 signaling axis in osteoblast proliferation and differentiation has been well documented. The present experimental findings indicate that SchB could promote the proliferation and differentiation of osteoblasts through BMP2-SMADs-RUNX2-SP7 signaling axis. Schisandrin B (SchB) is one of the lignans with high content from Schisandra chinensis 1. SchB has been reported to have a variety of pharmacological activities, including anti-inflammation 2 , antioxidation 3 , anti-cancer 4,5 etc. It is worth noting that SchB has been reported to ameliorate chondrocytes inflammation and osteoarthritis by inhibiting NF-κB and MAPK signaling pathways 6. These findings suggest that SchB may play a potential role in alleviate bone disease. However, the effect of SchB on osteoblasts is unknown. MC3T3-E1 subclone 14 cells are often used to study the proliferation and differentiation of osteoblasts 7-9. It is well known BMP2-SMADs signaling pathway play a key role in mediating osteoblast differentiation and osteogenesis 10. While Runx2 and SP7 are transcription factors that are essential for osteoblast proliferation and differentiation 11,12. Here, the effects of SchB on the proliferation of MC3T3-E1 subclone 14 cells were evaluated by MTT assay, and the effects of SchB on the expression of genes and proteins related to BMP2-SMADs-RUNX2-SP7 signaling axis were investigated by quantitative PCR and western blot, respectively. Results SchB promotes MC3T3-E1 subclone 14 cells proliferation in a certain concentration range. Osteoblasts are responsible for bone synthesis, remodeling and healing 13. Osteoblast proliferation plays an important role in bone maintenance and development. In the present study, SchB stimulated the proliferation of MC3T3-E1 subclone 14 cells in gradually increase degrees at the concentration of 1.25-40 μM, while showed cytotoxicity at concentrations of 80 μM and 100 μM. In addition, after 24 h of treatment, the effect of SchB on the proliferation of MC3T3-E1 cells tended to be flat, and the intensity of action did not increase significantly with the extension of time (Fig. 1).

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Exploration and optimization of conditions for quantitative analysis of lignans in Schisandra chinensis by an online supercritical fluid extraction with supercritical fluid chromatography system

Cited by 25 publications

References 23 publications

Extraction Techniques and Analytical Methods for Isolation and Characterization of Lignans

Extraction Techniques and Analytical Methods for Isolation and Characterization of Lignans

Schisandrin B Attenuates Hepatic Stellate Cell Activation and Promotes Apoptosis to Protect against Liver Fibrosis

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis

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