Exploration of glutathione reductase for abiotic stress response in bread wheat (Triticum aestivum L.)

Madhu,; Kaur, Amandeep; Tyagi, Shivi; Shumayla, .; Singh, Kashmir; Upadhyay, Santosh Kumar

doi:10.1007/s00299-021-02717-1

Cited by 55 publications

(30 citation statements)

References 99 publications

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“…The results were analyzed in the form of fold expression change by the delta-delta CT method (2 −ΔΔCT ) [ 75 ]. The expression of the ADP-ribosylation factor ( TaARF1 ) was used as an internal control [ 76 , 77 ]. All the experiments were performed in three biological replicates and expressed as mean ± SD.…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization, Evolutionary Analysis, and Expression Profiling of BOR Genes in Important Cereals

Sharma

Rajput

et al. 2022

Plants

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Boron (B) is an essential micronutrient of plants. Plants grapple with a narrow range of B between its toxicity and deficiency. B homeostasis mechanism is required to rescue plants from such a quagmire. B transporters are specialized proteins involved in the homeostasis of B. In the present study, a total of 29 BOR genes were identified in five major cereals, including three BORs in each Brachypodium distachyon and Sorghum bicolor, four in Oryza sativa, six in Zea mays, and 13 in Triticum aestivum. Multiple sequence alignments, domain structure analyses, and phylogenetic analysis indicated the conserved nature of the BOR protein family. Duplication events and Ka/Ks analysis of TaBORs showed the role of segmental duplication events and purifying selection in the expansion of the BOR family in T. aestivum. Furthermore, in silico expression and co-expression analyses under biotic and abiotic stress conditions depicted their involvement in combating such conditions. Moreover, qRT-PCR of TaBORs in B treatment suggested the roles of BOR genes in B stress management. The present study hints at the conserved nature of BOR proteins and their different aspects. The study will lay down a way for several crop improvement programs.

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Section: Methodsmentioning

confidence: 99%

Molecular Characterization, Evolutionary Analysis, and Expression Profiling of BOR Genes in Important Cereals

Sharma

Rajput

et al. 2022

Plants

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“…A total of seven GR proteins were identified in A. sativa , which is the same number as that in bread wheat and more than those in other species. It suggested that the GR family members are closely related to their genome duplication events and genome size [ 24 ]. GR proteins were initially classified into cytosolic and chloroplast types based on the presence of a typical chloroplast signal peptide at the N-terminus.…”

Section: Discussionmentioning

confidence: 99%

“…The GR protein encoded by a small gene family has been described in many crops and model plants, such as Arabidopsis thaliana , Brassica juncea , Brassica rapa , Oryza sativa , Populus trichocarpa , Aegilops tauschii , and Triticum aestivum [ 15 , 17 , 23 , 24 ]. The genome-wide identification of GRs , and characterizing its structure and function, would be useful for future functional and mechanism studies on GR proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Typically, two or three GRs are found in the above reported species. Recently, seven GR genes were identified in the allohexaploid genome (AABBDD) of bread wheat [ 24 ]. Oats ( Avena sativa ) are also an allohexaploid species (AACCDD, 2 n = 6 x = 42) and rank sixth in world cereal production due to high nutritional and medicinal value and broad distributions [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition

Sun

Jia

et al. 2022

IJMS

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Abiotic stress disturbs plant cellular redox homeostasis, inhibiting seed germination and plant growth. This is a crucial limitation to crop yield. Glutathione reductase (GR) is an important component of the ascorbate-glutathione (AsA-GSH) cycle which is involved in multiple plant metabolic processes. In the present study, GRs in A. sativa (AsGRs) were selected to explore their molecular characterization, phylogenetic relationship, and RNA expression changes during seed imbibition under abiotic stress. Seven AsGR genes were identified and mapped on six chromosomes of A, C, and D subgenomes. Phylogenetic analysis and subcellular localization of AsGR proteins divided them into two sub-families, AsGR1 and AsGR2, which were predicted to be mainly located in cytoplasm, mitochondrion, and chloroplast. Cis-elements relevant to stress and hormone responses are distributed in promoter regions of AsGRs. Tissue-specific expression profiling showed that AsGR1 genes were highly expressed in roots, leaves, and seeds, while AsGR2 genes were highly expressed in leaves and seeds. Both AsGR1 and AsGR2 genes showed a decreasing-increasing expression trend during seed germination under non-stress conditions. In addition, their responses to drought, salt, cold, copper, H2O2, and ageing treatments were quite different during seed imbibition. Among the seven AsGR genes, AsGR1-A, AsGR1-C, AsGR2-A, and AsGR2-D responded more significantly, especially under drought, ageing, and H2O2 stress. This study has laid the ground for the functional characterization of GR and the improvement of oat stress tolerance and seed vigor.

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“…The exposure of transgenic seedlings of P. glaucoma to varying stress regiments exhibited an escalation in the antioxidant enzyme activity and stress tolerance (Raja et al 2021). Madhu et al (2021) identified seven glutathione reductase (GR) genes in wheat (Triticum aestivum) for comparative structural characterization, phylogenetic analysis and expression profiling with the GR genes of other cereal plants. The differential expression of TaGR genes and enhanced GR enzyme activity have important functions under drought, heat, salt and arsenic stress.…”

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confidence: 99%

Hot and dry: how plants can thrive in future climates

2022

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Exploration of glutathione reductase for abiotic stress response in bread wheat (Triticum aestivum L.)

Cited by 55 publications

References 99 publications

Molecular Characterization, Evolutionary Analysis, and Expression Profiling of BOR Genes in Important Cereals

Molecular Characterization, Evolutionary Analysis, and Expression Profiling of BOR Genes in Important Cereals

Genome-Wide Analysis and Expression Profiling of Glutathione Reductase Gene Family in Oat (Avena sativa) Indicate Their Responses to Abiotic Stress during Seed Imbibition

Hot and dry: how plants can thrive in future climates

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