Exploration of Antibacterial and Antioxidative Property of Two Natural Honey Samples from Malda District, India

Abstract

Aims: The current study determines the antibacterial activity of 'Bakul' (Mimusops elengi) leaf, seed and bark extracts against gram-negative clinical bacterial isolates as well as the standard bacterial strains. Methods: The disc diffusion method was followed to determine the antibacterial activity of M. elengi leaf, seed and bark extracts against the clinical isolates of Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris and Pseudomonas aeruginosa. The antibiotic susceptibility of the bacterial isolates was determined by disc diffusion. Results: The concentration dependent activity of the extracts against the bacteria was recorded with zone diameter of inhibition 7-21 mm. The extracts in combination with antibiotics (cefpodoxime, gentamycin and ciprofloxacin) had growth inhibitory indices (GIIs), 0.5-0.6, 0.5-0.89 and 0.73-0.82 against E. coli, K. pneumoniae and Pr. vulgaris clinical isolates, respectively, while for the standard strains (E. coli, K. pneumoniae and Ps. aeruginosa) the GIIs ranged 0.56-0.86. Most of the extracts were tested positive for the presence of flavonoids, glycosides, steroids, terpenoids, quinone and phenol. Conclusion: The M. elengi leaf, seed and bark extracts, in combination with antibiotics, had synergistic interactions against all the standard bacterial strains and Pr. vulgaris clinical isolate, while both synergistic and additive interactions were recorded against E. coli and K. pneumoniae clinical isolates.

Antibacterial Activity of Honey on Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes Isolated from Wounds

“…The study which was carried out to assess the antibacterial activity of honey from Ishielu Local Government Area of Ebonyi State on some pathogenic microorganisms (Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes) isolated from wounds, confirmed the antibacterial efficacy of honey against the isolated bacterial species. The study also showed Staphylococcus aureus to be the most predominant bacterial species isolated from infected wounds, which is consistent with the study of Aynalem et al [20], Surajit et al [21] and Okeke et al [22]. However, this study varied with the report of Farrag et al [23] who reported Pseudomonas species as the most common isolates.…”

Applicability of Honey on Silkworms (Bombyx mori) and Quality Improvement of Its Biomaterials

“…With its extraordinary features, such as the antibacterial, antioxidative and antiinflammatory effects [62,84], honey is currently used to enhance the silk fibroin-based biomaterials that are used in medical practice. There have been several silk fibroin honeyenhanced biomaterials developed, such as Manuka honey-enhanced silk fibroin matrices, silk fibroin cryogels that contain Manuka honey and honey-blended silk fibroin membranes [123,125].…”

“…Besides the antibacterial activity, honey possesses another great characteristic when it comes to its biomedical applications, namely, it exhibits antioxidative effects [84]. The antioxidative effect has an important role by counteracting the action of oxidant compounds [85].…”

Antibacterial potential of hive bees honey from Himachal Pradesh, India

“…This discrepancy in the antibacterial potency against selected pathogens could be ascribed to the chemistry of honey (its acidic pH 3.2-4.5, high osmolarity, hydrogen peroxide, non-peroxide factors), its phytochemical nature (content of phenols, tetracycline derivatives, amylase, streptomycin, sulfathiazole, fatty acids, ascorbic acid, flavonoides, trepens, benzyl alcohol and benzoic acids, etc. ), and geographic and seasonal (spatial and temporal) variation in honey collection (Mohapatra et al 2011;Tada et al 2012;Radhakrishnan et al 2014;Girotra et al 2016;Roy et al 2016). Further, there may exist a correlation between the microbial diversity and antibacterial potential of the hive bee's honey.…”