Exploration of indigenous soil bacteria producing-exopolysaccharides for stabilizing of aggregates land potential as biofertilizer

Arfarita, Novi; Hidayati, Nur; Rosyidah, Anis; Machfudz, Masyhuri; Higuchi, Takaya

doi:10.15243/jdmlm.2016.041.697

Cited by 15 publications

(8 citation statements)

References 7 publications

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“…While the research stage for VP3 biofertilizers, begins with exploration of soil bacteria from the Malang area and obtained three indigenous bacteria, namely N-free bacteria Bacillus licheniformis, phosphate solubilizing bacteria Pantoea ananatis, and exopolysaccharide-producing bacteria Pseudomonas plecoglossicida [4][5][6]. VP3 Biofertilizer is a liquid formulation made from vermiwash and molasses as carriers, then added PEG and 3 functional bacterial isolates [7].…”

Section: Introductionmentioning

confidence: 99%

Field application of VP3 biofertilizer on soybeans (Glycine max L.) and yield comparison between four biofertilizers sold in the market

Arfarita

2022

IOP Conf. Ser.: Earth Environ. Sci.

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The VP3 biofertilizer formulation begins with exploration of indigenous soil bacteria isolated from several soil samples in Malang-Indonesia. The VP3 Biofertilizer is a liquid formulation made from vermiwash and molasses as carriers, with 3 functional bacterial isolates. It has been proven to be able to increase the yield of mung beans, long yard beans, and green beans in the green house, however it has not been applied to soybean (Glycine max L.) in the field compared to other biofertilizers on the market. This study used soybean seeds of the Anjasmoro variety and consisted of 6 treatments (different types of biofertilizer) with a Randomized Block Design (RBD) namely VP3 biofertilizer, EM4 biofertilizer, Sumber Subur biofertilizer, Semanggi biofertilizer, and Magicgro G6 biofertilizer. This research was carried out in idle land. The application of VP3 biofertilizer when compared to other biofertilizers on the market generally gave better results on the parameters of growth and yield of soybeans in the field. This is indicated by the significant yield on leaf area per plant and yield of total seed weight per plot of soybean plant.

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Section: Introductionmentioning

confidence: 99%

Field application of VP3 biofertilizer on soybeans (Glycine max L.) and yield comparison between four biofertilizers sold in the market

Arfarita

2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Understanding of the rhizosphere nice and microbial community interactions will assist development of inoculants across farming systems with potentially greater consistency in performance and survival, especially the use of indigenous microorganisms (Torsvik and Øvreås, 2002). The use of indigenous microorganisms is more sustainable than the introduction of microorganisms when applied as a biological fertilizer or for bioremediation (Nuraini et al, 2015;Arfarita et al, 2016;Arfarita et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna radiata for agricultural land treatment

Arfarita

Muhibuddin

Imai

2019

J.Degrade.Min.Land Manage.

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Nitrogen is the most abundant element in the atmosphere, however, most often deficient in agricultural lands. This research was an exploratory to get indigenous non-symbiotic nitrogen-fixing bacteria. Soil samples were collected from rhizosphere of green beans. This study was aimed to determine the bacterial population of the three regions; screening, isolation and selection of free nitrogen-fixing bacteria. Antagonism and pathogenicity tests were performed to observe its potential for a biofertilizer product. The highest number of free nitrogen-fixing bacteria was found from forest soil sample of 2.5x 10 11 CFU/ml. Screening and isolation process has obtained 10 free nitrogen-fixing isolates. Then was selected into 4 isolates namely SNF4, SNF5, SNF7 and SNF8 according to the ammonia production test qualitatively. When an antagonism activity performed, there was no inhibition zone each other. The pathogenicity test did not show the pathogenic symptom. This study also showed that bacterial isolates obtained significantly affected the germination growth of green beans compared to controls. Possibility, bacteria of this type produced growth hormone for a plant. Strain SNF8 has shown the highest ammonium production then was selected for 16S rRNA identification. Similarity test of genome sequence of strain SNF8 had 99% similarity with Bacillus cereus.

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“…In this study, a liquid formulation of Biological fertilizer made from seaweed waste was added by three indigenous bacterial inoculants obtained from previous studies (Arfarita et al, 2016;2017;2019), namely: Pseudomonas plecoglossicida which acts as exopolysaccharideproducing bacteria to enhance soil aggregation, Bacillus licheniformis which acts as a free N fixing bacteria and Pantoea ananatis which acts as a phosphate soluble bacteria. The use of all three bacterial indigenous isolates is important to improve nutrient availability, so that when biofertilizers are being applied in specific conditions of agricultural area, it can be repeated to the other regions, as long as it has similar climatic and soil conditions when biological condition of this fertilizers can be maintained in optimum condition.…”

Section: Introductionmentioning

confidence: 99%

Effects of seaweed waste on the viability of three bacterial isolates in biological fertilizer liquid formulations to enhance soil aggregation and fertility

Arfarita¹,

Higuchi²,

Prayogo³

2019

J.Degrade.Min.Land Manage.

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Biofertilizer production in Indonesia should fulfil the minimum requirement for being produced and released to the market. Problems occurred when those products are being absent on informing those expiration dates and the viability of microbial activity which then closely related to the quality of the product. Seaweed composted material are potential resources for producing Biofertilizer, but lacking on the optimization on their process as this material contain a various important component for soil and environment. The production of Biofertilizer from seaweed waste required an optimum condition, i.e.: pH and typical microbe which could germinate under specific formulation and temperature. This study aimed to determine the optimum pH in liquid fertilizer formulations made from seaweed waste in the form of composted material, to test the viability of three bacterial isolates and those pathogenicity properties, to examine the effect of metabolites release from bacterial isolates to green bean seed germination. The experimental design used was a completely randomized design with four treatments, which were as follow: P0 as a control (Peptone), RP1 (seaweed waste), RP2 (seaweed waste and glycerol), and RP3 (seaweed waste and PEG). The three bacterial isolates used were: (1) Bacillus licheniformis, (2) Psudomonas plecoglossicida and (3) Pantoea ananatis. This liquid fertilizer biological formulation was stored for 8 weeks at pH 5.5 and temperature 25 o C. The results showed that the treatment of RP1 (seaweed waste) had high bacterial viability and could stimulate growth for green bean sprouts. The carrier material for seaweed waste with the addition of glycerol and PEG showed no effect of the disease and symptoms of a pathogenic bacterial consortium on germination of green beans.

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Exploration of indigenous soil bacteria producing-exopolysaccharides for stabilizing of aggregates land potential as biofertilizer

Abstract: :Steady soil aggregationis important for agricultural land which is formed by the micro-

Cited by 15 publications

References 7 publications

Field application of VP3 biofertilizer on soybeans (Glycine max L.) and yield comparison between four biofertilizers sold in the market

Field application of VP3 biofertilizer on soybeans (Glycine max L.) and yield comparison between four biofertilizers sold in the market

Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna radiata for agricultural land treatment

Effects of seaweed waste on the viability of three bacterial isolates in biological fertilizer liquid formulations to enhance soil aggregation and fertility

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