Exploration for anti-insect properties of lectin from seeds of soybean (Glycine max) usingBactrocera cucurbitae as a model

Singh, Kuljinder; Kaur, Manpreet; Rup, Pushpinder J.; Singh, Jatinder

doi:10.1007/bf02981200

Cited by 16 publications

(19 citation statements)

References 25 publications

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“…Plant lectins constitute anti-nutrients in crops which are often assessed in the comparative safety assessments of GM soybean. Lectins are known to play an important role in the defensive and communication systems of plants [36, 37] and are known to affect arthropods [38–41]. …”

Section: Resultsmentioning

confidence: 99%

Are Limits of Concern a useful concept to improve the environmental risk assessment of GM plants?

et al. 2017

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BackgroundThe European Food Safety Authority (EFSA) has introduced a concept for the environmental risk assessment of genetically modified (GM) plants which foresees the definition of ecological threshold values defining acceptable adverse effects of the GM plant on the environment (Limits of Concern, LoC).MethodsWe analysed the LoC concept by scrutinising its feasibility with regard to important aspects of the environmental risk assessment. We then considered its relationship with protection goals, the comparative safety assessment and the stepwise testing approach. We finally discussed its usefulness for assessing long-term effects, effects on non-target organisms and species of conservation concern.ResultsThe LoC concept is a possible approach to introduce ecological thresholds into environmental risk assessment in order to evaluate environmental harm. However, the concept leaves many important aspects open. Thresholds for environmental harm for protection goals need spatial and temporal differentiation from LoCs used for ERA indicators. Regionalisation of LoCs must be provided for as biodiversity levels and protection goals vary across the EU. Further guidance is needed with respect to the consequences, in case LoCs are exceeded and a link needs to be established between environmentally relevant results from the comparative safety assessment and the LoC concept. LoCs for long-term effects have to be evaluated by long-term monitoring. LoCs for non-target organisms need to be discriminated according to the species and parameters assessed.ConclusionsThe overall LoC concept is considered useful if LoCs are further specified and differentiated. Although LoCs will finally be determined by political decisions, they should be based on scientific grounds in order to increase confidence in the conclusions on the safety of GM plants.

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Section: Resultsmentioning

confidence: 99%

Are Limits of Concern a useful concept to improve the environmental risk assessment of GM plants?

et al. 2017

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“…Reduced pupation and emergence observed in the present study is completely in accordance with the results of Singh et al . (,b, ) who reported the detrimental effect of legume lectins from Ricinus communis (galNAc, β‐gal specific), Glycine max (galNac specific) and Erythrina indica ( d ‐galactose specific) on the melon fruit fly B. cucurbitae . Glycine max significantly lowered ( P < 0.01) the pupation and emergence of adult flies at the lower amounts.…”

Section: Discussionmentioning

confidence: 99%

“…Contrary to the present findings, the activity of acid and alkaline phosphatase was found to decrease in the larvae of B. cucurbitae treated with lectins from R. communis and G. max , (Singh et al . ,b).…”

Section: Discussionmentioning

confidence: 99%

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Monitoring growth, survival and enzyme system of melon fruit fly Bactrocera cucurbitae (Coquillett) under the influence of affinity purified pea lectin

Kaur

Sohal

Kaur

et al. 2012

Entomological Science

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Pisum sativum agglutinin has been shown to act as a feeding inhibitor for various insect pests belonging to different orders: Lepidoptera, Coleoptera and Hemiptera. In the present study, its insecticidal activity was assessed through monitoring the growth and development of a dipteran pest Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae). Pea lectin, P. sativum agglutinin (PSA) was purified by single step affinity chromatography on a Sephadex G‐100 and the purification was monitored through hemagglutination activity and SDS‐PAGE. Insect feeding assays were conducted to determine the effect of pea lectin against first and second instar larvae of melon fruit fly B. cucurbitae. Lectin was incorporated in an artificial diet at a varied range of concentrations, 12.5, 25, 50, 100, 200 and 400 μg/mL. The lectin showed highly significant antimetabolic effects in both first and second instars. Time taken for pupation and development as well as percentage pupation and percentage adult emergence were adversely affected. The activity of three hydrolase enzymes (esterases, acid and alkaline phosphatases), five oxidoreductases (superoxide dismutase, catalase, ascorbate peroxidase, peroxidase, O‐demethylase) and one group transfer enzyme (glutathione‐S‐transferases) was also assessed in second instar larvae fed on lectin treated diet at 100 μg/mL concentration. The P. sativum lectin significantly and deleteriously influenced the activity of all these enzymes at all exposure intervals.

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“…While the physiological roles of plant lectins are still not clear (Van Damme et al, 2008), their effects on other organisms have been widely investigated (Vasconcelos and Oliveira, 2004;Van Damme et al, 2008). In general, plant lectins may be antiviral, antibacterial, antifungal, anti-insect and even poisonous to higher animals including humans (Cowan, 1999;Ng, 2004;Kaur et al, 2006;Singh et al, 2006;Keyaerts et al, 2007;Sitohy et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Conidiogenic effects of mannose-binding lectins isolated from cotyledons of red kidney bean (Phaseolus vulgaris) on Alternaria alternata

Alizadeh¹,

Leung²,

Cole³

2011

Phytochemistry

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Exploration for anti-insect properties of lectin from seeds of soybean (Glycine max) usingBactrocera cucurbitae as a model

Cited by 16 publications

References 25 publications

Are Limits of Concern a useful concept to improve the environmental risk assessment of GM plants?

Are Limits of Concern a useful concept to improve the environmental risk assessment of GM plants?

Monitoring growth, survival and enzyme system of melon fruit fly Bactrocera cucurbitae (Coquillett) under the influence of affinity purified pea lectin

Conidiogenic effects of mannose-binding lectins isolated from cotyledons of red kidney bean (Phaseolus vulgaris) on Alternaria alternata

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