Exploration of Immunogenic Cell Death-Associated Genes for Predicting Prognosis and Immunological Characteristics in Cervical Squamous Cell Carcinoma

Abstract

Background. The tumor microenvironment (TME) has gradually entered the vision of researchers and is becoming a vital part of the occurrence of cervical squamous cell carcinoma (CSCC). However, understanding the specific composition of TME still confront enormous challenges, particularly immune and stromal components. Methods. In this study, we performed an unsupervised cluster analysis to determine the immunogenic cell death-associated subtype of CSCC patients. The differences in immune status, genomic alteration, and clinical outcomes between each subtype were compared. Subsequently, we screened vital prognostic factors. The HPA database was employed to verify the protein localization and the expression level between cancer and adjacent tissues. Results. CSCC patients were divided into three subtypes according to the expression of immunogenic cell death-associated genes. Cluster C has the highest survival rate because of the lower activation of tumor-related pathways. The immune score and stromal score of patients with Cluster B were the highest, so it may be considered that stromal tissue inhibits the anti-tumor effect of immunocytes. In addition, we constructed a risk score based on immunogenic cell death-associated genes to screen for vital markers. We systematically revealed the genomic alteration of vital markers. Conclusions. We have established a novel immunogenic cell death-associated risk scoring system in CSCC, and the expression of immunogenic cell death-associated genes may be a valuable biomarker for immunotherapy strategies. Our work may contribute to the development of new immunomodulators and develop new precision immunotherapies for CSCC.