Exploration of ABA Responsive miRNAs Reveals a New Hormone Signaling Crosstalk Pathway Regulating Root Growth of Populus euphratica

Lian, Conglong; Yao, Kun; Duan, Hui; Li, Qing; Liu, Chao; Yin, Weilun; Xia, Xinli

doi:10.3390/ijms19051481

Cited by 25 publications

(8 citation statements)

References 83 publications

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“…A. M. McAdam & Brodribb, 2018;Merilo et al, 2018). ABA signalling is known to affect miRNA production (Lian et al, 2018;Speth, Willing, Rausch, Schneeberger, & Laubinger, 2013;Yan et al, 2017), but to our knowledge, no miRNAs have been demonstrated to affect ABA sensitivity.…”

Section: Introductionmentioning

confidence: 90%

A novel strigolactone‐miR156 module controls stomatal behaviour during drought recovery

Visentin

Pagliarani

Deva

et al. 2020

Plant Cell & Environment

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The Ministry of Education, Youth and Sports, Czech Republic (Grant LO1204 from the National Programme of Sustainability I) supported V.T. and O.N. The PlantStressLab team and E.D. have received funding from the European Union's Horizon 2020 research and innovation programme under the Grant Agreement No. [727929] (TOMRES). None of these funding sources were involved in the design of the study and collection, analysis and interpretation of data, and in writing the manuscript.F.C. agrees to serve as the author responsible for contact and ensures communication. The Ministry of Education, Youth and Sports, Czech Republic (Grant LO1204 from the National Programme of Sustainability I) supported V.T. and O.N. The PlantStressLab team and E.D. have received funding from the European Union's Horizon 2020 research and innovation programme under the Grant Agreement No. [727929] (TOMRES). None of these funding sources were involved in the design of the study and collection, analysis and interpretation of data, and in writing the manuscript. The authors wish to thank Dr. Silva e Ferreira (Sao Paulo, Brazil) for sharing the miR156-oe MicroTom line, Dr. Klee (University of Florida, USA) for the strigolactone-depleted line, and Viviana Cisse and Cecilia Roche for technical support. Word count, main body (Introduction to Acknowledgements): 5976 Number of figures: 6, of which 0 in colour Number of supplementary figures: 1 of which 0 in colour Number of supplementary tables: 1 Main text A novel strigolactone-miR156 module controls stomatal behaviour during drought recovery ABSTRACT miR156 is a conserved microRNA whose role and induction mechanisms under stress are poorly known. Strigolactones are phytohormones needed in shoots for drought acclimation. They promote stomatal closure ABA-dependently and independently; however, downstream effectors for the former have not been identified. Linkage between miR156 and strigolactones under stress has not been reported.We compared ABA accumulation and sensitivity as well as performances of wt and miR156overexpressing (miR156-oe) tomato plants during drought. We also quantified miR156 levels in wt, strigolactone-depleted and strigolactone-treated plants, exposed to drought stress.Under irrigated conditions, miR156 overexpression and strigolactone treatment led to lower stomatal conductance and higher ABA sensitivity. Exogenous strigolactones were sufficient for miR156 accumulation in leaves, while endogenous strigolactones were required for miR156 induction by drought. The "after-effect" of drought, by which stomata do not completely re-open after rewatering, was enhanced by both strigolactones and miR156. The transcript profiles of several miR156 targets were altered in strigolactone-depleted plants.Our results show that strigolactones act as a molecular link between drought and miR156 in tomato, and identify miR156 as a mediator of ABA-dependent effect of strigolactones on the aftereffect of drought on stomata. Thus, we provide insights into both strigolactone and miR156 action on stomata.

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Section: Introductionmentioning

confidence: 90%

A novel strigolactone‐miR156 module controls stomatal behaviour during drought recovery

Visentin

Pagliarani

Deva

et al. 2020

Plant Cell & Environment

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“…Abscisic acid regulates root development also through the action of microRNAs (miRNAs), short single-stranded, small noncoding RNAs that play a major role in plant growth and development, as well as in hormone and stress response (Yan et al, 2017). In Populus euphratica , ABA modulates root growth through miRNA-mediated pathway, together with other hormones, such as GA and brassinosteroids (BRs) (Lian et al, 2018). For instance, peu-miRNA477 was shown to target the repressor of GA1-like (RGL1), a negative regulator of gibberellin response.…”

Section: Abscisic Acidmentioning

confidence: 99%

“…The second example links ABA with BR as BR-insensitive 1 ( BRI1 ) gene was shown to mediate BR signaling response in root growth through interaction with BAK1 (BR-insensitive 1–associated receptor kinase 1 related). Exogenous ABA treatment led to a decrease in the level of peu-miRNA-n68 that targets BAK1, resulting in an induction in BAK1 expression and enhanced root growth (Lian et al, 2018).…”

Section: Abscisic Acidmentioning

confidence: 99%

Apocarotenoids Involved in Plant Development and Stress Response

et al. 2019

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Carotenoids are isoprenoid pigments synthesized by all photosynthetic organisms and many heterotrophic microorganisms. They are equipped with a conjugated double-bond system that builds the basis for their role in harvesting light energy and in protecting the cell from photo-oxidation. In addition, the carotenoids polyene makes them susceptible to oxidative cleavage, yielding carbonyl products called apocarotenoids. This oxidation can be catalyzed by carotenoid cleavage dioxygenases or triggered nonenzymatically by reactive oxygen species. The group of plant apocarotenoids includes important phytohormones, such as abscisic acid and strigolactones, and signaling molecules, such as β-cyclocitral. Abscisic acid is a key regulator of plant's response to abiotic stress and is involved in different developmental processes, such as seed dormancy. Strigolactone is a main regulator of plant architecture and an important signaling molecule in the plant-rhizosphere communication. β-Cyclocitral, a volatile derived from β-carotene oxidation, mediates the response of cells to singlet oxygen stress. Besides these wellknown examples, recent research unraveled novel apocarotenoid growth regulators and suggests the presence of yet unidentified ones. In this review, we describe the biosynthesis and biological functions of established regulatory apocarotenoids and touch on the recently identified anchorene and zaxinone, with emphasis on their role in plant growth, development, and stress response.

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“…MiRNAs negatively regulate their target gene expression at transcriptional and post-transcriptional levels by regulating both messenger RNA (mRNA) degradation and translational inhibition based on miRNA/target sequence complementarity [6]. High-throughput degradome sequencing has been successfully established and adapted to validate miRNA splicing targets in a variety of plant species, such as hyperaccumulator Sedum alfredii [21], Populus [22], rice [23,24], soybean [25], canola [5], and maize [26]. Recently, an integrated web-based tool, DPMIND (Degradome-based Plant MiRNA-Target Interaction and Network Database), was developed to scan sRNA targets in multiple plant species [27].…”

Section: Introductionmentioning

confidence: 99%

Bioinformatic Exploration of the Targets of Xylem Sap miRNAs in Maize under Cadmium Stress

Wang

Cheng

Chen

et al. 2019

IJMS

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Cadmium (Cd) has the potential to be chronically toxic to humans through contaminated crop products. MicroRNAs (miRNAs) can move systemically in plants. To investigate the roles of long-distance moving xylem miRNAs in regulating maize response to Cd stress, three xylem sap small RNA (sRNA) libraries were constructed for high-throughput sequencing to identify potential mobile miRNAs in Cd-stressed maize seedlings and their putative targets in maize transcriptomes. In total, about 199 miRNAs (20–22 nucleotides) were identified in xylem sap from maize seedlings, including 97 newly discovered miRNAs and 102 known miRNAs. Among them, 10 miRNAs showed differential expression in xylem sap after 1 h of Cd treatment. Two miRNAs target prediction tools, psRNAtarget (reporting the inhibition pattern of cleavage) and DPMIND (discovering Plant MiRNA-Target Interaction with degradome evidence), were used in combination to identify, via bioinformatics, the targets of 199 significantly expressed miRNAs in maize xylem sap. The integrative results of these two bioinformatic tools suggested that 27 xylem sap miRNAs inhibit 34 genes through cleavage with degradome evidence. Moreover, nearly 300 other genes were also the potential miRNAs cleavable targets without available degradome data support, and the majority of them were enriched in abiotic stress response, cell signaling, transcription regulation, as well as metal handling. These approaches and results not only enhanced our understanding of the Cd-responsive long-distance transported miRNAs from the view of xylem sap, but also provided novel insights for predicting the molecular genetic mechanisms mediated by miRNAs.

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Exploration of ABA Responsive miRNAs Reveals a New Hormone Signaling Crosstalk Pathway Regulating Root Growth of Populus euphratica

Cited by 25 publications

References 83 publications

A novel strigolactone‐miR156 module controls stomatal behaviour during drought recovery

A novel strigolactone‐miR156 module controls stomatal behaviour during drought recovery

Apocarotenoids Involved in Plant Development and Stress Response

Bioinformatic Exploration of the Targets of Xylem Sap miRNAs in Maize under Cadmium Stress

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